Aquaporin water channels AQP1 and AQP3, are expressed in equine articular chondrocytes.
Abstract: Chondrocytes exist in an unusual and highly variable ionic and osmotic environment in the extracellular matrix of articular cartilage. Alterations to the ionic and osmotic environment of chondrocytes influence the volume and ionic content of the cells, which, in turn, modifies the rate at which extracellular matrix macromolecules are synthesized and degraded. Thus, regulation of the water and solute content of chondrocytes will profoundly affect their anabolic and catabolic functions. The water content of cells is effectively influenced by the abundance of aquaporin (AQP) water channels. Recent studies have shown that several AQP water channel isoforms are expressed in chondrocytes from Meckel's cartilage, developing teeth and other orofacial tissues. The aim of the present investigation was to determine if chondrocytes from equine articular cartilage express AQP water channels. Polyclonal antibodies to AQP1, AQP2 and AQP3 were used in conjunction with immunohistochemistry, immunoblotting and quantitative flow cytometry to determine if AQP1, AQP2 and AQP3 are expressed in equine articular chondrocytes. Our studies show that AQP1 and AQP3 are expressed by chondrocytes in articular cartilage in situ and in isolated chondrocytes. We found no evidence for expression of AQP2, the vasopressin-regulated water channel in chondrocytes. AQP1 and AQP3 may be involved in the transport of water and small solutes and osmotically active metabolites across the chondrocyte plasma membrane during volume regulatory behaviour. AQP1 may be involved in transporting metabolic water. AQP3 may participate in the transport of glycerol and structurally related molecules.
Publication Date: 2004-08-11 PubMed ID: 15301762DOI: 10.1016/j.tvjl.2003.08.001Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article is about exploring the presence of Aquaporin water channels AQP1 and AQP3 in equine articular chondrocytes which have a significant role in regulating water and solute content. The researchers found that chondrocytes expressed these channels, thereby potentially influencing their anabolic and catabolic functions.
Background to the Research
- Chondrocytes, the cells in articular cartilage, exist in a unique and highly variable ionic and osmotic environment in the extracellular matrix.
- Any alterations to this environment affect the volume and ionic content of these cells. Consequently, this influences the synthesis and degradation rate of extracellular matrix macromolecules.
- The research focuses on the significance of Aquaporin (AQP) water channels as they effectively influence the water content of the cells.
Objective of the Research
- The purpose of this research was to identify whether AQP water channels were present in chondrocytes from equine articular cartilage.
- Specifically, the researchers investigated the presence of AQP1, AQP2, and AQP3 using techniques such as immunohistochemistry, immunoblotting, and quantitative flow cytometry.
Findings of the Research
- The researchers found that AQP1 and AQP3 were present in chondrocytes in the articular cartilage as well as in isolated chondrocytes.
- No evidence was found to prove the presence of AQP2, which is a vasopressin-regulated water channel, in chondrocytes.
- It is believed that AQP1 and AQP3 may have a role in the transportation of water, small solutes, and osmotically active metabolites across the plasma membrane of chondrocytes during volume regulatory behavior.
- AQP1 might be specifically involved in transporting metabolic water, while AQP3 might play a part in the transportation of glycerol and structurally related molecules.
Cite This Article
APA
Mobasheri A, Trujillo E, Bell S, Carter SD, Clegg PD, Martín-Vasallo P, Marples D.
(2004).
Aquaporin water channels AQP1 and AQP3, are expressed in equine articular chondrocytes.
Vet J, 168(2), 143-150.
https://doi.org/10.1016/j.tvjl.2003.08.001 Publication
Researcher Affiliations
- Connective Tissue and Molecular Pathogenesis Research Groups, Faculty of Veterinary Science, University of Liverpool, Liverpool L69 7ZJ, UK. a.mobasheri@liverpool.ac.uk
MeSH Terms
- Animals
- Aquaporins / metabolism
- Blotting, Western / veterinary
- Cartilage, Articular / cytology
- Cartilage, Articular / metabolism
- Chondrocytes / metabolism
- Flow Cytometry / veterinary
- Horses / metabolism
- Immunohistochemistry / veterinary
Citations
This article has been cited 19 times.- Kyung BS, Jung KW, Yeo WJ, Seo HK, Lee YS, Suh DW. Differential regulation of the water channel protein aquaporins in chondrocytes of human knee articular cartilage by aging.. Sci Rep 2021 Oct 14;11(1):20425.
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- da Silva IV, Soveral G. Aquaporins in Immune Cells and Inflammation: New Targets for Drug Development.. Int J Mol Sci 2021 Feb 12;22(4).
- Rim YA, Nam Y, Park N, Lee K, Jung H, Jung SM, Lee J, Ju JH. Characterization of Early-Onset Finger Osteoarthritis-Like Condition Using Patient-Derived Induced Pluripotent Stem Cells.. Cells 2021 Feb 4;10(2).
- Bezamat M, Cunha EJ, Modesto AM, Vieira AR, Taboas JM. Aquaporin locus (12q13.12) might contribute to susceptibility of temporomandibular joint disorder associated with periodontitis.. PLoS One 2020;15(3):e0229245.
- Snuggs JW, Day RE, Bach FC, Conner MT, Bunning RAD, Tryfonidou MA, Le Maitre CL. Aquaporin expression in the human and canine intervertebral disc during maturation and degeneration.. JOR Spine 2019 Mar;2(1):e1049.
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- Garneau AP, Carpentier GA, Marcoux AA, Frenette-Cotton R, Simard CF, Rémus-Borel W, Caron L, Jacob-Wagner M, Noël M, Powell JJ, Bélanger R, Côté F, Isenring P. Aquaporins Mediate Silicon Transport in Humans.. PLoS One 2015;10(8):e0136149.
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- Mobasheri A, Lewis R, Ferreira-Mendes A, Rufino A, Dart C, Barrett-Jolley R. Potassium channels in articular chondrocytes.. Channels (Austin) 2012 Nov-Dec;6(6):416-25.
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