[Complement fixation reaction studies in rhinopneumonitis of horses].

The study explains that the complement binding reaction (CBR) is a quick, reliable method for diagnosing rhinopneumonitis in horses. Cell cultures from pig kidneys were used as antigens for this test, and the non-diluted antigens detected specific immune responses most effectively. The researchers tested the CBR’s specificity using various types of serums and antigens, and they found that most horses who had recovered from rhinopneumonitis had complement binding antibodies in their blood.

Usage of Complement Binding Reaction (CBR) for Diagnosis

• The researchers established that the complement binding reaction (CBR) is a suitable method for diagnosing rhinopneumonitis in horses.
• A very quick process, CBR utilizes an antigen, in this case derived from pig kidney cell cultures, to elicit a response from the immune system.
• The researchers noted that the antigen lots they tested did not have anti-complementary properties, meaning they didn’t interfere with the body’s immune response.

Effectiveness of Non-Diluted Antigens

• The research indicates that the highest complement binding activity was observed in non-diluted antigen. This means that a greater concentration of the antigen is more effective at provoking an immune response.
• Specific antibodies in immune serums were discovered using this non-diluted antigen, demonstrating its effectiveness.

Testing of CBR Specificity

• The CBR’s specificity was tested by using homologous and heterologous serums and antigens. This ensures that the reactions observed were specific to the disease being tested.

Presence of Complement Binding Antibodies in Recovered Horses

• The complement binding antibodies were discovered in varying concentrations, from 1:10 to 1:320, and in some rare cases, even to 1:640, in the serums of 255 horses that had recovered from the disease.
• The blood samples these results were obtained from were those of horses in studs that had a confirmed diagnosis of rhinopneumonitis by clinical observation, virus neutralizing reaction and virus isolation.
• The research concluded that in almost all the horses studied, neutralizing and complement binding antibodies were established at the same time.