Comparative analysis of phagocytosis of fungal cells by insect hemocytes versus horse neutrophils.
Abstract: In this study, the phagocytic ability of Spodoptera exigua hemocytes was compared to horse neutrophils. In vitro assays showed that the insect granulocytes and horse neutrophils actively phagocytose FITC-labeled Paecilomyces farinosus blastospores opsonized with S. exigua hemolymph lectin or horse serum, respectively. Killing of fungal cells by the neutrophils and hemocytes was analyzed under in vitro conditions. Neutrophils reduced the growth of P. farinosus up to 65% whereas no fungicidal activity was observed with hemocyte monolayers. The production of oxygen metabolites by both phagocytic cells incubated with various elicitors (fungal cells, bacteria, phorbol myristate acetate) was examined using luminol-enhanced chemiluminescence. Phagocytosis of opsonized microbes by horse neutrophils resulted in marked increase of chemiluminescence activity whereas no chemiluminescence was detected in similarly challenged phagocytic insect hemocytes. Electron microscopy was used to examine phagocytic events and confirmed that insect phagocytes were unable to kill tested microbes.
Publication Date: 1994-11-01 PubMed ID: 7768313DOI: 10.1016/s0145-305x(06)80001-7Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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This research compares how insect immune cells (hemocytes) and horse cells (neutrophils) consume and kill foreign harmful cells (phagocytosis). The results showed that while both cell types consume such cells, the horse cells were significantly more successful at killing them.
Objective of the Study
- The research aimed to conduct a comparative analysis of the phagocytic abilities (how cells ingest and destroy foreign cells) of hemocytes from the insect Spodoptera exigua and neutrophils from horses.
Methodology and Results
- The study made use of in vitro assays, providing an environment outside of a living organism to observe the phagocytic behaviour of hemocytes and neutrophils.
- Both cell types were exposed to FITC-labeled Paecilomyces farinosus blastospores that were treated with S. exigua hemolymph lectin or horse serum, to promote phagocytosis.
- It was observed that the horse neutrophils showed a significant reduction in the growth of P. farinosus up to 65%, indicating high phagocytic activity. However, there was no observable fungicidal activity with the insect hemocyte cultures.
Chemiluminescence and Electron Microscopy
- The production of reactive oxygen species by the cells during phagocytosis was examined using luminol-enhanced chemiluminescence. Notably, horse neutrophils displayed a marked increase in chemiluminescence activity, indicating the generation of more oxidative products, whereas no such activity was detected in the insect hemocytes.
- Further, the phagocytosis process was observed using electron microscopy, which further confirmed the lack of killing ability of the insect phagocytes against the tested microbes.
Conclusions
- The study concludes that while both insect hemocytes and horse neutrophils possess phagocytic capabilities, there is a significant difference in their abilities to kill off the foreign cells. The neutrophils from horse were notably more effective in their fungicidal activity compared to the hemocytes from Spodoptera exigua.
Cite This Article
APA
Mazet I, Pendland J, Boucias D.
(1994).
Comparative analysis of phagocytosis of fungal cells by insect hemocytes versus horse neutrophils.
Dev Comp Immunol, 18(6), 455-466.
https://doi.org/10.1016/s0145-305x(06)80001-7 Publication
Researcher Affiliations
- Department of Entomology, University of Florida, Gainesville 32611, USA.
MeSH Terms
- Animals
- Hemocytes / immunology
- Hemocytes / ultrastructure
- Horses / immunology
- Luminescent Measurements
- Macrophage Activation
- Microscopy, Fluorescence
- Neutrophils / immunology
- Neutrophils / ultrastructure
- Paecilomyces / immunology
- Phagocytosis / immunology
- Reactive Oxygen Species / chemistry
- Spodoptera / immunology
- Tetradecanoylphorbol Acetate / pharmacology
Citations
This article has been cited 2 times.- Barthel A, Kopka I, Vogel H, Zipfel P, Heckel DG, Groot AT. Immune defence strategies of generalist and specialist insect herbivores. Proc Biol Sci 2014 Aug 7;281(1788):20140897.
- Hyrsl P, Cíz M, Kubala L, Lojek A. Silkworm (Bombyx mori) hemocytes do not produce reactive oxygen metabolites as a part of defense mechanisms. Folia Microbiol (Praha) 2004;49(3):315-9.
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