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Home / Equine Research Bank / Infect Immun / Cloning and sequence analysis of a protective M-like protein...
Infection and immunity1995; 63(4); 1440-1445; doi: 10.1128/iai.63.4.1440-1445.1995

Cloning and sequence analysis of a protective M-like protein gene from Streptococcus equi subsp. zooepidemicus.

Abstract: Streptococcus equi subsp. zooepidemicus, a Lancefield group C streptococcus, is a frequently isolated opportunist pathogen from a variety of animal hosts, including the horse. Previous studies have indicated that equine strains carry antigens with characteristics of the antiphagocytic M proteins on the Lancefield groups A and G streptococci. We have cloned a protective M-like protein gene (SzPW60) of an equine strain of S. equi subsp. zooepidemicus W60 and determined its sequence. This gene encodes a protein with a molecular weight of 40,123 which protects mice against subsp. zooepidemicus but not subsp. equi, stimulates antibodies which opsonize subsp. zooepidemicus but not equi, and reacts with antiserum to the protein of the parent strain. The predicted amino acid structure shows significant homology with the carboxy termini of groups A and G M proteins but no other homology. The M-like protein, although showing an extensive region of alpha helix, lacks the A, B, and C repeats found in group A M proteins and has a shorter signal sequence. A proline-rich region upstream from the LPSTGE motif contains 20 repeats of the tetrapeptide PEPK. The presence of this repeat region may account for the slow migration of the M-like protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
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Publication Date: 1995-04-01 PubMed ID: 7890407PubMed Central: PMC173172DOI: 10.1128/iai.63.4.1440-1445.1995Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article highlights the isolation and sequence analysis of a protective M-like protein gene from a uniform subspecies of Streptococcus equi, specifically zooepidemicus, which oftentimes poses as an opportunistic pathogen in numerous animal hosts such as horses. The gene, labeled as SzPW60, not only displays a significant degree of similarity with the amino acid structure of groups A & G M proteins but also stimulates antibodies for protection against Streptococcus equi subspecies zooepidemicus.

Objective of the Research

  • The major objective of the research study was to clone and analyse the sequence of a protective M-like protein gene (SzPW60) from Streptococcus equi subsp. zooepidemicus, a pathogen commonly found in various animal hosts.

Research Methodology and Findings

  • The researchers identified and cloned the SzPW60 gene from an equine strain of the S. equi subsp. zooepidemicus.
  • The gene was found to stimulate antibodies that specifically counteracted the zooepidemicus subspecies and not any other strain. It also reacted with the antiserum acquired from the parent strain.
  • The researchers also conducted a comprehensive sequence analysis of the gene and discovered its amino acid structure. It revealed a significant similarity with the carboxy termini of Group A and G M proteins, despite having a distinct identity.
  • The gene-encoded protein, having a molecular weight of 40,123, demonstrated substantial protective capabilities against the zooepidemicus subspecies.

Interpretation of the Results

  • The researchers inferred that the SzPW60 gene contains crucial properties that may contribute to the development of targeted prevention measures against S. equi subsp. zooepidemicus.
  • The protein’s extensive region of alpha helix lacked the repeat sequences found in group A M proteins and had a shorter signal sequence, which could account for its unique characteristics and function.
  • The presence of a proline-rich region in the gene could result in its slow migration during sodium dodecyl sulfate-polyacrylamide gel electrophoresis, a method commonly used to isolate proteins.

Cite This Article

APA
Timoney JF, Walker J, Zhou M, Ding J. (1995). Cloning and sequence analysis of a protective M-like protein gene from Streptococcus equi subsp. zooepidemicus. Infect Immun, 63(4), 1440-1445. https://doi.org/10.1128/iai.63.4.1440-1445.1995

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 63
Issue: 4
Pages: 1440-1445

Researcher Affiliations

Timoney, J F
  • Gluck Equine Research Center, University of Kentucky, Lexington 40546-0099.
Walker, J
    Zhou, M
      Ding, J

        MeSH Terms

        • Amino Acid Sequence
        • Antigens, Bacterial / genetics
        • Bacterial Proteins / genetics
        • Base Sequence
        • Cloning, Molecular
        • DNA, Bacterial / genetics
        • Genes, Bacterial
        • Molecular Sequence Data
        • Molecular Weight
        • Opsonin Proteins
        • Protein Structure, Secondary
        • Restriction Mapping
        • Sequence Alignment
        • Sequence Homology, Amino Acid
        • Streptococcus equi / immunology

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        Citations

        This article has been cited 19 times.
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