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Home / Equine Research Bank / Immunogenetics / Characterization of horse (Equus caballus) T-cell receptor b...
Immunogenetics1994; 40(2); 135-144; doi: 10.1007/BF00188177

Characterization of horse (Equus caballus) T-cell receptor beta chain genes.

Abstract: Genes encoding the horse (Equus caballus) T-cell receptor beta chain (TCRB) were cloned and characterized. Of 33 cDNA clones isolated from the mesenteric lymph node, 30 had functionally rearranged gene segments, and three contained germline sequences. Sixteen unique variable segments (TCRBV), 14 joining genes (TCRBJ), and two constant region genes (TCRBC) were identified. Horse TCRBV were grouped into nine families based on similarity to human sequences. TCRBV2 and TCRBV12 were the most commonly represented horse families. Analysis of predicted protein structure revealed the presence of conserved regions similar to those seen in TCRB of other species. A decanucleotide promoter sequence homologous to those found in humans and mice was located in the 5' untranslated region of one horse gene. Germline sequences included the 5' region of the TCRBD2 gene with flanking heptamer/nonamer recombination signals and portions of the TCRBJ2-C2 intron. Southern blot hybridizations demonstrated restriction fragment length polymorphisms at the TCRBC locus among different horse breeds.
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Publication Date: 1994-01-01 PubMed ID: 7913080DOI: 10.1007/BF00188177Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on understanding the genes encoding the horse T-cell receptor beta chain (TCRB). The researchers were able to identify 30 functionally rearranged gene segments, three containing germline sequences, sixteen unique variable segments, and more.

Methodology and Findings

  • The experiment began with the cloning and characterization of genes that encode the T-cell receptor beta chain (TCRB) in horses. Out of the total 33 cDNA clones isolated from the mesenteric lymph node, 30 represented functionally rearranged gene segments while the remaining 3 carried germline sequences.
  • The researchers identified 16 unique variable segments (TCRBV), 14 joining genes (TCRBJ), and 2 constant region genes (TCRBC).
  • The horse TCRBV were categorized into nine families based on similarity to human sequences. TCRBV2 and TCRBV12 were identified as the most represented horse families.
  • The predicted protein structure analysis revealed the existence of conserved regions which are similar to those found in TCRB of other species.
  • The researchers found a decanucleotide promoter sequence similar to those in humans and mice in the 5′ untranslated region of one horse gene. The germline sequences included the 5′ region of the TCRBD2 gene, along with flanking heptamer/nonamer recombination signals and sections of the TCRBJ2-C2 intron.

Important Observations

  • An important observation from this study involved the detection of restriction fragment length polymorphisms (RFLP) at the TCRBC locus among different horse breeds through Southern blot hybridizations. RFLP is a method used in molecular biology to compare the DNA sequences of different organisms.
  • These polymorphisms suggest that there might be a level of genetic diversity at the TCRBC locus among different horse breeds. This genetic diversity could have possible implications on the immune response variations seen in different breeds of horses.

Significance of the Research

  • The research provides significant insights into the genetic framework encoding the T-cell receptor beta chain in horses.
  • These findings can contribute to the understanding of the immune system functionality in horses and its variations across different horse breeds.
  • Further, these results can aid in developing potential strategies for effective equine health management including the detection and management of equine diseases.

Cite This Article

APA
Schrenzel MD, Watson JL, Ferrick DA. (1994). Characterization of horse (Equus caballus) T-cell receptor beta chain genes. Immunogenetics, 40(2), 135-144. https://doi.org/10.1007/BF00188177

Publication

Immunogenetics
ISSN: 0093-7711
NlmUniqueID: 0420404
Country: United States
Language: English
Volume: 40
Issue: 2
Pages: 135-144

Researcher Affiliations

Schrenzel, M D
  • Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Watson, J L
    Ferrick, D A

      MeSH Terms

      • Amino Acid Sequence
      • Animals
      • Base Sequence
      • Cloning, Molecular
      • DNA, Complementary / genetics
      • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / genetics
      • Horses / classification
      • Horses / genetics
      • Horses / immunology
      • Lymph Nodes / immunology
      • Mesentery / immunology
      • Molecular Sequence Data
      • Polymorphism, Restriction Fragment Length
      • Receptors, Antigen, T-Cell, alpha-beta / classification
      • Receptors, Antigen, T-Cell, alpha-beta / genetics
      • Sequence Analysis
      • Sequence Homology, Amino Acid
      • Sequence Homology, Nucleic Acid
      • Species Specificity

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      Citations

      This article has been cited 4 times.
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      2. Di Tommaso S, Antonacci R, Ciccarese S, Massari S. Extensive analysis of D-J-C arrangements allows the identification of different mechanisms enhancing the diversity in sheep T cell receptor beta-chain repertoire. BMC Genomics 2010 Jan 4;11:3.
        doi: 10.1186/1471-2164-11-3pubmed: 20047680google scholar: lookup
      3. Mealey RH, Littke MH, Leib SR, Davis WC, McGuire TC. Cloning and large-scale expansion of epitope-specific equine cytotoxic T lymphocytes using an anti-equine CD3 monoclonal antibody and human recombinant IL-2. Vet Immunol Immunopathol 2007 Jul 15;118(1-2):121-8.
        doi: 10.1016/j.vetimm.2007.04.001pubmed: 17498813google scholar: lookup
      4. Schrenzel MD, Ferrick DA. Horse (Equus caballus) T-cell receptor alpha, gamma, and delta chain genes: nucleotide sequences and tissue-specific gene expression. Immunogenetics 1995;42(2):112-22.
        doi: 10.1007/BF00178585pubmed: 7607702google scholar: lookup
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