Analysis of Genetic Diversity and Structure of Guanzhong Horse Using Microsatellite Markers.
Abstract: To determine the genetic diversity and validate the pedigree record of Chinese Guanzhong horse, 67 individuals were genotyped with eight microsatellite markers. In our study, the mean observed and expected heterozygosities were 0.51 and 0.66, respectively. The mean observed number of alleles for the Guanzhong horse was 3.88. Nonetheless, the total value of F multiloci clearly indicates that about 0.5% of overall genetic variation is due to line founder differences, while differences among individuals are responsible for the remaining 99.5%. In addition, the polymorphic information content (PIC) result showed that five loci (HTG7, HMS7, HMS2, AHT4, and HMS6) were highly polymorphic (PIC > 0.5) and three loci (HMS3, HTG6, and COR071) were moderate polymorphic (PIC > 0.25). Genetic distances and cluster analysis showed that the genetic relationship among 67 Guanzhong horse was generally consistent with pedigree recorded. Our results not only evaluated the genetic diversity of Chinese Guanzhong horse, but also suggested that the eight microsatellite markers might be used as subservient markers for parentage verification and individual identification in the Guanzhong horse.
Publication Date: 2018-02-20 PubMed ID: 29463179DOI: 10.1080/10495398.2017.1416392Google Scholar: Lookup
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Summary
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The research conducted a genetic analysis of the Guanzhong horse breed in China, finding that the breed has a diverse genetic structure, which is consistent with its known pedigree record. The research undertook genotyping of the breed and concluded that microsatellite markers could be used in the future for verifying parentage and identifying individual horses within this breed.
Analysis of Genetic Diversity and Structure
- This study involved the genotyping of 67 Chinese Guanzhong horses. Genotyping is a process used in genetics research to determine differences in the genetic makeup (genotype) of an individual by examining their DNA sequence. This was accomplished using eight specific microsatellite markers.
- The research found that there was a mean observed heterozygosity of 0.51 and an expected heterozygosity of 0.66 across all samples. Heterozygosity refers to the presence of different alleles for a particular genetic trait. This suggests that there is a significant amount of genetic variation within the breed, a potential indication of its genetic health.
- The mean observed number of alleles was 3.88, providing further evidence of genetic diversity.
Genetic Variation with Individual Differences
- The F multiloci estimation discovered that only 0.5% of the total genetic variation was attributable to differences in lineage founders. This suggests that the majority of the genetic diversity (99.5%) lies in differences amongst individuals within the breed rather than between different lineages or families.
Polymorphic Information Content and Identification Potential
- The genes studied in the research were categorized based on their polymorphic information content (PIC), a measure of the diversity of a specific gene locus in the population.
- Among the eight studied genes, five (HTG7, HMS7, HMS2, AHT4, and HMS6) were found to be highly polymorphic, meaning they had a PIC greater than 0.5. This indicates high genetic diversity at these loci.
- Three loci (HMS3, HTG6, and COR071) showed moderate polymorphism with a PIC value greater than 0.25.
- The research suggests that these polymorphic genes may be useful for verifying parentage and identifying individual horses within the Guanzhong breed, making them potentially valuable tools for breed management and conservation.
Consistency with Pedigree Records
- A comparison of the genetic relationships inferred from the genetic data with the known pedigree records of the sampled horses showed broad consistency. This validation provides support for the use of genetic analysis in breed management and conservation.
Cite This Article
APA
Zeng L, Chen N, Yao Y, Dang R, Chen H, Lei C.
(2018).
Analysis of Genetic Diversity and Structure of Guanzhong Horse Using Microsatellite Markers.
Anim Biotechnol, 30(1), 95-98.
https://doi.org/10.1080/10495398.2017.1416392 Publication
Researcher Affiliations
- a College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi , China.
- a College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi , China.
- a College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi , China.
- a College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi , China.
- a College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi , China.
- a College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi , China.
MeSH Terms
- Alleles
- Animals
- Breeding
- Cluster Analysis
- Conservation of Natural Resources
- Female
- Genetic Markers / genetics
- Genetic Variation
- Genotype
- Genotyping Techniques / veterinary
- Horses / genetics
- Male
- Microsatellite Repeats / genetics
- Pedigree
Citations
This article has been cited 6 times.- Wang T, Liu Z, Shi X, Zhang Z, Li Y, Huang B, Ren W, Wang X, Wang C, Chai W. An investigation of genetic diversity in three Dezhou donkey original breeding farms. Sci Rep 2023 Jul 11;13(1):11203.
- Meng S, Zhang Y, Lv S, Zhang Z, Liu X, Jiang L. Comparison of muscle metabolomics between two Chinese horse breeds. Front Vet Sci 2023;10:1162953.
- Yordanov G, Mehandjyiski I, Palova N, Atsenova N, Neov B, Radoslavov G, Hristov P. Genetic Diversity and Structure of the Main Danubian Horse Paternal Genealogical Lineages Based on Microsatellite Genotyping. Vet Sci 2022 Jul 1;9(7).
- Hsiao C, Lin HH, Kang SR, Hung CY, Sun PY, Yu CC, Toh KL, Yu PJ, Ju YT. Development of 16 novel EST-SSR markers for species identification and cross-genus amplification in sambar, sika, and red deer. PLoS One 2022;17(4):e0265311.
- Funk SM, Guedaoura S, Juras R, Raziq A, Landolsi F, Luís C, Martínez AM, Musa Mayaki A, Mujica F, Oom MDM, Ouragh L, Stranger YM, Vega-Pla JL, Cothran EG. Major inconsistencies of inferred population genetic structure estimated in a large set of domestic horse breeds using microsatellites. Ecol Evol 2020 May;10(10):4261-4279.
- Hou L, Sulayman A, Zeng Y, Zhou L, Aimaier A, Kader A, Shi L. Analysis of Genetic Diversity and Race Genetic Structure of Major Horse Breeds in Xinjiang, China. Animals (Basel) 2025 Sep 14;15(18).
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