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Home / Equine Research Bank / Front Allergy / Asthmatic Bronchial Matrices Determine the Gene Expression a...
Frontiers in allergy2021; 2; 762026; doi: 10.3389/falgy.2021.762026

Asthmatic Bronchial Matrices Determine the Gene Expression and Behavior of Smooth Muscle Cells in a 3D Culture Model.

Abstract: Asthma is associated with increased deposition and altered phenotype of airway smooth muscle (ASM) cells. However, little is known about the processes responsible for these changes. It has been suggested that alterations of the extracellular matrix (ECM) contribute to the remodeling of ASM cells in asthma. Three-dimensional matrices allow the study of complex cellular responses to different stimuli in a close-to-natural environment. Thus, we investigated the ultrastructural and genic variations of ASM cells cultured on acellular asthmatic and control bronchial matrices. We studied horses, as they spontaneously develop a human asthma-like condition (heaves) with similarities to chronic pulmonary changes observed in human asthma. Primary bronchial ASM cells from asthmatic ( = 3) and control ( = 3) horses were cultured on decellularized bronchi from control ( = 3) and asthmatic ( = 3) horses. Each cell lineage was used to recellularize six different bronchi for 41 days. Histomorphometry on HEPS-stained-recellularized matrices revealed an increased ASM cell number in the control cell/control matrix ( = 0.02) and asthmatic cell/control matrix group ( = 0.04) compared with the asthmatic cell/asthmatic matrix group. Scan electron microscopy revealed a cell invasion of the ECM. While ASM cells showed high adhesion and proliferation processes on the control ECM, the presence of senescent cells and cellular debris in the asthmatic ECM with control or asthmatic ASM cells suggested cell death. When comparing asthmatic with control cell/matrix combinations by targeted next generation sequencing, only ( = 0.04), ( = 0.009), ( = 0.02), and ( = 0.001) were differentially expressed out of a 70-gene pool previously associated with smooth muscle remodeling. To our knowledge, this is the first attempt to evaluate the effects of asthmatic ECM on an ASM cell phenotype using a biological bronchial matrix. Our results indicate that bronchial ECM health status contributes to ASM cell gene expression and, possibly, its survival.
Copyright © 2021 Ben Hamouda, Miglino, de Sá Schiavo Matias, Beauchamp and Lavoie.
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Publication Date: 2021-11-26 PubMed ID: 35387054PubMed Central: PMC8974673DOI: 10.3389/falgy.2021.762026Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explored how different environments affect the expression of genes in airway smooth muscle (ASM) cells, which play a pivotal role in asthma. The research used a 3D model to investigate how the gene expression and behavior of NIH 3T3 fibroblasts are guided by asthmatic bronchial matrices. Specifically, it compared how ASM cells grow and behave when they are cultured on acellular matrices derived from the bronchi of asthmatic and non-asthmatic control horses.

Methodology of the Research

  • The researchers studied horses, a choice dictated by the species’ natural susceptibility to developing a condition called heaves, which closely mirrors human asthma in its chronic pulmonary changes. Primary bronchial ASM cells were taken from both asthmatic and non-asthmatic horses.
  • These cells were then cultured on decellularized bronchi taken from both asthmatic and non-asthmatic horses, thus creating different experimental groups.
  • Each cell lineage was used to recellularize six different bronchi for a period of 41 days, which were then analyzed.

Findings of the Study

  • The study revealed that there was a greater number of ASM cells in the control cell/control matrix and asthmatic cell/control matrix groups compared to the asthmatic cell/asthmatic matrix group. The finding points to something about the asthmatic matrix that impacts negatively on cell proliferation.
  • Using scanning electron microscopy, the researchers discovered that the ASM cells had invaded the extracellular matrix (ECM). This could have significant implications for understanding how ASM cells interact with their surroundings.
  • In control ECM environments, the ASM cells exhibited high adhesion and proliferation rates. However, in the asthmatic ECM, there was evidence of senescent cells and cellular debris, suggesting cell death.
  • When the researchers compared the asthmatic and control cell/matrix combinations using targeted next-generation sequencing to track gene expression, four genes were differentially expressed out of a gene pool associated with smooth muscle remodeling—out of those four genes, three genes were highly expressed in asthmatic environments.

Implications of the Study

  • This study is the first of its kind to investigate the influence of the asthmatic ECM on an ASM cell phenotype using a biological bronchial matrix.
  • The findings suggest that the health status of the bronchial ECM could play a significant role in ASM cell gene expression, as well as in its survival, thereby bringing new insights into the pathogenesis of asthma.
  • This knowledge might contribute to the development of more effective treatments for asthma in the future.

Cite This Article

APA
Ben Hamouda S, Miglino MA, de Sá Schiavo Matias G, Beauchamp G, Lavoie JP. (2021). Asthmatic Bronchial Matrices Determine the Gene Expression and Behavior of Smooth Muscle Cells in a 3D Culture Model. Front Allergy, 2, 762026. https://doi.org/10.3389/falgy.2021.762026

Publication

Frontiers in allergy
ISSN: 2673-6101
NlmUniqueID: 9918227355906676
Country: Switzerland
Language: English
Volume: 2
Pages: 762026
PII: 762026

Researcher Affiliations

Ben Hamouda, Selma
  • Faculty of Veterinary Medicine, Université de Montréal, Q City, QC, Canada.
Miglino, Maria Angélica
  • School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo, Brazil.
de Sá Schiavo Matias, Gustavo
  • School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo, Brazil.
Beauchamp, Guy
  • Faculty of Veterinary Medicine, Université de Montréal, Q City, QC, Canada.
Lavoie, Jean-Pierre
  • Faculty of Veterinary Medicine, Université de Montréal, Q City, QC, Canada.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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