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Home / Equine Research Bank / Immunology / Characterization of equine E-selectin.
Immunology2001; 103(4); 498-504; doi: 10.1046/j.1365-2567.2001.01262.x

Characterization of equine E-selectin.

Abstract: Expression of E-selectin on activated endothelium is a critical initial step that leads to extravasation of leucocytes during inflammation, yet E-selectin is largely uncharacterized in several animal species including the horse. We have sequenced and compared E-selectin genes derived from activated cultures of purified equine (horse), cervid (black-tailed deer) and ovine (sheep) pulmonary artery endothelial cells (ECs). Phylogenetic and amino acid sequence comparisons indicate that bovine, cervid and ovine E-selectin are similar, whereas human and equine E-selectin are more closely related to each other than to the ruminant molecules. Human E- and P-selectin-specific monoclonal antibodies that also recognize equine E-selectin were identified and used to characterize its expression. Expression of E-selectin was more readily induced by lipopolysaccharide treatment in equine ECs than in human ECs and supported adhesion and activation of neutrophils, consistent with the extreme sensitivity of horses to endotoxaemia and septic shock.
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Publication Date: 2001-09-01 PubMed ID: 11529941PubMed Central: PMC1783268DOI: 10.1046/j.1365-2567.2001.01262.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper discusses the examination and comparison of the E-selectin genes in different species, focusing on the horse’s E-selectin which is shown to be more responsive when exposed to certain bacterial substances, leading to inflammation.

E-Selectin Gene Comparison across Species

  • The study focused on comparing the E-selectin genes in species such as horse, black-tailed deer, sheep, and humans. E-selectin is a protein that plays a critical role in the immune response, helping white blood cells migrate towards the site of inflammation.
  • With the use of phylogenetic and amino acid sequence comparisons, they observed similarity between the E-selectin in bovine, cervid, and ovine, that is, the cows, deer, and sheep.
  • Interestingly, the E-selectin genes in humans and horses were closely related to each other than they were to the ruminant animals.

Role of E-Selectin in Inflammation and Immune Response

  • They further investigated the role of E-selectin in inflammation by using certain monoclonal antibodies that recognize it. Monoclonal antibodies are specialized proteins that can bind to specific cells or proteins, making them useful in identifying and targeting particular substances in a organism.
  • Through this method, the scientists were able to characterize the expression of equine E-selectin, that is, how active it was in the presence of certain stimuli.

Equine Response to Lipopolysaccharide Exposure

  • When treated with lipopolysaccharide, a kind of bacterial substance that often triggers inflammation, the E-selectin in equine cells responded more readily than in human cells. Lipopolysaccharide is a common trigger for inflammation, and can be particularly harmful in larger quantities.
  • The researchers noted that this increased activity led to the adhesion and activation of neutrophils, a type of white blood cell. This heightened immune response aligns with the high sensitivity of horses to conditions like endotoxemia and septic shock, which are caused by bacterial toxins in the bloodstream.

Cite This Article

APA
Hedges JF, Demaula CD, Moore BD, McLaughlin BE, Simon SI, MacLachlan NJ. (2001). Characterization of equine E-selectin. Immunology, 103(4), 498-504. https://doi.org/10.1046/j.1365-2567.2001.01262.x

Publication

Immunology
ISSN: 0019-2805
NlmUniqueID: 0374672
Country: England
Language: English
Volume: 103
Issue: 4
Pages: 498-504

Researcher Affiliations

Hedges, J F
  • Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Demaula, C D
    Moore, B D
      McLaughlin, B E
        Simon, S I
          MacLachlan, N J

            MeSH Terms

            • Amino Acid Sequence
            • Animals
            • Antibodies, Monoclonal / immunology
            • Cell Adhesion / physiology
            • Cell Culture Techniques
            • Cross Reactions
            • Deer / genetics
            • E-Selectin / chemistry
            • E-Selectin / genetics
            • E-Selectin / immunology
            • Endothelium, Vascular / metabolism
            • Horses / genetics
            • Humans
            • Lipopolysaccharides / immunology
            • Molecular Sequence Data
            • Neutrophil Activation / physiology
            • Phylogeny
            • Polymerase Chain Reaction
            • Sheep / genetics
            • Species Specificity

            Grant Funding

            • R01 AI047294 / NIAID NIH HHS
            • AI47294 / NIAID NIH HHS

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