Analytical Validation of MINI-PET as Point-of-Care for Erythrocyte Sedimentation Rate Measure in Horses.
Abstract: The erythrocyte sedimentation rate (ESR) is a widely used diagnostic assay in human medicine but nowadays poorly applied in veterinary medicine. This test measures the speed (millimeters per hour) at which red blood cells settle in a whole anticoagulated blood tube. In human medicine, high ESR values are associated with various disorders, including infections, rheumatoid arthritis, oncologic diseases, and other inflammatory conditions. The ESR can also be influenced by some factors such as age and gender. In veterinary medicine, the ESR with the Westergren manual method was almost forgotten over the years due to blood consumption and long turn-around time. The instrument MINI-PET, using a modified Westergren method, does not require blood consumption or release waste product and recently has been applied in canine medicine. The aims of the study in the horse were as follows: to establish the appropriate time to read the ESR with the Westergren reference method; to compare the MINI-PET ESR results with the reference technique; to assess the ESR reference intervals with MINI-PET; and to establish the ESR stability from collection at different time points by MINI-PET. Using 150 horses, we established 60 minutes as the appropriate time for ESR reading with the Westergren method. Moreover, ESR results obtained in 8 minutes with MINI-PET showed a good correlation with the Westergren ESR. Reference intervals (RIs) with MINI-PET were established in mm/h for the healthy horses (geldings 18.6-100.1; stallions, 13.8-55.7; and mares 1-73.7) according to the American Society of Veterinary Clinical Pathology guidelines. In addition, the ESR stability from the blood collection time was evaluated in the MINI-PET on 15 horses: at room temperature, ESR is stable up to 8 hours and at 4°C up to 24 hours. In conclusion, MINI-PET represents a rapid and reliable tool for measuring ESR in horses, offering a valid option to replace the traditional manual technique.
Copyright © 2023 Carolina Pieroni et al.
Publication Date: 2023-11-15 PubMed ID: 38023429PubMed Central: PMC10665103DOI: 10.1155/2023/9965095Google Scholar: Lookup
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Summary
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This research explores the validation of the MINI-PET tool for measuring the erythrocyte sedimentation rate (ESR) in horses, offering a faster and more reliable technique.
Understanding Erythrocyte Sedimentation Rate (ESR)
- ESR refers to the speed at which red blood cells settle in a tube of anticoagulated whole blood, measured in millimeters per hour.
- Used commonly in human medicine, high ESR values are associated with various disorders such as infections, rheumatoid arthritis, cancer, and other inflammatory conditions.
- Factors like age and gender can influence the ESR.
- However, in veterinary medicine, ESR measurement using the traditional Westergren method has not been widely adopted due to issues such as blood consumption and extended time required for results.
The MINI-PET Tool and Its Advantages
- This study evaluates the efficacy of the MINI-PET instrument, which utilizes a modified Westergren method to measure ESR in horses.
- Unlike the traditional technique, MINI-PET does not consume blood or produce waste.
- Previously, this instrument has been successfully used in canine medicine.
Major Findings from the Study
- The study was conducted using 150 horses to validate the use of MINI-PET in ESR measurement.
- The researchers identified that 60 minutes was the appropriate time for ESR reading with the Westergren method.
- It was found that ESR results achieved in 8 minutes with MINI-PET correlated well with those obtained from the traditional Westergren ESR measurement.
- Reference intervals were also established using MINI-PET for healthy horses. For geldings, stallions, and mares, the intervals were 18.6-100.1 mm/h, 13.8-55.7 mm/h, and 1-73.7 mm/h, respectively.
- The study also looked into the ESR stability from the time of blood collection. In room temperature, ESR remained stable up to 8 hours, and at 4°C, it was stable up to 24 hours.
Final Conclusion
- In conclusion, the study validates the MINI-PET as a quick and reliable tool for ESR measurement in horses.
- This instrument provides an efficient alternative to the traditional manual technique, potentially encouraging the wider adoption of ESR testing in veterinary medicine.
Cite This Article
APA
Pieroni C, Grassi A, Pantoli M, Berretti M, Messina S, Giovannini C, Lubas G, Diamanti D.
(2023).
Analytical Validation of MINI-PET as Point-of-Care for Erythrocyte Sedimentation Rate Measure in Horses.
Vet Med Int, 2023, 9965095.
https://doi.org/10.1155/2023/9965095 Publication
Researcher Affiliations
- DIESSE- Diagnostica Senese S.p.A. Società Benefit, Strada dei Laghi, 35-39, Monteriggioni, Siena, Italy.
- I-Vet Diagnostica Veterinaria, Via Ettore Majorana, 10, Flero, Brescia, Italy.
- Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Sede Territoriale di Pavia, Str. Privata Campeggi, 59, Pavia, Italy.
- I-Vet Diagnostica Veterinaria, Via Ettore Majorana, 10, Flero, Brescia, Italy.
- DIESSE- Diagnostica Senese S.p.A. Società Benefit, Strada dei Laghi, 35-39, Monteriggioni, Siena, Italy.
- Azienda Ospedaliera Universitaria Senese, V.le Mario Bracci, 11, Siena, Italy.
- Il Ceppo Equine Hospital, Strada Monteresi 3, Monteriggioni, Siena, Italy.
- Clinica Veterinaria Colombo, VetPartners Italia, V.le Colombo 153, Lido di Camaiore, Lucca, Italy.
- DIESSE- Diagnostica Senese S.p.A. Società Benefit, Strada dei Laghi, 35-39, Monteriggioni, Siena, Italy.
Conflict of Interest Statement
The authors declare the following financial interests/personal relationships which may be considered as a potential conflict of interest: DIESSE Diagnostica Senese S.p.A. Società Benefit provided the MINI-PET ESR instrument. Dr. Carolina Pieroni, Dr. Daniela Diamanti, and Dr. Mirko Berretti are DIESSE Diagnostica Senese S.p.A. Società Benefit members. None of the other authors has any other financial or personal relationship that could inappropriately influence or bias the content of the paper.
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