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Home / Equine Research Bank / Vet World / Biochemical components of seminal plasma and their correlati...
Veterinary world2017; 10(2); 214-220; doi: 10.14202/vetworld.2017.214-220

Biochemical components of seminal plasma and their correlation to the fresh seminal characteristics in Marwari stallions and Poitou jacks.

Abstract: To investigate various biochemical components of seminal plasma in Marwari stallions and Poitou Jacks and to find out their correlation with that of the seminal characteristics. Methods: In this study, semen was collected from six Marwari stallions and six Poitou jacks aged from 4 to 6 years and with known fertility status. The semen collection from the stallions were collected during the breeding season, i.e., between the months of April and June. From the collected semen ejaculates, we estimated the values of some biochemical components, viz., total protein content, total lipid content, and enzymes such as glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), alkaline phosphatase (ALP), acid phosphatase (ACP), and lactate dehydrogenase (LDH) as well as concentrations of glucose, cholesterol, total calcium (Ca), and phosphorus (P) and correlations among different seminal parameters were statistically examined using the Pearson correlation coefficient. Results: In this study, we found positive correlations between semen volume as well as sperm concentration and GOT, GPT, ALP and ACP for both the group stallions. Significant correlation between motility and glucose, GOT and GPT could be an indication for their role metabolism and protection against free radicals to the spermatozoa. Conclusions: Based on the results, it is concluded that there is a positive correlation between some biochemical values such as glucose, Ca, ALP, and LDH and seminal parameters which play a key role in capacitation and onward movement of the spermatozoa.
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Publication Date: 2017-02-18 PubMed ID: 28344405PubMed Central: PMC5352847DOI: 10.14202/vetworld.2017.214-220Google Scholar: Lookup
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Summary

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The researchers studied the biochemical components in the seminal plasma of two different horse breeds to understand their relationship with key characteristics of fresh semen.

Methods

  • The subjects of the study were six Marwari stallions and six Poitou jacks, all between 4 to 6 years of age and already with a known fertility status.
  • Semen samples were collected only during the breeding season that spans from April to June.
  • The researchers considered various biochemical components of seminal plasma for estimation. These included total protein and lipid content, enzymes including glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), alkaline phosphatase (ALP), acid phosphatase (ACP) and lactate dehydrogenase (LDH).
  • Other components like glucose, cholesterol, total calcium (Ca), and phosphorus (P) were also measured in the collected samples.
  • The researchers then statistically analyzed the relationship between the biochemical components and the basic seminal parameters by using the Pearson correlation coefficient.

Results

  • The volume of semen and sperm concentration were found to have positive correlations with the presence of enzymes GOT, GPT, ALP, and ACP in both breeds of horses.
  • A significant relationship was identified between sperm motility and glucose levels, and between motility and the enzymes GOT and GPT. The researchers interpret this correlation as these components possibly playing roles in metabolism and protection against free radicals in sperm cells.

Conclusions

  • The study concludes that there is a positive correlation between key biochemical values (glucose, Ca, ALP, and LDH) and seminal parameters. This relationship could be significant in understanding the process of capacitation and sperm cell movement, and thus fertility in these breeds of horses.

Cite This Article

APA
Talluri TR, Mal G, Ravi SK. (2017). Biochemical components of seminal plasma and their correlation to the fresh seminal characteristics in Marwari stallions and Poitou jacks. Vet World, 10(2), 214-220. https://doi.org/10.14202/vetworld.2017.214-220

Publication

Veterinary world
ISSN: 0972-8988
NlmUniqueID: 101504872
Country: India
Language: English
Volume: 10
Issue: 2
Pages: 214-220

Researcher Affiliations

Talluri, Thirumala Rao
  • Department of Animal Reproduction, Equine Production Campus, Indian Council of Agricultural Research-National Research Centre on Equines, Bikaner, Rajasthan-334001.
Mal, Gorakh
  • Department of Biochemistry, Biochemistry Laboratory, Indian Council of Agricultural Research - Indian Veterinary Research Institute, Palampur - 176 061, Himachal Pradesh, India.
Ravi, Sanjay Kumar
  • Department of Animal Reproduction, Equine Production Campus, Indian Council of Agricultural Research-National Research Centre on Equines, Bikaner, Rajasthan-334001.

References

This article includes 38 references
  1. Juyena N. S, Stelletta C. Seminal plasma: An essential attribute to spermatozoa.. J. Androl. 2012;33:536–551.
    pubmed: 22016346
  2. Asadpour R. Relationship between mineral composition of seminal plasma and semen quality in various ram breeds.. Acta Sci. Vet. 2012;40:1027.
  3. Tvrda E, Sikeli P, Lukacova J, Massanyi P, Lukac N. Mineral nutrients and male fertility.. J. Microbiol. Biotechnol. Food Sci. 2013;3:1–14.
  4. El-Bishbishy H. A, Aly H. A, El-Shafey M. Lipoic acid mitigates bisphenol A-induced testicular mitochondrial toxicity in rats.. Environ. Health. 2013;29(10):875–887.
    pubmed: 22623521
  5. Bucci D, Giaretta E, Spinaci M, Rizzato G, Isani G. Characterization of alkaline phosphatase activity in seminal plasma and in fresh and frozen-thawed stallion spermatozoa.. Theriogenology 2016;85:288–295.
    pubmed: 26433714
  6. Duan C, Goldberg E. Inhibition of lactate dehydrogenase C4 (LDHC4) blocks capacitation of mouse sperm in vitro.. Cytogenet. Genome. Res. 2003;103(3-4):352–359.
    pubmed: 15051959
  7. Eghbali M, Alavi-Shoushtari S. M, Asri-Rezaei S, Ansari M. H. K. Effects of the seminal plasm iron and lead content on semen quality of water buffalo (Bubalus bubalis) bulls.. Vet. Res. Forum. 2010;3:142–148.
  8. Pesch S, Bergmann M, Bostedt H. Determination of some enzymes and macro and microelements in stallion seminal plasma and their correlations to semen quality.. Theriogenology 2006;66(2):307–313.
    pubmed: 16413936
  9. Intasqui P, Camargo M, Antoniassi M. P, Cedenho A. P, Carvalho M. Association between the seminal plasma proteome and sperm functional traits.. Fertil. Steril. 2016;105:617–628.
    pubmed: 26621572
  10. Knazicka Z, Lukacova J, Gren A, Formicki G, Massanyi P, Lukac N. Relationship between level of copper in bovine seminal plasma and spermatozoa motility.. J. Microbiol. Biotechnol. Food Sci. 2013;2:1351–1362.
  11. Argov-Argaman N, Mahgrefthe K, Zeron Y, Roth Z. Variation in lipid profiles within semen compartment - The bovine model of aging.. Theriogenology 2013;80:712–721.
    pubmed: 23830232
  12. Talluri T. R, Arangasamy A, Ravi S. K, Pal Y. Hypo-osmotic swelling test for quality evaluation of fresh and frozen semen quality in horses.. Indian Vet. J. 2012;89(11):68–70.
  13. Macanovic B, Vucetic M, Jankovic A, Stancic A, Buzadzic B, Garalejic E, Korac A, Korac B, Otasevic V. Correlation between sperm parameters and protein expression of antioxidative defense enzymes in seminal plasma: A pilot study.. Dis. Markers. 2015;2015:436236.
    pmc: PMC4323057pubmed: 25691806
  14. Arangasamy A, Tandon S. N. Evaluation of frozen semen characteristics of Marwari stallions and Poitou Jacks.. Indian Vet. J. 2008;11:1168–1169.
  15. Pal Y, Legha R. A, Tandon S. N. Comparative assessment of seminal characteristics of horse and donkey stallions.. Indian J. Anim. Sci. 2009;79(10):1028–1029.
  16. Dogan I, Polat U, Nur Z. Correlations between seminal plasma enzyme activities and semen parameters in seminal fluid of Arabian horses.. Iran. J. Vet. Res. 2009;10(2):119–124.
  17. Aguiar G. V, Van Tilburg M. F, Catunda A. G. V, Celes C. K. S, Lima I. C. S, Campos A. C. N, Moura A. A. A, Araujo A. A. Sperm parameters and biochemical components of goat seminal plasma in the rainy and dry seasons in the Brazilian Northeast: The season’s influence on the cooling of semen.. Arq. Bras. Med. Vet. Zootec. 2013;65(1):6–12.
  18. Mahsud T, Jamil H, Qureshi Z. I, Asi M. N, Lodhi L. A, Waqas M. S. Semen quality parameters and selected bio-chemical constituents level in plasma of Lohirams.. Small Rumin. Res. 2013;113:175–178.
  19. Lopez Rodriguez A, Rijsselaere T, Beek J, Vyt P, Van Soom A, Maes D. Boar seminal plasma components and their relation with semen quality.. Syst. Biol Reprod. Med. 2013;59:5–12.
    pubmed: 23083319
  20. Hinton B. T, Lan Z. J, Rudolph D. B, Labus J. C, Lye R. J. Testicular regulation of epididymal gene expression.. J. Reprod. Fertil. Suppl. 1998;53:47–57.
    pubmed: 10645265
  21. Coleman J. E. Structure and mechanism of alkaline phosphatase.. Annu. Rev. Biophys. Biomol. Struct. 1992;21:441–483.
    pubmed: 1525473
  22. Bucci D, Giaretta E, Spinaci M, Rizzato G, Isani G, Mislei B, Mari G, Tamanini C, Galeati G. Characterization of alkaline phosphatase activity in seminal plasma and in fresh and frozen-thawed stallion spermatozoa.. Theriogenology 2016;85:288–295.
    pubmed: 26433714
  23. Turner R. M, McDonnell S. M. Alkaline phosphatase in stallion semen: Characterization and clinical applications.. Theriogenology 2003;60(1):1–10.
    pubmed: 12620574
  24. Schafer-Somi S, Frohlich T, Schwendenwein I. Measurement of alkaline phosphatase in canine seminal plasma - An update.. Reprod. Domest. Anim. 2013;48:e10–12.
    pubmed: 22524622
  25. Kiso W. K, Selvaraj V, Nagashima J, Asano A, Brown J. L, Schmitt D. L, Leszyk J, Travis A. J, Pukazhenthi B. S. Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.. PLoS One 2013;8:e71033.
    pmc: PMC3745378pubmed: 23976974
  26. Stornelli A, Arauz M, Baschard H, de la Sota R. L. Unilateral and bilateral vasectomy in the dog: Alkaline phosphatase as an indicator of tubular patency.. Reprod. Domest. Anim. 2003;38:1–4.
    pubmed: 12535322
  27. Kashir J, Jones C, Child T, Williams S. A, Coward K. Viability assessment for artificial gametes: The need for biomarkers of functional competency.. Biol. Reprod. 2012;87(5):1–11.
    pubmed: 23018181
  28. Kurien M, Katheresan D, Selvaraju M, Pattabiraman S. Macroscopic, microscopic and bio-chemical characteristics of fresh dog semen.. Indian J. Anim. Reprod. 2015;33(1):18–20.
  29. Feng R. X, Lu J. C, Zhang H. Y, Lü N. Q. A pilot comparative study of 26 biochemical markers in seminal plasma and serum in infertile men.. BioMed Res. Int. 2015;2015:1–7.
    pmc: PMC4619868pubmed: 26539526
  30. Tanaka M, Kishi Y, Takanezawa Y, Kakehi Y, Aoki J, Arai H. Prostatic acid phosphatase degrades lysophosphatidic acid in seminal plasma.. FEBS. Lett. 2004;571:197–204.
    pubmed: 15280042
  31. Tappel A. Lysosomal and prostasomal hydrolytic enzymes and redox processes and initiation of prostate cancer.. Med. Hypotheses. 2005;64:1170–1172.
    pubmed: 15823710
  32. Alkafafy M, Ebada S, Rashed R, Attia H. Comparative morphometric and glycohistochemical studies on the epididymal duct in the donkey (Equus asinus) and dromedary camel (Camelus dromedarius). Acta Histochem. 2012;114:434–447.
    pubmed: 21906788
  33. Knecht D, Środoń S, Duziński K. The influence of boar breed and season on semen parameters.. S. Afr. J. Anim. Sci. 2014;44(1):1–9.
  34. Kamp G, Busselmann G, Lauterwein J. Spermatozoa: Models for studying regulatory aspects of energy metabolism.. Experientia 1996;52:487–494.
    pubmed: 8641386
  35. Mahamud M. A, Onu J. E, Shehu S. A, Umar M. A, Bello A, Danmaigoro A. Cryptorchidism in mammals: A review.. Glob. J. Anim. Sci. Res. 2015;3(1):128–135.
  36. Baker M. A, Hetherington L, Ecroyd H, Roman S. D, Aitken R. J. Analysis of the mechanisms by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation.. J. Cell Sci. 2004;117:211–222.
    pubmed: 14676274
  37. Marquez B, Suarez S. S. Different signaling pathways in bovine sperm regulate capacitation and hyper activation.. Biol. Reprod. 2004;70:1626–1633.
    pubmed: 14766720
  38. Kwon W. S, Park Y. J, El-Mohamed S. A, Pang M. G. Voltage-dependent anion channels are a key factor of male fertility.. Fertil. Steril. 2013;99:354–361.
    pubmed: 23062735
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