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Home / Equine Research Bank / Vet Parasitol / Analysis of multiyear studies in horses in Kentucky to ascer...
Veterinary parasitology2010; 174(1-2); 77-84; doi: 10.1016/j.vetpar.2010.08.007

Analysis of multiyear studies in horses in Kentucky to ascertain whether counts of eggs and larvae per gram of feces are reliable indicators of numbers of strongyles and ascarids present.

Abstract: Increasing levels of anthelmintic resistance in equine nematodes have led to recommendations of more sustainable anthelmintic treatment protocols with emphasis on parasite surveillance and diagnosis, rather than prophylactic calendar-based treatments. This requires knowledge of the diagnostic test performance of techniques for counts of eggs per gram of feces (EPG) as well as methods for culturing, counting and identifying third stage (L(3)) strongyle larvae per gram of feces (LPG). For horses, such information does not exist in the published literature. The aim of this study was to examine the relationship between worm count and fecal egg count (FEC) data for strongyle and Parascaris equorum infections as well as larval culture counts for diagnosing Strongylus spp. infections. Necropsy data from 693 horses used for critical or controlled tests, including information on total worm counts, fecal egg counts (FEC) and larval culture results collected at the University of Kentucky over a period of 50 years were analyzed. Sensitivity, specificity, positive and negative predictive values, and receiver operator characteristic (ROC) curves were generated for the larval cultures and ascarid egg counts. For the strongyle egg counts, potential FEC cutoff values for treatment were evaluated statistically by comparing the total strongyle worm counts below and above chosen cutoff values. All tests had high positive predictive values (>0.95), but moderate negative predictive values (<0.70). The negative predictive values of the larval counts were negatively affected by increasing egg count levels. Strongyle FEC cutoff values up to the level of 500 EPG yielded significantly higher strongyle worm counts in the treatment group, whereas no differences were found at higher cutoffs. This supports usage of cutoffs for treatment in the 0-500 EPG range. Altogether, the present study yields unique and useful information of widely used methods for parasite surveillance and diagnosis in equine establishments.
Copyright © 2010 Elsevier B.V. All rights reserved.
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Publication Date: 2010-08-17 PubMed ID: 20850927DOI: 10.1016/j.vetpar.2010.08.007Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study examines the relationship between the number of worms in horses and the count of their eggs in faeces, with the aim to improve parasitic surveillance and diagnosis in equine care. The findings suggest that taking counts of eggs in faeces can indeed serve as a reliable method in diagnosing worm infections, provided the considered egg count falls within a certain range.

Background

  • This research is necessitated by increasing levels of resistance in equine nematodes to anthelmintic, the drugs used for their treatment. As a result, the treatment strategy has pivoted from prophylactic calendar-based treatments to more sustainable surveillance and diagnosis-centric treatments.
  • The study’s focus is on the efficiency of diagnostic tests for counts of eggs per gram of faeces (EPG) along with methods for counting and identifying strongyle larvae in faeces. The lack of such information on horses led to this research.
  • The study aims to understand the relationship between total worm count and fecal egg count for both strongyle and Parascaris equorum infections, and counts of cultured larvae for diagnosing Strongylus spp. infections.

Methodology

  • The study analysed data from 693 necropsies conducted over 50 years on horses at the University of Kentucky. The data contained information regarding total worm counts, fecal egg counts and results of larval culture.
  • Several measures of test effectiveness like sensitivity, specificity, positive and negative predictive values, and receiver operator characteristic (ROC) curves were generated for egg counts of ascarids and larval cultures.
  • Potential thresholds for treatment based on strongyle egg counts were also statistically evaluated by comparing total strongyle worm counts below and above the established values.

Findings

  • All tests had high positive predictive values (greater than 0.95); however, the negative predictive values were moderate (less than 0.70).
  • The study’s negative predictive values were affected negatively with increasing egg count levels.
  • Strongyle fecal egg count thresholds up to the level of 500 EPG resulted in significantly higher strongyle worm counts in horses needing treatment. No differences were found at higher thresholds.
  • This implies that for strongyle egg counts in the 0-500 EPG range, it is beneficial to use these counts for determining treatment.

Conclusion

  • The research provides unique information about the widely used methods for parasitic surveillance and diagnosis in horse establishments. It concludes that counts of eggs per gram of faeces can serve as a reliable diagnostic method for worm infections in horses, provided the egg count is in the considered range.

Cite This Article

APA
Nielsen MK, Baptiste KE, Tolliver SC, Collins SS, Lyons ET. (2010). Analysis of multiyear studies in horses in Kentucky to ascertain whether counts of eggs and larvae per gram of feces are reliable indicators of numbers of strongyles and ascarids present. Vet Parasitol, 174(1-2), 77-84. https://doi.org/10.1016/j.vetpar.2010.08.007

Publication

Veterinary parasitology
ISSN: 1873-2550
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 174
Issue: 1-2
Pages: 77-84

Researcher Affiliations

Nielsen, M K
  • Department of Large Animal Sciences, Faculty of Life Sciences, University of Copenhagen, 5 Højbakkegård Alle, DK-2630 Taastrup, Denmark. mkn@life.ku.dk
Baptiste, K E
    Tolliver, S C
      Collins, S S
        Lyons, E T

          MeSH Terms

          • Animals
          • Ascaridida Infections / diagnosis
          • Ascaridida Infections / veterinary
          • Ascaridoidea
          • Feces / parasitology
          • Horse Diseases / diagnosis
          • Horse Diseases / parasitology
          • Horses
          • Kentucky
          • Larva
          • Parasite Egg Count / statistics & numerical data
          • Parasite Egg Count / veterinary
          • Strongyle Infections, Equine / diagnosis
          • Strongyloidea / isolation & purification

          Citations

          This article has been cited 34 times.
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