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Clenbuterol affects the expression of messenger RNA for interleukin 10 in peripheral leukocytes from horses challenged intrabronchially with lipopolysaccharides.
Abstract: On four occasions, four horses with heaves and four horses with small airway inflammatory diseases inhaled 0.9% saline based aerosol mixtures with or without lipopolysaccharides (LPS). Prior to the first saline and LPS inhalation, horses were untreated, while three and a half days prior to the third and forth inhalation horses had received 0.8 microg/kg clenbuterol intravenously twice daily. The messenger RNA (mRNA) expression of tumour necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, IL-4, IL-6, IL-8, IL-10 and interferon- gamma (IFN- gamma) was investigated by RT-PCR, all of which were expressed in the white blood cells of samples collected. Inhalation of LPS only changed the cytokine expression profile of IL-10, IL-4 and TNF-alpha mRNA which were higher after challenge with LPS. However in those horses that were treated with clenbuterol the LPS-induced IL-10 mRNA expression was shown to be suppressed. Further changes in IL-4 and TNF-alpha were not significant. Thus the results of this study indicated that clenbuterol can modulate the expression of IL-10 mRNA in peripheral white blood cells in those horses with small airway diseases that have been exposed to LPS.
Publication Date: 2006-12-02 PubMed ID: 17139543DOI: 10.1007/s11259-006-3383-4Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Controlled Clinical Trial
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study explores how clenbuterol, a medication used to treat breathing disorders, affects the expression of interleukin 10 (IL-10) in horses with small airway diseases when exposed to lipopolysaccharides (LPS), a substance known to stimulate immune responses. It found that clenbuterol suppresses IL-10 expression in these circumstances.
Explanation of Research
The research focuses on the effects of clenbuterol on the expression of various cytokines in horses with respiratory conditions known as heaves and small airway inflammatory diseases. It specifically investigates how this drug interacts with lipopolysaccharides (LPS) to influence the expression of interleukin 10 (IL-10) in the horses’ peripheral leukocytes or white blood cells. The other cytokines studied were tumour necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-8 (IL-8), and interferon-gamma (IFN-gamma).
- The experiment was conducted on four occasions with four horses diagnosed with heaves and four horses diagnosed with small airway inflammatory diseases.
- The horses inhaled saline-based aerosol mixtures that either contained or did not contain LPS. The LPS was introduced to stimulate an immune response.
- Before the first saline and LPS inhalation, the horses were not given any treatment. However, before the third and fourth inhalation, the horses were given clenbuterol intravenously.
- The researchers then used Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), a molecular biology technique, to analyze the expression levels of the different cytokines in the white blood cells of the horses.
Findings of the Study
The research findings provide valuable insight into the effects of clenbuterol on cytokine expression when the immune system of horses is challenged with LPS.
- The inhalation of LPS only changed the cytokine expression profile for IL-10, IL-4, and TNF-alpha, with the expression of these cytokines being higher after LPS challenge.
- When the horses were treated with clenbuterol, the LPS-induced IL-10 mRNA expression was found to be suppressed.
- There were no significant changes observed in the expression of IL-4 and TNF-alpha after treatment with clenbuterol.
- The study concludes that clenbuterol can modulate, specifically suppress, the expression of IL-10 in horses with small airway diseases exposed to LPS.
This research is important for veterinary medicine as it offers potential approaches to treat respiratory diseases in horses by mitigating the effects of LPS exposure. It also helps in understanding the role of clenbuterol and how it influences the immune response.
Cite This Article
APA
van den Hoven R, Duvigneau JC, Hartl RT, Gemeiner M.
(2006).
Clenbuterol affects the expression of messenger RNA for interleukin 10 in peripheral leukocytes from horses challenged intrabronchially with lipopolysaccharides.
Vet Res Commun, 30(8), 921-928.
https://doi.org/10.1007/s11259-006-3383-4 Publication
Researcher Affiliations
- Department of Companion Animals and Horses, Clinic for Internal Medicine and Infectious Diseases, Institute of Medical Chemistry, Veterinary University of Vienna, Veterinärplatz 1, A-1210 Vienna, Austria. Rene.vandenHoven@vu-wien.ac.at.
MeSH Terms
- Animals
- Bronchodilator Agents / administration & dosage
- Bronchodilator Agents / pharmacology
- Bronchodilator Agents / therapeutic use
- Clenbuterol / pharmacology
- Gene Expression Regulation / drug effects
- Horse Diseases / drug therapy
- Horse Diseases / metabolism
- Horses
- Interleukin-10 / genetics
- Leukocytes / drug effects
- Leukocytes / metabolism
- Lipopolysaccharides / administration & dosage
- Lipopolysaccharides / pharmacology
- Lung Diseases, Obstructive / metabolism
- Lung Diseases, Obstructive / veterinary
- Male
- RNA, Messenger / genetics
- RNA, Messenger / metabolism
References
This article includes 12 references
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Citations
This article has been cited 4 times.- Nguyen TNB, Ely BA, Pick D, Patel M, Xie H, Kim-Schulze S, Gabbay V. Clenbuterol attenuates immune reaction to lipopolysaccharide and its relationship to anhedonia in adolescents. Brain Behav Immun 2022 Nov;106:89-99.
- Witkowska-Piłaszewicz O, Pingwara R, Szczepaniak J, Winnicka A. The Effect of the Clenbuterol-β2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen Species Production in Race Horses In Vitro. Cells 2021 Apr 17;10(4).
- Riihimäki M, Raine A, Elfman L, Pringle J. Markers of respiratory inflammation in horses in relation to seasonal changes in air quality in a conventional racing stable. Can J Vet Res 2008 Oct;72(5):432-9.
- Riihimäki M, Raine A, Pourazar J, Sandström T, Art T, Lekeux P, Couëtil L, Pringle J. Epithelial expression of mRNA and protein for IL-6, IL-10 and TNF-alpha in endobronchial biopsies in horses with recurrent airway obstruction. BMC Vet Res 2008 Feb 23;4:8.
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