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Home / Equine Research Bank / BMC Vet Res / Changes of microbial and metabolome of the equine hindgut du...
BMC veterinary research2021; 17(1); 11; doi: 10.1186/s12917-020-02686-9

Changes of microbial and metabolome of the equine hindgut during oligofructose-induced laminitis.

Abstract: Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction. However, the pathologic mechanism remains unclear. In this study we aimed to investigate changes of the gut microbiota and metabolomics in oligofructose-induced laminitis of horses. Results: Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum. Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera. In addition, the metabolome analysis revealed that treatment with oligofructose decreased 84 metabolites while 53 metabolites increased, such as dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic acid, and asparagine. Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses. Furthermore, correlation analysis between gut microbiota and metabolites indicated that Lactobacillus and/or Megasphaera were positively associated with the dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic acid, and asparagine. Conclusions: These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
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Publication Date: 2021-01-06 PubMed ID: 33407409PubMed Central: PMC7789226DOI: 10.1186/s12917-020-02686-9Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents a study focusing on changes in gut microbiota and metabolites induced by oligofructose, a known inflammation-inducer, which can lead to a severe disease in horses called laminitis. The study reveals how these changes affect the health of horses and offers clues for potential prevention strategies targeting the horse’s microbiota and metabolites.

Objective and Methodology

  • The aim of the research was to investigate changes in gut microbiota and metabolites during oligofructose-induced laminitis in horses.
  • Inducing laminitis chemically with oligofructose allowed researchers to study physiological changes associated with the disease.
  • The sample group consisted of healthy horses, some of which were subsequently treated with oligofructose. Their feces and serum were studied for changes in their microbiota and metabolites.

Results

  • Horses treated with oligofructose had lower fecal pH levels, indicating a more acidic gut environment, which could potentially disrupt the gut microbiota.
  • Increased levels of lactic acid, histamine, and Lipopolysaccharide (LPS) in serum were observed, which could be indicative of an inflammatory response.
  • The treatment altered the hindgut bacterial community, with an increase in the relative abundance of the bacteria Lactobacillus and Megasphaera.
  • Metabolomic analysis revealed a decrease in 84 metabolites and an increase in 53 metabolites, such as dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic acid, and asparagine.
  • Changes were observed in various metabolic pathways including aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism.

Connections and Conclusion

  • Correlation analysis was carried out between gut microbiota and metabolites, showing that Lactobacillus and/or Megasphaera were positively associated with specific metabolites, which were either increased or decreased during laminitis.
  • This research demonstrates a connection between gut microbiota disturbance and changes in metabolic products during the development of equine laminitis.
  • The findiings may provide potential biomarkers and opens new avenues to understand the mechanism of the disease, as well as devise preventive strategies targeting microbiota and metabolites for laminitis in horses.

Cite This Article

APA
Tuniyazi M, He J, Guo J, Li S, Zhang N, Hu X, Fu Y. (2021). Changes of microbial and metabolome of the equine hindgut during oligofructose-induced laminitis. BMC Vet Res, 17(1), 11. https://doi.org/10.1186/s12917-020-02686-9

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 17
Issue: 1
Pages: 11

Researcher Affiliations

Tuniyazi, Maimaiti
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China.
He, Junying
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China.
Guo, Jian
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China.
Li, Shuang
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China.
Zhang, Naisheng
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China.
Hu, Xiaoyu
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China. huxiaoyu@jlu.edu.cn.
Fu, Yunhe
  • Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China. fuyunhesky@163.com.

MeSH Terms

  • Animals
  • Bacteria / classification
  • Bacteria / metabolism
  • Female
  • Foot Diseases / chemically induced
  • Foot Diseases / metabolism
  • Foot Diseases / microbiology
  • Foot Diseases / veterinary
  • Gastrointestinal Microbiome
  • Histamine / blood
  • Hoof and Claw
  • Horse Diseases / chemically induced
  • Horse Diseases / metabolism
  • Horse Diseases / microbiology
  • Horses
  • Inflammation / metabolism
  • Inflammation / microbiology
  • Inflammation / veterinary
  • Lactic Acid / blood
  • Lipopolysaccharides / blood
  • Male
  • Metabolome
  • Oligosaccharides
  • Ultrasonography, Doppler / veterinary

Grant Funding

  • 31472248 / National Natural Science Foundation of China
  • 31772812 / National Natural Science Foundation of China

Conflict of Interest Statement

None of the authors have a financial interest in any of the products, devices, or Materials mentioned in this manuscript. The authors declare that they have no conflicts of interest.

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