FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Am J Vet Res / Comparison of equine tendon- and bone marrow-derived cells c...
American journal of veterinary research2011; 73(1); 153-161; doi: 10.2460/ajvr.73.1.153

Comparison of equine tendon- and bone marrow-derived cells cultured on tendon matrix with or without insulin-like growth factor-I supplementation.

Abstract: To compare in vitro expansion, explant colonization, and matrix synthesis of equine tendon- and bone marrow-derived cells in response to insulin-like growth factor-I (IGF-I) supplementation. Methods: Cells isolated from 7 young adult horses. Methods: Tendon- and bone marrow-derived progenitor cells were isolated, evaluated for yield, and cultured on autogenous cell-free tendon matrix for 7 days. Samples were analyzed for cell viability and expression of collagen type I, collagen type III, and cartilage oligomeric matrix protein mRNAs. Collagen and glycosaminoglycan syntheses were quantified over a 24-hour period. Results: Tendon- and bone marrow-derived cells required 17 to 19 days of monolayer culture to reach 2 passages. Mean ± SE number of monolayer cells isolated was higher for tendon-derived cells (7.9 ± 0.9 × 10(6)) than for bone marrow-derived cells (1.2 ± 0.1 × 10(6)). Cell numbers after culture for 7 days on acellular tendon matrix were 1.6- to 2.8-fold higher for tendon-derived cells than for bone marrow-derived cells and 0.8- to 1.7-fold higher for IGF-I supplementation than for untreated cells. New collagen and glycosaminoglycan syntheses were significantly greater in tendon-derived cell groups and in IGF-I-supplemented groups. The mRNA concentrations of collagen type I, collagen type III, and cartilage oligomeric matrix protein were not significantly different between tendon- and bone marrow-derived groups. Conclusions: In vitro results of this study suggested that tendon-derived cells supplemented with IGF-I may offer a useful resource for cell-based strategies in tendon healing.
Get Full Text
Publication Date: 2011-12-30 PubMed ID: 22204302DOI: 10.2460/ajvr.73.1.153Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Evaluation Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examined and compared how cells derived from horse tendons and bone marrow respond to insulin-like growth factor-I (IGF-I) supplementation. The study found that both cells types, when supplemented with IGF-I, could be potential resources in tendon healing procedures.

Study Methodology

  • The study involved cells taken from the bone marrow and tendons of 7 young adult horses.
  • Both types of cells were cultured on tendon matrices for a week without any cells, known as autogenous cell-free tendon matrix.
  • The cells were then examined for their viability (ability to live and grow) and their production of collagen type I, collagen type III, and cartilage oligomeric matrix protein mRNAs, which are essential components of the extracellular matrix in tendons.
  • The creation of collagen and glycosaminoglycans, essential components in the tendon matrix, was measured over 24 hours.

Results

  • Both tendon-derived and bone marrow-derived cells required 17 to 19 days in a monolayer culture to accomplish 2 cell divisions (passages).
  • The tendon-derived cells yielded a significantly higher number of cells compared to bone marrow-derived cells.
  • After a week of being cultured on the tendon matrix, the cell count for tendon-derived cells was 1.6 to 2.8 times higher than for bone marrow-derived cells and was 0.8 to 1.7 times higher in the IGF-I supplemented cells compared to the untreated cells.
  • New collagen and glycosaminoglycan syntheses were much higher in tendon-derived cell groups and in IGF-I-supplemented groups.
  • No significant difference was found in the mRNA concentrations of collagen type I, collagen type III, and cartilage oligomeric matrix protein between tendon- and bone marrow-derived cells.

Conclusions

  • The in vitro results suggest that tendon-derived cells, when supplemented with IGF-I, could be a promising resource for cell-based therapeutic strategies in tendon healing.
  • While bone marrow-derived cells also showed potential, the tendon-derived cells had a clear advantage in terms of cell yield and the increased production of essential tendon matrix compounds when supplemented with IGF-I.

Cite This Article

APA
Durgam SS, Stewart AA, Pondenis HC, Gutierrez-Nibeyro SM, Evans RB, Stewart MC. (2011). Comparison of equine tendon- and bone marrow-derived cells cultured on tendon matrix with or without insulin-like growth factor-I supplementation. Am J Vet Res, 73(1), 153-161. https://doi.org/10.2460/ajvr.73.1.153

Publication

American journal of veterinary research
ISSN: 1943-5681
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 73
Issue: 1
Pages: 153-161

Researcher Affiliations

Durgam, Sushmitha S
  • Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.
Stewart, Allison A
    Pondenis, Holly C
      Gutierrez-Nibeyro, Santiago M
        Evans, Richard B
          Stewart, Matthew C

            MeSH Terms

            • Animals
            • Blotting, Northern / veterinary
            • Bone Marrow Cells / cytology
            • Bone Marrow Cells / drug effects
            • Bone Marrow Cells / metabolism
            • Bone Marrow Cells / physiology
            • Cell Culture Techniques / methods
            • Cell Culture Techniques / veterinary
            • Collagen Type I / biosynthesis
            • Collagen Type III / biosynthesis
            • Extracellular Matrix / drug effects
            • Extracellular Matrix / metabolism
            • Extracellular Matrix Proteins / biosynthesis
            • Gene Expression Regulation
            • Glycoproteins / biosynthesis
            • Glycosaminoglycans / biosynthesis
            • Horses / metabolism
            • Insulin-Like Growth Factor I / pharmacology
            • Matrilin Proteins
            • RNA, Messenger / metabolism
            • Real-Time Polymerase Chain Reaction / veterinary
            • Tendons / cytology
            • Tendons / drug effects
            • Tendons / growth & development
            • Tendons / metabolism

            Citations

            This article has been cited 16 times.
            1. Fu S, Lan Y, Wang G, Bao D, Qin B, Zheng Q, Liu H, Wong VKW. External stimulation: A potential therapeutic strategy for tendon-bone healing.. Front Bioeng Biotechnol 2023;11:1150290.
              doi: 10.3389/fbioe.2023.1150290pubmed: 37064229google scholar: lookup
            2. Tsiapalis D, Kearns S, Kelly JL, Zeugolis DI. Growth factor and macromolecular crowding supplementation in human tenocyte culture.. Biomater Biosyst 2021 Mar;1:100009.
              doi: 10.1016/j.bbiosy.2021.100009pubmed: 36825160google scholar: lookup
            3. Miescher I, Rieber J, Calcagni M, Buschmann J. In Vitro and In Vivo Effects of IGF-1 Delivery Strategies on Tendon Healing: A Review.. Int J Mol Sci 2023 Jan 25;24(3).
              doi: 10.3390/ijms24032370pubmed: 36768692google scholar: lookup
            4. Rocha J, Araújo JC, Fangueiro R, Ferreira DP. Wetspun Polymeric Fibrous Systems as Potential Scaffolds for Tendon and Ligament Repair, Healing and Regeneration.. Pharmaceutics 2022 Nov 19;14(11).
              doi: 10.3390/pharmaceutics14112526pubmed: 36432717google scholar: lookup
            5. Park J, Jo S, Lee MK, Kim TH, Sung IH, Lee JK. Comparison of ligamentization potential between anterior cruciate ligament-derived cells and adipose-derived mesenchymal stem cells reseeded to acellularized tendon allograft.. Bone Joint Res 2022 Nov;11(11):777-786.
              doi: 10.1302/2046-3758.1111.BJR-2021-0548.R2pubmed: 36342052google scholar: lookup
            6. Roberts JH, Halper J. Growth Factor Roles in Soft Tissue Physiology and Pathophysiology.. Adv Exp Med Biol 2021;1348:139-159.
              doi: 10.1007/978-3-030-80614-9_6pubmed: 34807418google scholar: lookup
            7. Sullivan SN, Altmann NN, Brokken MT, Durgam SS. In vitro Effects of Methylprednisolone Acetate on Equine Deep Digital Flexor Tendon-Derived Cells.. Front Vet Sci 2020;7:486.
              doi: 10.3389/fvets.2020.00486pubmed: 32851046google scholar: lookup
            8. Durgam SS, Altmann NN, Coughlin HE, Rollins A, Hostnik LD. Insulin Enhances the In Vitro Osteogenic Capacity of Flexor Tendon-Derived Progenitor Cells.. Stem Cells Int 2019;2019:1602751.
              doi: 10.1155/2019/1602751pubmed: 31949435google scholar: lookup
            9. Abdelrazik H, Giordano E, Barbanti Brodano G, Griffoni C, De Falco E, Pelagalli A. Substantial Overview on Mesenchymal Stem Cell Biological and Physical Properties as an Opportunity in Translational Medicine.. Int J Mol Sci 2019 Oct 29;20(21).
              doi: 10.3390/ijms20215386pubmed: 31671788google scholar: lookup
            10. Shojaee A, Parham A. Strategies of tenogenic differentiation of equine stem cells for tendon repair: current status and challenges.. Stem Cell Res Ther 2019 Jun 18;10(1):181.
              doi: 10.1186/s13287-019-1291-0pubmed: 31215490google scholar: lookup
            11. Ganji E, Killian ML. Tendon healing in the context of complex fractures.. Clin Rev Bone Miner Metab 2018 Dec;16(4):131-141.
              doi: 10.1007/s12018-018-9254-zpubmed: 30804713google scholar: lookup
            12. Roth SP, Schubert S, Scheibe P, Groß C, Brehm W, Burk J. Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix.. Cell Transplant 2018 Oct;27(10):1434-1450.
              doi: 10.1177/0963689718792203pubmed: 30251565google scholar: lookup
            13. Roth SP, Glauche SM, Plenge A, Erbe I, Heller S, Burk J. Automated freeze-thaw cycles for decellularization of tendon tissue - a pilot study.. BMC Biotechnol 2017 Feb 14;17(1):13.
              doi: 10.1186/s12896-017-0329-6pubmed: 28193263google scholar: lookup
            14. Lovati AB, Bottagisio M, Moretti M. Decellularized and Engineered Tendons as Biological Substitutes: A Critical Review.. Stem Cells Int 2016;2016:7276150.
              doi: 10.1155/2016/7276150pubmed: 26880985google scholar: lookup
            15. Gomiero C, Bertolutti G, Martinello T, Van Bruaene N, Broeckx SY, Patruno M, Spaas JH. Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology.. Vet Res Commun 2016 Mar;40(1):39-48.
              doi: 10.1007/s11259-016-9652-ypubmed: 26757735google scholar: lookup
            16. Schulze-Tanzil G, Al-Sadi O, Ertel W, Lohan A. Decellularized tendon extracellular matrix-a valuable approach for tendon reconstruction?. Cells 2012 Nov 5;1(4):1010-28.
              doi: 10.3390/cells1041010pubmed: 24710540google scholar: lookup
            Subscribe to our newsletter
            Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.