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Biorheology1996; 33(4-5); 365-377; doi: 10.1016/0006-355x(96)00028-5

Comparison and simulation of different levels of erythrocyte aggregation with pig, horse, sheep, calf, and normal human blood.

Abstract: Erythrocyte aggregation levels in pig, horse, sheep, and calf blood samples were investigated and compared to that of normal human blood. The aggregation kinetics and adhesive forces between red cells, and an index of structure of the aggregates were determined with an erythroaggregameter (Regulest, France) at constant hematocrit (0.40 l/l) and temperature (37 degrees C). The adhesive forces and the index of structure in pig blood were close to those of normal human blood. The results for horse blood showed a very high level of aggregation kinetics and adhesive forces between red cells. For sheep and calf blood, little erythrocyte aggregation was found. To simulate different levels of red cell hyperaggregation in humans, a volume of horse plasma was replaced by isotonic NaCl in different proportions (5 to 40% V/V). The kinetics of rouleaux formation and especially the adhesive forces between erythrocytes were systematically decreased, while the index of structure was raised with increasing concentrations of isotonic NaCl. By replacing the porcine plasma with isotonic NaCl, normal and hypoaggregating levels of human red cells were simulated. The aggregation kinetics and the adhesive forces were reduced and the index of structure was raised when the concentration of isotonic NaCl was increased. In summary, large differences in the aggregation parameters were found between mammals. This study also showed that different human erythrocyte aggregation levels can be simulated by diluting the concentration of plasma proteins in equine and porcine bloods.
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Publication Date: 1996-07-01 PubMed ID: 8977661DOI: 10.1016/0006-355x(96)00028-5Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores how blood cell aggregation levels in pig, horse, sheep, and calf blood compare to those found in human blood. The results show that pig blood is most similar to human blood, while horse blood exhibits a high level of aggregation and adhesive forces. Sheep and calf blood show little aggregation. Experiments were also conducted to simulate different levels of human blood cell aggregation by adjusting the concentration of plasma proteins in equine and porcine blood.

Methodology of the Study

  • The researchers conducted a comparative study using blood samples taken from pigs, horses, sheep, calves, and humans.
  • To measure and compare erythrocyte aggregation levels, they utilized an erythroaggregameter and conducted experiments under constant condition— hematocrit at 0.40 l/l and temperature at 37 degrees C.
  • They assessed aggregation kinetics, adhesive forces between red cells, and the structure of the aggregates.

Results from Comparative Study

  • The study found that the adhesive forces and structure index in pig blood were close to those of normal human blood.
  • Horse blood, on the other hand, displayed a high level of aggregation and adhesive forces.
  • In contrast, sheep and calf blood exhibited little erythrocyte aggregation.

Simulation Experiments

  • Building on these findings, the researchers then attempted to simulate different levels of human red cell hyperaggregation.
  • They replaced varying amounts of horse plasma with isotonic NaCl (ranging from 5 to 40% volume/volume) and recorded the changes in rouleaux formation kinetics, adhesive forces, and the structure index.
  • The researchers also did a similar study with porcine blood to reproduce normal and hypoaggregating levels of human red cells.
  • In both cases, as the concentration of isotonic NaCl increased, the aggregation kinetics and adhesive forces were reduced, while the structure index rose.

Key Takeaways

  • The research found significant differences in erythrocyte aggregation parameters across various mammals. Among the tested animals, pig blood was most similar to human blood in terms of aggregation characteristics, while horse blood demonstrated significant aggregation and adhesive forces.
  • In their simulated conditions, the researchers demonstrated that different human erythrocyte aggregation levels could be replicated by altering plasma protein concentration in equine and porcine blood.”

Cite This Article

APA
Weng X, Cloutier G, Pibarot P, Durand LG. (1996). Comparison and simulation of different levels of erythrocyte aggregation with pig, horse, sheep, calf, and normal human blood. Biorheology, 33(4-5), 365-377. https://doi.org/10.1016/0006-355x(96)00028-5

Publication

Biorheology
ISSN: 0006-355X
NlmUniqueID: 0372526
Country: Netherlands
Language: English
Volume: 33
Issue: 4-5
Pages: 365-377

Researcher Affiliations

Weng, X
  • Laboratory of Biomedical Engineering, Clinical Research Institute of Montreal, Québec, Canada.
Cloutier, G
    Pibarot, P
      Durand, L G

        MeSH Terms

        • Animals
        • Cattle / blood
        • Erythrocyte Aggregation
        • Erythrocytes / cytology
        • Hemagglutination Tests / instrumentation
        • Horses / blood
        • Humans
        • Kinetics
        • Lasers
        • Models, Biological
        • Sheep / blood
        • Swine / blood

        Citations

        This article has been cited 12 times.
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