Characterization, genetic and physical mapping analysis of 36 horse plasmid and cosmid-derived microsatellites.
Abstract: Thirty-six new horse microsatellites (11 from plasmid libraries and 25 from a cosmid library) were isolated and characterized on a panel of four horse breeds. Thirty were found to be polymorphic with heterozygosity levels ranging between 0.20 and 0.87. Twenty-two of the cosmids were physically mapped to R-banded single horse Chromosomes (Chrs) 1, 3, 4, 9, 11, 12, 13, 15, 18, 19, 21, 22, 23 and three to pericentromeric regions. Furthermore, linkage analysis between a selection of 42 DNA markers, including those presented in this study, and 16 conventional markers of the horse hemotype was performed on six paternal half-sib horse families. Five linkage groups were detected, of which four were assigned to Chr 10, 11, 15, and 18. This work increased by one-third the number of published polymorphic DNA markers suitable for horse mapping and approximately doubled the number of known linkage groups. Our cosmids labeled 14 out of the 31 horse autosomes. Moreover, the physical anchoring of part of these markers will orient linkage and synteny groups on the chromosomes and will contribute to their assignment.
Publication Date: 1997-10-08 PubMed ID: 9321468DOI: 10.1007/s003359900558Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study discusses the isolation and examination of 36 new horse microsatellites from horse genetic libraries. The study found a diverse range of heterozygosity among these microsatellites and physically mapped them to specific horse chromosomes. This work significantly increased the number of known polymorphic DNA markers for horse mapping and established associations between many of these markers and specific horse chromosomes.
Research Goals and Methods
- The aim of this study was to isolate and examine new horse microsatellites from plasmid and cosmid libraries. These libraries contain segments of horse DNA.
- The researchers explored the genetic diversity of the isolated microsatellites by determining their levels of heterozygosity, which measures the genetic variation at a particular location in the DNA.
- They also physically mapped the microsatellites to horse chromosomes, which can help assign these markers to specific genomic locations for further functional analyses.
Research Findings
- The researchers isolated 36 new horse microsatellites from the libraries and found them to be diverse, with levels of heterozygosity ranging between 0.20 and 0.87.
- The physical mapping exercise allowed the researchers to assign the Cosmids to 14 different horse chromosomes and to three pericentromeric regions, which are regions near the center of chromosomes.
- The researchers conducted linkage analysis between various DNA markers and conventional markers of the horse genotype on six horse families that share the same father. They found five DNA linkage groups and assigned them to specific horse chromosomes.
Significance of the Research
- The research significantly increased the number of known polymorphic DNA markers suitable for horse mapping by a third.
- It also nearly doubled the number of known linkage groups, which are groups of genes on a chromosome that tend to be inherited together.
- The physical mapping of the microsatellites contributes to the orientation of linkage groups on the chromosomes, aiding in future genetic studies for horses.
- The research has potential implications for understanding genetic disorders, breeding, and the evolution of horses.
Cite This Article
APA
Godard S, Vaiman D, Oustry A, Nocart M, Bertaud M, Guzylack S, Mériaux JC, Cribiu EP, Guérin G.
(1997).
Characterization, genetic and physical mapping analysis of 36 horse plasmid and cosmid-derived microsatellites.
Mamm Genome, 8(10), 745-750.
https://doi.org/10.1007/s003359900558 Publication
Researcher Affiliations
- Laboratoire de Génétique biochimique et de Cytogénétique, INRA-Centre de Recherches de Jouy, Jouy-en-Josas, France.
MeSH Terms
- Animals
- Chromosome Banding
- Chromosome Mapping
- Chromosomes / genetics
- Cosmids
- DNA / isolation & purification
- Gene Library
- Genetic Linkage
- Horses / genetics
- In Situ Hybridization, Fluorescence
- Microsatellite Repeats
- Phenotype
- Plasmids
Citations
This article has been cited 6 times.- Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence. Chromosome Res 2008;16(1):109-27.
- Brinkmeyer-Langford C, Raudsepp T, Lee EJ, Goh G, Schäffer AA, Agarwala R, Wagner ML, Tozaki T, Skow LC, Womack JE, Mickelson JR, Chowdhary BP. A high-resolution physical map of equine homologs of HSA19 shows divergent evolution compared with other mammals. Mamm Genome 2005 Aug;16(8):631-49.
- Lindgren G, Breen M, Godard S, Bowling A, Murray J, Scavone M, Skow L, Sandberg K, Guérin G, Binns M, Ellegren H. Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis. Chromosome Res 2001;9(1):53-9.
- Shiue Y-L, Millon LV, Skow LC, Honeycutt D, Murray JD, Bowling AT. Synteny and regional marker order assignment of 26 type I and microsatellite markers to the horse X- and Y-chromosomes. Chromosome Res 2000;8(1):45-55.
- Caetano AR, Shiue YL, Lyons LA, O'Brien SJ, Laughlin TF, Bowling AT, Murray JD. A comparative gene map of the horse (Equus caballus). Genome Res 1999 Dec;9(12):1239-49.
- Lindgren G, Sandberg K, Persson H, Marklund S, Breen M, Sandgren B, Carlstén J, Ellegren H. A primary male autosomal linkage map of the horse genome. Genome Res 1998 Sep;8(9):951-66.
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