Composition of neutral lipids from erythrocytes of common mammals.
Abstract: The neutral lipids of the erythrocytes were investigated in several common mammals: cow, dog, goat, horse, pig, rabbit, rat, and sheep. Cholesterol content was determined by gas-liquid, thin-layer, and column chromatography, the last in conjunction with the IR spectrophotometry. The three methods yielded similar results. In every species investigated, cholesterol was the major neutral lipid; cholesteryl esters, triglycerides, and free fatty acids were detected only in trace amounts. It is concluded that these substances may have been contaminants from plasma lipoproteins or leukocytes rather than true constituents of the erythrocyte. In the erythrocytes of all species, cholesterol content was close to 30% of the total lipids extracted from the cells, and the molar ratio of cholesterol to phospholipid was approximately one. The significance of the data is discussed in relation to current concepts of the structure of the cell membrane involving cholesterol-polar lipid complexes.
Publication Date: 1967-07-01 PubMed ID: 6033604
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- Journal Article
Summary
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This research studied the makeup of neutral lipids in red blood cells, or erythrocytes, of several common mammals. They found that cholesterol was the main neutral lipid present, with other substances detected likely contaminants instead of genuine constituents of the cell.
Study Methodology
- The researchers examined neutral lipids of erythrocytes in several mammals, including cows, dogs, goats, horses, pigs, rabbits, rats, and sheep.
- The main focus of the study was on cholesterol and other lipid content in these mammals’ erythrocytes.
- The researchers employed three different methods to determine the cholesterol content: gas-liquid, thin-layer, and column chromatography, with the former being used in conjunction with infrared (IR) spectrophotometry.
- All three analytical methods yielded similar results, verifying the cholesterol content of the cells.
Study Findings
- Cholesterol was found to be the major neutral lipid in the erythrocytes of all mammals examined.
- Other substances like cholesteryl esters, triglycerides, and free fatty acids were detected, but only in trace amounts.
- The researchers concluded that the detected trace amounts are possibly contaminants from plasma lipoproteins or leukocytes, suggesting they may not be true constituents of the erythrocyte.
- In the studied erythrocytes, the cholesterol content was approximately 30% of the total lipids extracted from the cells.
- The molar ratio of cholesterol to phospholipid in the erythrocytes was around one.
Interpretation of Results and Implications
- The results of the study contribute to the understanding of the cell membrane structure and the role of cholesterol therein.
- The findings are discussed within the context of current cellular structure concepts that involve cholesterol-polar lipid complexes.
- The observation that the major lipid in erythrocytes is cholesterol, suggests its crucial role in maintaining cell structure and function across different mammalian species.
Cite This Article
APA
Nelson GJ.
(1967).
Composition of neutral lipids from erythrocytes of common mammals.
J Lipid Res, 8(4), 374-379.
Publication
Researcher Affiliations
MeSH Terms
- Animals
- Cattle
- Cell Membrane / analysis
- Cholesterol / analysis
- Chromatography
- Dogs
- Erythrocytes / analysis
- Fatty Acids / analysis
- Goats
- Horses
- Lipids / analysis
- Phosphorus / analysis
- Rabbits
- Rats
- Sheep
- Spectrum Analysis
- Swine
- Triglycerides / analysis
Citations
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