Comparison of culture and insemination techniques for equine oocyte transfer.
Abstract: This study was designed to test 3 approaches for insemination and transfer of oocytes to recipient mares. Oocytes were recovered transvaginally from naturally cycling donor mares 24 to 26 h after an intravenous injection of 2500 IU of hCG when follicles reached 35 mm in diameter. Multiple oocytes (1 to 4) were transferred surgically into the oviducts of 4 or 5 recipient mares per group. Three groups of transfers were compared: 1) transfer of oocytes cultured in vitro for 12 to 14 h postcollection with insemination of the recipient 2 h postsurgery; 2) transfer of oocytes into the oviduct within 1 h of collection, with completion of oocyte maturation occurring within the oviduct, and insemination of the recipient 14 to 16 h postsurgery; and 3) transfer of spermatozoa and oocytes (cultured 12 to 14 h in vitro) into the oviduct. Numbers of embryos detected by Day 16 of gestation were not different (P>0. 1) for groups 1, 2, and 3 (57%, 43% and 27%). Therefore, equine oocytes successfully completed the final stages of maturation within the oviduct, and sperm deposited within the oviduct were capable of fertilizing oocytes.
Publication Date: 2000-11-30 PubMed ID: 11097049DOI: 10.1016/S0093-691X(00)00406-4Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research paper investigates and compares different techniques for equine oocyte transfer and insemination, with the resultant embryos examined up to day 16 of gestation.
Objective of the Study
- The study aimed to test three methods of transferring and inseminating oocytes (cells from which an egg or ovum develops by meiosis) into recipient mares (female horses).
Procedure of the Experiment
- Oocytes were harvested from naturally cycling donor mares after they were given an hCG injection as soon as their follicles reached 35 mm in diameter.
- The oocytes were then transferred into the oviducts of the recipient mares. Each recipient mare received between one and four oocytes. The transfer processes took place in three groups each with a different approach.
Different Approaches for Transfer and Insemination
- Group 1: The oocytes were cultured in vitro (outside the body in a lab) for around 12 to 14 hours after being collected. The recipient was then inseminated two hours after the surgery.
- Group 2: The oocytes were transferred into the recipient’s oviduct within an hour after collection and the maturation of the oocyte took place inside the oviduct. The insemination took place between 14 to 16 hours after the surgery.
- Group 3: Both the sperm and oocytes (which were cultured in vitro for 12-14 hours) were transferred into the recipient’s oviduct.
Results of the Study
- By the 16th day of gestation, the number of embryos detected were not significantly different among the three groups. The success rates were 57%, 43%, and 27% for Group 1, Group 2, and Group 3 respectively.
- The results show that oocytes can successfully mature inside the oviduct, and insemination with sperm deposited in the oviduct is capable of fertilizing these oocytes.
Cite This Article
APA
Carnevale EM, Maclellan LJ, Coutinho da Silva MA, Scott TJ, Squires EL.
(2000).
Comparison of culture and insemination techniques for equine oocyte transfer.
Theriogenology, 54(6), 981-987.
https://doi.org/10.1016/S0093-691X(00)00406-4 Publication
Researcher Affiliations
- Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins 80523, USA.
MeSH Terms
- Animals
- Chorionic Gonadotropin / administration & dosage
- Female
- Fertilization in Vitro / veterinary
- Gamete Intrafallopian Transfer / methods
- Gamete Intrafallopian Transfer / veterinary
- Horses / physiology
- Inhalation
- Insemination, Artificial / veterinary
- Laparotomy / veterinary
- Male
- Oocytes / physiology
- Ovarian Follicle / physiology
- Pregnancy
Citations
This article has been cited 3 times.- Maclellan LJ, Albertini DF, Stokes JE, Carnevale EM. Use of confocal microscopy and intracytoplasmic sperm injection (ICSI) to assess viability of equine oocytes from young and old mares after vitrification. J Assist Reprod Genet 2023 Nov;40(11):2565-2576.
- Lawson EF, Grupen CG, Baker MA, Aitken RJ, Swegen A, Pollard CL, Gibb Z. Conception and early pregnancy in the mare: lipidomics the unexplored frontier. Reprod Fertil 2022 Jan 1;3(1):R1-R18.
- Ruggeri E, DeLuca KF, Galli C, Lazzari G, DeLuca JG, Stokes JE, Carnevale EM. Use of Confocal Microscopy to Evaluate Equine Zygote Development After Sperm Injection of Oocytes Matured In Vivo or In Vitro. Microsc Microanal 2017 Dec;23(6):1197-1206.
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