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Home / Equine Research Bank / J Vet Med Sci / Characterization of glycoproteins in equine herpesvirus-1.
The Journal of veterinary medical science2013; 75(10); 1317-1321; doi: 10.1292/jvms.13-0168

Characterization of glycoproteins in equine herpesvirus-1.

Abstract: In this study, we attempted to express twelve glycoproteins of equine herpesvirus-1 (EHV-1) in 293T cells and to characterize these using monoclonal antibodies (MAbs) and horse sera against EHV-1. Expression of glycoprotein B (gB), gC, gD, gG, gI and gp2 was recognized by immunoblot analysis using horse sera, but that of gE, gH, gK, gL, gM and gN was not. Four MAbs recognized gB, four recognized gC and one recognized gp2. Two MAbs against gB cross-reacted with EHV-4. Interestingly, coexpression of gE and gI and gM and gN enhanced their antigenicity. Furthermore, immunoblot analysis of gp2 showed that different molecular masses of gp2 were recognized by the MAb against gp2 and horse sera against EHV-1. In this study, it was demonstrated that at least six glycoproteins were immunogenic to horses, and coexpression of gE and gI and gM and gN was important for enhancement of antigenicity.
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Publication Date: 2013-07-07 PubMed ID: 23748975PubMed Central: PMC3942931DOI: 10.1292/jvms.13-0168Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article primarily explores the expression and characterization of twelve glycoproteins in equine herpesvirus-1, and their response to equine sera and monoclonal antibodies.

Study Methodology and Techniques

  • The researchers attempted to express twelve glycoproteins (gB, gC, gD, gE, gG, gH, gI, gK, gL, gM, gN, and gp2) of equine herpesvirus-1 (EHV-1) in 293T cells.
  • The characterization of these proteins was then conducted using monoclonal antibodies (MAbs) and horse sera against EHV-1.
  • Immunoblot analysis was performed to determine the proteins’ expression and their reactivity with the equine sera.

Research Findings and Observations

  • Out of all the twelve glycoproteins, only the expression of gB, gC, gD, gG, gI, and gp2 was recognized by the horse sera through immunoblot analysis. The remaining six glycoproteins (gE, gH, gK, gL, gM, gN) did not show any detection.
  • The monoclonal antibodies reacted differently with the glycoproteins: Four recognized gB, four recognized gC, and one recognized gp2.
  • Interestingly, two of the monoclonal antibodies that reacted with gB also showed cross-reactivity with equine herpesvirus-4 (EHV-4).
  • Furthermore, the study observed that the coexpression of certain couples of glycoproteins (gE and gI, gM and gN) seemed to enhance their antigenicity, indicating these combinations might be significant for immune response trigger.
  • An unexpected result emerged during the immunoblot analysis of gp2. Different molecular masses of the protein were recognized by the MAb against gp2 and the horse sera against EHV-1.

Conclusion

  • The study concludes that out of the twelve glycoproteins studied, at least six (gB, gC, gD, gG, gI and gp2) showed immunogenicity to horses, suggesting they could trigger the immune response in horses to fight against EHV-1.
  • The enhanced antigenicity during the coexpression of gE and gI, and gM and gN, indicates these combinations might be critical for the virus’s ability to stimulate an immune response.
  • The differential recognition of gp2’s molecular masses by the MAb and horse sera necessitates more detailed investigation.

Cite This Article

APA
Mahmoud HY, Andoh K, Hattori S, Terada Y, Noguchi K, Shimoda H, Maeda K. (2013). Characterization of glycoproteins in equine herpesvirus-1. J Vet Med Sci, 75(10), 1317-1321. https://doi.org/10.1292/jvms.13-0168

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 75
Issue: 10
Pages: 1317-1321

Researcher Affiliations

Mahmoud, Hassan Y A H
  • Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
Andoh, Kiyohiko
    Hattori, Shiho
      Terada, Yutaka
        Noguchi, Keita
          Shimoda, Hiroshi
            Maeda, Ken

              MeSH Terms

              • Animals
              • Antibodies, Monoclonal / immunology
              • Cell Line
              • Female
              • Glycoproteins / genetics
              • Glycoproteins / immunology
              • Herpesviridae Infections / immunology
              • Herpesviridae Infections / veterinary
              • Herpesviridae Infections / virology
              • Herpesvirus 1, Equid / genetics
              • Herpesvirus 1, Equid / immunology
              • Horse Diseases / immunology
              • Horse Diseases / virology
              • Horses
              • Immunoblotting / veterinary
              • Mice
              • Mice, Inbred BALB C
              • Neutralization Tests / veterinary
              • Plasmids / genetics
              • Plasmids / immunology

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              Citations

              This article has been cited 1 times.
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