Comparison of the lipoprotein profiles obtained from rat, bovine, horse, dog, rabbit and pig serum by a new two-step ultracentrifugal gradient procedure.
Abstract: A new two-step gradient technique has been used in the separation of the different classes of lipoproteins from the serum of cows, horses, dogs, pigs, rabbits and rats. Total lipoproteins were first isolated at d 1.21 then floated through a d 1.006 to d 1.21 gradient. Collection by mean of a gradient fractionator provided directly comparable lipoprotein profiles, allowed the determination of the exact density range of each lipoprotein class and the fraction by fraction analysis of composition. Cholesterol and apo AI recoveries were high. Horse, dog, rabbit and pig exhibited three distinct lipoprotein classes: VLDL, LDL and HDL. LDL were polydisperse in the pig (three components), light in the rabbit and scarce in the horse. In the Sprague-Dawley rat, LDL could not be individualized from HDL. In the bovine, LDL overlapped with a light form of HDL. Although AI was the main apoprotein in the HDL of all species, it ranged in proportion from 35% in the rat to 75% in the bovine. Apo AII was dimeric in the dog, as already known, but also in the horse, rabbit and bovine (MW:17,000) and in the pig MW:13,000). Apo AIV was present in the heavier HDL of all species. Rabbit, horse and pig HDL contained only one species of apo C which, in the pig was identified as apo CII.
Publication Date: 1986-01-01 PubMed ID: 3720292DOI: 10.1016/0305-0491(86)90275-0Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research article discusses a study where a novel two-step gradient technique was employed to segregate different classes of lipoproteins from the serum of a variety of animals including cows, horses, dogs, pigs, rabbits, and rats.
Methodology
- The researchers used a two-step gradient technique to separate lipoprotein classes from the serum of multiple species.
- Initially, total lipoproteins were segregated at density 1.21. These isolated lipoproteins were then filtered through a density gradient ranging from 1.006 to 1.21.
- A gradient fractionator was used to collect the samples, allowing direct comparison of lipoprotein profiles and enabling the determination of precise density range of each lipoprotein class, as well as a fraction-by-fraction composition analysis.
Results
- The technique led to high recoveries of cholesterol and Apo AI.
- Three distinct classes of lipoproteins were found in horses, dogs, rabbits, and pigs, namely VLDL, LDL, and HDL.
- The LDL class was differentiated into polydisperse components in pig serum, while appearing light in rabbit serum and being sparse in horse serum.
- In rats, LDL couldn’t be individualized from HDL. In bovine serum, LDL overlapped with a lighter form of HDL.
Distinct Properties of Lipoproteins
- The composition of Apo AI, a primary apoprotein in HDL, varied across species, ranging from 35% in rat serum to 75% in bovine serum.
- Apo AII was found to be dimeric in dogs, as it was in horses, rabbits, bovines, and pigs.
- Apo AIV was present in the heavier HDL across all species.
- Only one type of apo C was present in rabbit, horse, and pig HDL. In pigs, this was identified as apo CII.
Overall, the study showcases the potential of the novel two-step gradient technique in separating and analyzing different classes of lipoproteins from a variety of animal serums. This technique could be crucial for veterinary biochemical research and comparative animal studies.
Cite This Article
APA
Hollanders B, Mougin A, N'Diaye F, Hentz E, Aude X, Girard A.
(1986).
Comparison of the lipoprotein profiles obtained from rat, bovine, horse, dog, rabbit and pig serum by a new two-step ultracentrifugal gradient procedure.
Comp Biochem Physiol B, 84(1), 83-89.
https://doi.org/10.1016/0305-0491(86)90275-0 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Apolipoproteins / blood
- Cattle
- Chromatography, Gel
- Dogs
- Horses
- Lipoproteins / blood
- Lipoproteins / isolation & purification
- Lipoproteins, HDL / blood
- Lipoproteins, LDL / blood
- Lipoproteins, VLDL / blood
- Rabbits
- Rats
- Species Specificity
- Swine
- Ultracentrifugation
Citations
This article has been cited 3 times.- Zhang N, Ye X, Wu Y, Huang Z, Gu X, Cai Q, Shen X, Jiang H, Ding H. Determination of the Mutant Selection Window and Evaluation of the Killing of Mycoplasma gallisepticum by Danofloxacin, Doxycycline, Tilmicosin, Tylvalosin and Valnemulin. PLoS One 2017;12(1):e0169134.
- Bigazzi F, Adorni MP, Puntoni M, Sbrana F, Lionetti V, Pino BD, Favari E, Recchia FA, Bernini F, Sampietro T. Analysis of Serum Cholesterol Efflux Capacity in a Minipig Model of Nonischemic Heart Failure. J Atheroscler Thromb 2017 Aug 1;24(8):853-862.
- Carpintero R, Piñeiro M, Andrés M, Iturralde M, Alava MA, Heegaard PM, Jobert JL, Madec F, Lampreave F. The concentration of apolipoprotein A-I decreases during experimentally induced acute-phase processes in pigs. Infect Immun 2005 May;73(5):3184-7.
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