Characterization of the genome of equine herpesvirus 1 subtype 2.
Abstract: The genome structure of equine herpesvirus 1 (EHV-1) subtype 2 was shown by electron microscopic studies and restriction endonuclease site mapping to comprise two covalently linked segments (L, 109 kbp; S, 35 kbp). The S segment contains a unique sequence (US) flanked by a substantial inverted repeat (TRS/IRS). Thus, the genome structure of EHV-1 subtype 2 is similar to that published previously for EHV-1 subtype 1, but the two subtypes differ in the occurrences of EcoRI and BamHI restriction sites. Hybridization studies using cloned EHV-1 DNA showed that the genome of EHV-1 subtype 2 is colinear with the genomes of EHV-1 subtype 1 and herpes simplex virus type 1. DNA sequence data for four EHV-1 subtype 2 genes, including one potentially encoding a glycoprotein, were obtained by sequencing a 4574 bp BamHI fragment containing the junction between US and TRS. The genome structure, hybridization and sequence data confirm that EHV-1 subtype 2 is of the alphaherpesvirus lineage.
Publication Date: 1988-07-01 PubMed ID: 2839595DOI: 10.1099/0022-1317-69-7-1575Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The researchers analyzed and detailed the genome structure of equine herpesvirus 1 (EHV-1) subtype 2, which is closely related to EHV-1 subtype 1 but shows differences in certain areas.
Study Methodology
- The researchers used electron microscopic studies and restriction endonuclease site mapping to investigate the structure of the EHV-1 subtype 2 genome.
- They focused on its two covalently linked segments: the larger L segment (109 kbp) and the smaller S segment (35 kbp).
- The S segment was further highlighted for its unique sequence (US) that is flanked by a substantial inverted repeat (TRS/IRS).
Comparison with EHV-1 Subtype 1
- The researchers indicated that the genome structure of EHV-1 subtype 2 is similar to that of EHV-1 subtype 1. However, they also noted significant differences in the occurrences of EcoRI and BamHI restriction sites between the two subtypes.
Hybridization Studies
- In order to determine if EHV-1 subtype 2 shares linear correspondence with other herpes viruses, they performed hybridization studies using cloned EHV-1 DNA.
- They found that the EHV-1 subtype 2 genome is colinear with the genomes of both EHV-1 subtype 1 and herpes simplex virus type 1, meaning that the sequences of their genomes align.
DNA Sequencing
- The researchers sequenced a 4574 bp BamHI fragment that contained the junction between the US and TRS in the S segment.
- They were able to obtain DNA sequence data for four EHV-1 subtype 2 genes, including one that potentially encodes a glycoprotein.
Conclusion
- The information gathered from the genome structure, hybridization, and sequence data substantiates the conclusion that the EHV-1 subtype 2 belongs to the alphaherpesvirus lineage. Alphaherpesviruses are a subfamily of herpes viruses that are generally fast-growing and can cause a range of diseases.
Cite This Article
APA
Cullinane AA, Rixon FJ, Davison AJ.
(1988).
Characterization of the genome of equine herpesvirus 1 subtype 2.
J Gen Virol, 69 ( Pt 7), 1575-1590.
https://doi.org/10.1099/0022-1317-69-7-1575 Publication
Researcher Affiliations
- Medical Research Council Virology Unit, Institute of Virology, U.K.
MeSH Terms
- Amino Acid Sequence
- Base Sequence
- DNA, Viral / genetics
- DNA, Viral / ultrastructure
- Genes, Viral
- Herpesviridae / genetics
- Herpesvirus 1, Equid / classification
- Herpesvirus 1, Equid / genetics
- Molecular Sequence Data
- Sequence Homology, Nucleic Acid
Citations
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