Carboxymethylated phosphatidylethanolamine in mitochondrial membranes of mammals–evidence for intracellular lipid glycoxidation.
Abstract: The non-enzymatic modification of aminophospholipids with lipoperoxidation-derived aldehydes and glycoxidation-derived products have been reported previously. However, it remains unknown whether intracellular membranes are damaged by these glycoxidation-derived products. To investigate this issue, we tested whether aminophospholipids from mitochondrial membranes are damaged by glycoxidative stress the mitochondrion being identified as the major site of reactive-species production in the cell. We have used a selected-ion-monitoring/gas-chromatography/mass-spectrometry assay for carboxymethylethanolamine (CM-Etn) detection, and provide evidence for the presence of CM-Etn in mitochondrial phospholipids. Further, as a physiological approach to evaluate the influence of mitochondrial oxidative stress in CM-Etn formation, we also present the comparative levels of CM-Etn in mitochondrial membranes of ten mammalian species ranging in maximum life-span from 3.5 years to 100, since the rate of mitochondrial reactive-oxygen-species production is inversely correlated to the maximum life-span. Our results show that CM-Etn levels correlate in a logarithmic fashion with the maximum-life-span [[CM-Etn] = 0.51 + 0.50 x', where x' = log(maximum-life-span); r = 0.81, P < 0.004]. The data demonstrate the intracellular occurrence of glycoxidative processes affecting membrane lipids. Moreover, these data show that longer-lived mammals contain higher levels of CM-Etn in mitochondrial membrane aminophospholipids. This trend could result from differences in rates of CM-Etn accumulation and/or phospholipid turnover.
Publication Date: 1998-09-17 PubMed ID: 9738908DOI: 10.1046/j.1432-1327.1998.2550685.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research investigated the impact of specific oxidative stress products on the mitochondrial membranes of various mammalian species, and how it relates to their lifespan. The results indicated that the longer a species lives, the higher their levels of carboxymethylethanolamine in mitochondrial membrane aminophospholipids, implying the influence of oxidative stresses on cellular aging.
Research Objective and Methodology
- The main focus of the study was to examine the effects of specific glycoxidation by-products on the mitochondrial membranes in various mammalian cells. This aimed to explore a possible link with aging.
- The researchers picked up from previous studies that reported non-enzymatic modification of aminophospholipids with lipoperoxidation-derived aldehydes and glycoxidation-derived products but left the impact on intracellular membranes unknown.
- In order to determine whether intracellular membranes are damaged by these glycoxidation by-products, the team investigated mitochondrial aminophospholipids, as mitochondria are notorious for being the primary reactive-species production site within a cell.
- The team used a selected-ion-monitoring/gas-chromatography/mass-spectrometry assay to detect carboxymethylethanolamine (CM-Etn) and found evidence of its presence in mitochondrial phospholipids.
Comparative Analysis Across Species
- The researchers compared CM-Etn levels in mitochondrial membranes across ten mammalian species with varying lifespans, ranging from 3.5 to 100 years.
- The objective was to test the hypothesis that the rate of mitochondrial reactive-oxygen-species production is inversely correlated with maximum lifespan. This was done by considering that oxidative stress in mitochondria influences CM-Etn formation.
- The results showed a logarithmic correlation between CM-Etn levels and maximum lifespan, suggesting that longer-lived mammals have higher levels of CM-Etn in their mitochondrial membrane aminophospholipids.
Significance of Findings
- The findings demonstrate the occurrence of intracellular glycoxidative processes that affect membrane lipids. This is significant as it offers evidence of the damage caused by glycoxidation by-products on intracellular membranes.
- Moreover, the study also proves that longer-lived mammals contain higher levels of CM-Etn in their mitochondrial membrane aminophospholipids, linking these processes to the aging process. This could either be due to differences in the rate of CM-Etn accumulation or phospholipid turnover.
Cite This Article
APA
Pamplona R, Requena JR, Portero-Otín M, Prat J, Thorpe SR, Bellmunt MJ.
(1998).
Carboxymethylated phosphatidylethanolamine in mitochondrial membranes of mammals–evidence for intracellular lipid glycoxidation.
Eur J Biochem, 255(3), 685-689.
https://doi.org/10.1046/j.1432-1327.1998.2550685.x Publication
Researcher Affiliations
- Departament de Ciències Mèdiques Bàsiques, Universitat de Lleida, Spain.
MeSH Terms
- Animals
- Cattle
- Cricetinae
- Dogs
- Guinea Pigs
- Horses
- Humans
- Membrane Lipids / analysis
- Mice
- Mitochondria / chemistry
- Phosphatidylethanolamines / analysis
- Phosphatidylethanolamines / chemistry
- Rabbits
- Sheep
- Swine
Grant Funding
- G11472 / PHS HHS
Citations
This article has been cited 10 times.- Ito G, Tatara Y, Itoh K, Yamada M, Yamashita T, Sakamoto K, Nozaki T, Ishida K, Wake Y, Kaneko T, Fukuda T, Sugano E, Tomita H, Ozaki T. Novel dicarbonyl metabolic pathway via mitochondrial ES1 possessing glyoxalase III activity.. BBA Adv 2023;3:100092.
- Smith AJ, Advani J, Brock DC, Nellissery J, Gumerson J, Dong L, Aravind L, Kennedy B, Swaroop A. GATD3A, a mitochondrial deglycase with evolutionary origins from gammaproteobacteria, restricts the formation of advanced glycation end products.. BMC Biol 2022 Mar 21;20(1):68.
- Altomare A, Baron G, Gianazza E, Banfi C, Carini M, Aldini G. Lipid peroxidation derived reactive carbonyl species in free and conjugated forms as an index of lipid peroxidation: limits and perspectives.. Redox Biol 2021 Jun;42:101899.
- Solís-Calero C, Ortega-Castro J, Frau J, Muñoz F. Nonenzymatic Reactions above Phospholipid Surfaces of Biological Membranes: Reactivity of Phospholipids and Their Oxidation Derivatives.. Oxid Med Cell Longev 2015;2015:319505.
- Wang H, Guo J, West XZ, Bid HK, Lu L, Hong L, Jang GF, Zhang L, Crabb JW, Linetsky M, Salomon RG. Detection and biological activities of carboxyethylpyrrole ethanolamine phospholipids (CEP-EPs).. Chem Res Toxicol 2014 Dec 15;27(12):2015-22.
- Solís-Calero C, Ortega-Castro J, Hernández-Laguna A, Muñoz F. DFT study of the mechanism of the reaction of aminoguanidine with methylglyoxal.. J Mol Model 2014 Apr;20(4):2202.
- Naudí A, Jové M, Ayala V, Cabré R, Portero-Otín M, Pamplona R. Non-enzymatic modification of aminophospholipids by carbonyl-amine reactions.. Int J Mol Sci 2013 Feb 5;14(2):3285-313.
- Pun PB, Murphy MP. Pathological significance of mitochondrial glycation.. Int J Cell Biol 2012;2012:843505.
- González O, Tobia C, Ebersole J, Novak MJ. Caloric restriction and chronic inflammatory diseases.. Oral Dis 2012 Jan;18(1):16-31.
- Shoji N, Nakagawa K, Asai A, Fujita I, Hashiura A, Nakajima Y, Oikawa S, Miyazawa T. LC-MS/MS analysis of carboxymethylated and carboxyethylated phosphatidylethanolamines in human erythrocytes and blood plasma.. J Lipid Res 2010 Aug;51(8):2445-53.
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