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Home / Equine Research Bank / BMC Vet Res / Comparison between adult and foetal adnexa derived equine po...
BMC veterinary research2019; 15(1); 277; doi: 10.1186/s12917-019-2023-5

Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells.

Abstract: Little is known about the differences among adult and foetal equine mesenchymal stem cells (MSCs), and no data exist about their comparative ultrastructural morphology. The aim of this study was to describe and compare characteristics, immune properties, and ultrastructural morphology of equine adult (bone marrow: BM, and adipose tissue: AT) and foetal adnexa derived (umbilical cord blood: UCB, and Wharton's jelly: WJ) MSCs. Results: No differences were observed in proliferation during the first 3 passages. While migration ability was similar among cells, foetal MSCs showed a higher adhesion ability, forming smaller spheroids after hanging drop culture (P < 0.05). All MSCs differentiated toward adipogenic, chondrogenic and osteogenic lineages, only tenogenic differentiation was less evident for WJ-MSCs. Data obtained by PCR confirmed MHC1 expression and lack of MHC2 expression in all four cell types. Foetal adnexa MSCs were positive for genes specific for anti-inflammatory and angiogenic factors (IL6, IL8, ILβ1) and WJ-MSCs were the only positive for OCT4 pluripotency gene. At immunofluorescence all cells expressed typical mesenchymal markers (α-SMA, N-cadherin), except for BM-MSCs, which did not express N-cadherin. By transmission electron microscopy, it was observed that WJ-MSCs had a higher (P < 0.05) number of microvesicles compared to adult MSCs, and UCB-MSCs showed more microvesicles than BM-MSCs (P < 0.05). AT-MSCs had a lower number of mitochondria than WJ-MSCs (P < 0.05), and mitochondrial area was higher for WJ-MSCs compared to UCB and AT-MSCs (P < 0.05). Conclusions: Results demonstrate that MSCs from adult and foetal tissues have different characteristics, and foetal MSCs, particularly WJ derived ones, seem to have some charactestics that warrant further investigation into potential advantages for clinical application.
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Publication Date: 2019-08-02 PubMed ID: 31375144PubMed Central: PMC6679462DOI: 10.1186/s12917-019-2023-5Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research sought to explore and compare the characteristics, immune properties, and ultrastructural morphology of adult and foetal equine mesenchymal stem cells (MSCs). The outcomes demonstrated that both categories possess distinct attributes, particularly in the foetal MSCs derived from Wharton’s jelly, which might have potential advantages for clinical application.

Objective of the Research

  • The primary objective of this study was to investigate and contrast the characteristics, immune properties, and ultrastructural morphology of both adult and foetal mesenchymal stem cells (MSCs) in horses.
  • The researchers sought to fill a knowledge gap as prior to this, there was little information available comparing adult and foetal equine MSCs, particularly with regards to their ultrastructural morphology.

Methodology and Findings

The team observed the following:

  • There was no noticeable variation in proliferation during the first three cell passages.
  • The migration capacity was consistent across the cells, but the foetal MSCs displayed a higher adherence ability.
  • All the MSCs showed differentiation potential towards adipogenic, chondrogenic and osteogenic lineages. However, Wharton’s jelly-MSCs (WJ-MSCs) showed less differentiation potential towards tenogenic lineages.
  • Immune properties of the cells were also examined; all four cell sources (adult bone marrow and adipose tissue, and foetal umbilical cord blood and Wharton’s jelly) expressed MHC1 but not MHC2.
  • Additionally, foetal MSCs were positive for genes specific for anti-inflammatory and angiogenic factors such as IL6, IL8, ILβ1. WJ-MSCs were unique in expressing the pluripotency gene OCT4.
  • Regarding ultrastructure, WJ-MSCs had a higher number of microvesicles compared to adult MSCs, and UCB-MSCs showed more microvesicles than BM-MSCs. There were fewer mitochondria in AT-MSCs than WJ-MSCs, and the mitochondrial area was greater for WJ-MSCs compared to UCB and AT-MSCs.

Conclusion and Implications

  • The results demonstrated that MSCs from adult and foetal tissues have different characteristics. Particularly, foetal MSCs, especially those derived from Wharton’s jelly, seemed to display some qualities that may present potential advantages for clinical application.
  • The implications of this research potentially impact not only equine medicine but may also contribute to the broader field of stem cell research and its application in other animals or humans. It opens up avenues for future comparison studies of stem cells derived from different tissue sources.

Cite This Article

APA
Merlo B, Teti G, Lanci A, Burk J, Mazzotti E, Falconi M, Iacono E. (2019). Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells. BMC Vet Res, 15(1), 277. https://doi.org/10.1186/s12917-019-2023-5

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 15
Issue: 1
Pages: 277
PII: 277

Researcher Affiliations

Merlo, B
  • Department of Veterinary Medical Sciences, University of Bologna, via Tolara di Sopra 50, 40064, Ozzano Emilia, BO, Italy.
  • Health Science and Technologies Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Bologna, Italy.
Teti, G
  • Department for Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
Lanci, A
  • Department of Veterinary Medical Sciences, University of Bologna, via Tolara di Sopra 50, 40064, Ozzano Emilia, BO, Italy.
Burk, J
  • Saxon Incubator for Clinical Translation, University of Leipzig, Leipzig, Germany.
  • Equine Clinic (Surgery), Justus Liebig University Giessen, Giessen, Germany.
Mazzotti, E
  • Department of Comparative Biomedical Sciences, University of Teramo, Teramo, Italy.
Falconi, M
  • Department for Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
Iacono, E
  • Department of Veterinary Medical Sciences, University of Bologna, via Tolara di Sopra 50, 40064, Ozzano Emilia, BO, Italy. eleonora.iacono2@unibo.it.
  • Health Science and Technologies Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Bologna, Italy. eleonora.iacono2@unibo.it.

MeSH Terms

  • Adult Stem Cells / physiology
  • Animals
  • Cell Differentiation
  • Cell Migration Assays
  • Cell Proliferation
  • Cellular Senescence
  • Fetal Blood / cytology
  • Horses
  • Mesenchymal Stem Cells
  • Wharton Jelly / cytology

Conflict of Interest Statement

The authors declare that they have no competing interests.

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