Comprehensive qualification and quantification of triacylglycerols with specific fatty acid chain composition in horse adipose tissue, human plasma and liver tissue.
Abstract: High levels of triacylglycerols (TGs) have been linked to cardiovascular disease and liver diseases. Comprehensively analyzing TGs is helpful to understand the TGs functions in these diseases. However, due to the existence of a large number of isomers TGs and the lack of commercial standards, precise analysis of individual triacylglycerol (TG) with specific fatty acid chain composition is full of challenge. In this work, ultra-performance liquid chromatography (UPLC) coupled with electrospray ionization (ESI) mass spectrometry (MS) were employed for comprehensive qualification and quantification of TGs with specific fatty acid chain composition in horse adipose tissue, human plasma and liver tissues including hepatocellular carcinoma (HCC) and para-carcinoma tissues. Multiple MS detection modes from QTRAP MS and FT-ICR MS were utilized, and hundreds of TG species (including many oxidized TG species) with their specific fatty acid chain compositions have been qualified and quantified. The isomer TGs interference, the isobaric interference, and oxidized TG species interference were firstly indicated. Several isomer TGs, for example, 18:1/20:1/18:2 TG and 20:3/18:1/18:0 TG, which were all 56:4 TG, demonstrated different trends in HCC tissue compared with para-carcinoma tissue, which showed the importance of analysis of TG with specific fatty acid chain composition. In addition, 10 TGs with the degree of unsaturation beyond three were significantly decreased, while 16:0/17:0/18:0 TG, no double bond, was significantly increased in the HCC tissue, which firstly revealed aberrant specific TG metabolism in HCC. This is a systematic report about comprehensive analysis of TGs by UPLC-ESI-MS, which is of significance for accurate analysis of these lipids.
Copyright © 2017 Elsevier B.V. All rights reserved.
Publication Date: 2017-05-19 PubMed ID: 28602296DOI: 10.1016/j.talanta.2017.05.042Google Scholar: Lookup
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Summary
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The research paper presents a detailed investigation into the comprehensive analysis of triacylglycerols with specific fatty acid chain composition in horse adipose tissue, human plasma, and liver tissues. It highlights the importance of understanding triacylglycerols functions in cardiovascular and liver diseases and the challenges faced due to the high variety of isomers and lack of commercial standards.
Application of Ultra-Performance Liquid Chromatography and Mass Spectrometry
- This research employed two advanced techniques: ultra-performance liquid chromatography (UPLC) and electrospray ionization (ESI) mass spectrometry (MS) to comprehensively qualify and quantify triacylglycerols (TGs) with specific fatty acid chain composition.
- The study focused on various types of tissues including horse adipose tissue, human plasma, and specifically, liver tissues. These liver tissues consisted of both hepatocellular carcinoma (HCC) – a common type of primary liver cancer, and the tissues surrounding the carcinoma (para-carcinoma tissues).
Multiple MS Detection Modes and Interferences
- The researchers utilized multiple MS detection modes from QTRAP MS and FT-ICR MS.
- Hundreds of TG species, including many oxidized TG species, were qualified and quantified, with their specific fatty acid chain compositions.
- The study indicated several interferences for the first time, such as isomer TGs interference, isobaric interference, and oxidized TG species interference.
Significance of Specific Fatty Acid Chain Composition
- The study showed that different isomer TGs, with the same overall molecular composition but different fatty acid chains, demonstrated different trends in HCC tissue compared with para-carcinoma tissue, thus emphasizing the importance of analysing TGs considering their specific fatty acid chain composition.
- The research discovered certain specific TG metabolism anomalies in HCC. Ten TGs with a degree of unsaturation above three were significantly decreased, while 16:0/17:0/18:0 TG, which has no double bond, was significantly increased in HCC tissue.
Concluding Importance of the Research
- This study can be considered a systematic report about in-depth analysis of TGs using UPLC-ESI-MS.
- It provides significant implications for the accurate analysis of these types of lipids, which could potentially influence the future diagnostics and therapeutics strategies for cardiovascular and liver diseases.
Cite This Article
APA
Guan M, Dai D, Li L, Wei J, Yang H, Li S, Zhang Y, Lin Y, Xiong S, Zhao Z.
(2017).
Comprehensive qualification and quantification of triacylglycerols with specific fatty acid chain composition in horse adipose tissue, human plasma and liver tissue.
Talanta, 172, 206-214.
https://doi.org/10.1016/j.talanta.2017.05.042 Publication
Researcher Affiliations
- Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing, China; Graduate School, University of Chinese Academy of Sciences, Beijing, China.
- Nanjing Sanhome Pharmaceutical Co., Ltd, Nanjing, China.
- Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing, China; Graduate School, University of Chinese Academy of Sciences, Beijing, China.
- Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing, China.
- Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing, China; Graduate School, University of Chinese Academy of Sciences, Beijing, China.
- Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing, China; Graduate School, University of Chinese Academy of Sciences, Beijing, China.
- Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing, China.
- Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing, China.
- Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing, China.
- Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing, China; Graduate School, University of Chinese Academy of Sciences, Beijing, China. Electronic address: zhenwenzhao@iccas.ac.cn.
MeSH Terms
- Adipose Tissue / chemistry
- Animals
- Blood Chemical Analysis / methods
- Fatty Acids / chemistry
- Horses
- Humans
- Liver / chemistry
- Spectrometry, Mass, Electrospray Ionization
- Triglycerides / analysis
- Triglycerides / blood
- Triglycerides / chemistry
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