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Comparison of the methods for platelet rich plasma preparation in horses.
Abstract: Platelet rich plasma (PRP) is popularly used in the horse industry to enhance regeneration of tissue injury that has limitation of blood supply. This study aimed to compare the methods for platelet rich plasma preparation since they has not been established yet. Blood was collected from six horses and platelets were concentrated by three different methods (2-step centrifugation, separated centrifugation and separated centrifugation using histopaque). Concentrated blood was analyzed using Advia hematology systems. In the result, separated centrifugation with histopaque showed the significantly lower number of red blood cells than other groups. The 2-step centrifugation showed the significantly higher number of white blood cells than other groups, while it contained the highest concentration of red blood cells among three groups. In the 2-step centrifugation, separated centrifugation and separated centrifugation with histopaque, platelets were concentrated 4.5, 5.3 and 5.6 times, respectively. And no significant difference of the platelet concentration between the three groups was found. This study demonstrated that separated centrifugation using histopaque was the best method for platelet rich plasma preparation because of the proper amount of platelets and the separation of red blood cells from platelet rich plasma.
Publication Date: 2018-08-18 PubMed ID: 30147942PubMed Central: PMC6098823DOI: 10.1186/s40781-018-0178-4Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This research article explores and compares three different methods used to prepare platelet rich plasma (PRP) from horse blood. It determines that the most effective method is the separated centrifugation using histopaque, as it provides an adequate amount of platelets and effectively separates the red blood cells from the platelet rich plasma.
Objective and Methodology
- PRP (Platelet Rich Plasma) is widely utilized in the equine industry mainly as a treatment method meant to boost tissue injury repair, especially in areas that lack good blood supply.
- The main goal of this research was to validate and compare the effectiveness of different techniques used for PRP preparation in horses, as the ideal method is yet to be established.
- Blood samples were drawn from six horses for the study and then had their platelets concentrated using three different techniques: 2-step centrifugation, separated centrifugation, and histopaque-separate centrifugation.
- The resultant concentrated blood was subjected to an analysis using Advia hematology systems to check for results.
Findings
- The method of separated centrifugation using histopaque demonstrated a significantly lower count of red blood cells compared to the other two methods.
- 2-step centrifugation had a significantly higher count of white blood cells as compared to the other two methods, plus it contained the highest concentration of red blood cells among the three.
- The concentration of platelets using 2-step centrifugation, separated centrifugation, and histopaque-separated centrifugation increased by factors of 4.5, 5.3, and 5.6 times, respectively.
- No considerable difference in platelet concentration was observed across the three application methods.
Conclusion
- The results of this research ascertain that the separated centrifugation using histopaque is the best technique for the preparation of PRP.
- This is primarily because this method not only ensures the correct concentration of platelets, but also effectively separates the red blood cells in the platelet rich plasma sample.
Cite This Article
APA
Lee EB, Kim JW, Seo JP.
(2018).
Comparison of the methods for platelet rich plasma preparation in horses.
J Anim Sci Technol, 60, 20.
https://doi.org/10.1186/s40781-018-0178-4 Publication
Researcher Affiliations
- College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju-City, Jejudo 63243 Republic of Korea.
- College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju-City, Jejudo 63243 Republic of Korea.
- College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju-City, Jejudo 63243 Republic of Korea.
Conflict of Interest Statement
The experimental procedure and methods were approved by the Experimental Animal Committee of Veterinary Medicine of Jeju National University, Jeju, Republic of Korea.Not applicable.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
References
This article includes 13 references
- Hessel LN, Bosch G, van Weeren PR, Ionita JC. Equine autologous platelet concentrates: A comparative study between different available systems.. Equine Vet J 2015 May;47(3):319-25.
- Textor JA, Norris JW, Tablin F. Effects of preparation method, shear force, and exposure to collagen on release of growth factors from equine platelet-rich plasma.. Am J Vet Res 2011 Feb;72(2):271-8.
- Argüelles D, Carmona JU, Pastor J, Iborra A, Viñals L, Martínez P, Bach E, Prades M. Evaluation of single and double centrifugation tube methods for concentrating equine platelets.. Res Vet Sci 2006 Oct;81(2):237-45.
- Fontenot RL, Sink CA, Werre SR, Weinstein NM, Dahlgren LA. Simple tube centrifugation for processing platelet-rich plasma in the horse.. Can Vet J 2012 Dec;53(12):1266-72.
- Rutkowski JL, Thomas JM, Bering CL, Speicher JL, Radio NM, Smith DM, Johnson DA. Analysis of a rapid, simple, and inexpensive technique used to obtain platelet-rich plasma for use in clinical practice.. J Oral Implantol 2008;34(1):25-33.
- Carr BJ, Canapp SO Jr, Mason DR, Cox C, Hess T. Canine Platelet-Rich Plasma Systems: A Prospective Analysis.. Front Vet Sci 2015;2:73.
- Maia L, Souza MV, Ribeiro Jr BS, J.I., de Oliveira AC, Silveira Alves GE, dos Anjos Benjamin L, Robaina Sancler Silvaa YF, Zandi BM, do Carmo Lopes Moreira J. Platelet-rich plasma in the treatment of induced tendinopathy in horses: histologic evaluation. J Equine Vet Sci 2009;29:618–626.
- Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF).. Trends Biotechnol 2009 Mar;27(3):158-67.
- Boswell SG, Schnabel LV, Mohammed HO, Sundman EA, Minas T, Fortier LA. Increasing platelet concentrations in leukocyte-reduced platelet-rich plasma decrease collagen gene synthesis in tendons.. Am J Sports Med 2014 Jan;42(1):42-9.
- Perez AG, Lana JF, Rodrigues AA, Luzo AC, Belangero WD, Santana MH. Relevant aspects of centrifugation step in the preparation of platelet-rich plasma.. ISRN Hematol 2014;2014:176060.
- Sundman EA, Cole BJ, Fortier LA. Growth factor and catabolic cytokine concentrations are influenced by the cellular composition of platelet-rich plasma.. Am J Sports Med 2011 Oct;39(10):2135-40.
- McCarrel T, Fortier L. Temporal growth factor release from platelet-rich plasma, trehalose lyophilized platelets, and bone marrow aspirate and their effect on tendon and ligament gene expression.. J Orthop Res 2009 Aug;27(8):1033-42.
- Yoshida R, Murray MM. Peripheral blood mononuclear cells enhance the anabolic effects of platelet-rich plasma on anterior cruciate ligament fibroblasts.. J Orthop Res 2013 Jan;31(1):29-34.
Citations
This article has been cited 7 times.- Mayet A, Zablotski Y, Roth SP, Brehm W, Troillet A. Systematic review and meta-analysis of positive long-term effects after intra-articular administration of orthobiologic therapeutics in horses with naturally occurring osteoarthritis. Front Vet Sci 2023;10:1125695.
- Sharun K, Chandran D, Manjusha KM, Mankuzhy PD, Kumar R, Pawde AM, Dhama K, El-Husseiny HM, Amarpal. Advances and prospects of platelet-rich plasma therapy in veterinary ophthalmology. Vet Res Commun 2023 Sep;47(3):1031-1045.
- Segabinazzi LGTM, Podico G, Rosser MF, Nanjappa SG, Alvarenga MA, Canisso IF. Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma. Animals (Basel) 2021 May 21;11(6).
- Fukuda K, Kuwano A, Kuroda T, Tamura N, Mita H, Okada Y, Kasashima Y. Optimal double-spin method for maximizing the concentration of platelets in equine platelet-rich plasma. J Equine Sci 2020;31(4):105-111.
- Carmona JU, López C, Argüelles D. Addressing Heterogeneity in Equine PRP Therapies: A Scoping Review of Methods, Evidence, and Commercial Validation. Animals (Basel) 2025 Dec 13;15(24).
- Bernardini C, Romagnoli N, Casalini I, Turba ME, Spadari A, Forni M, Gentilini F. Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate. Int J Vet Sci Med 2024;12(1):71-80.
- Gueguen L, Henry S, Delbos M, Lemasson A, Hausberger M. Selected Acoustic Frequencies Have a Positive Impact on Behavioural and Physiological Welfare Indicators in Thoroughbred Racehorses. Animals (Basel) 2023 Sep 20;13(18).
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