Correction of equine severe combined immunodeficiency by bone marrow transplantation.
Abstract: A 32-day-old horse with severe combined immunodeficiency was transplanted with equine bone marrow cells in an attempt to establish immunologic responsiveness. A histocompatible, mixed-leukocyte-culture-nonreactive, sex-matched, full sibling was used as the donor. Recipient total lymphocyte count, T and B lymphocyte numbers, and response of peripheral blood mononuclear cells to phytolectin stimulation increased by 14 days following transplantation. Circulating lymphocytes exceeded 1000 cells/microliter blood by 40 days posttransplantation, and by 170 days following transplantation, T and B lymphocyte numbers had reached normal values. The foal demonstrated significant primary and secondary antibody responses when immunized with bacteriophage phi X 174 at 100 and 142 days posttransplantation. Concentrations of IgG and IgM remained within the normal range following cessation of i.v. plasma therapy 156 days after transplantation. More than 300 days following transplantation, the foal remains healthy and is growing normally. At no time during the posttransplant period was there detectable evidence of graft-versus-host disease.
Publication Date: 1986-07-01 PubMed ID: 3523877DOI: 10.1097/00007890-198607000-00003Google Scholar: Lookup
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- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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This study details how a horse with a severe combined immunodeficiency was treated successfully through a bone marrow transplant. The transplant led to an increase in immune responsiveness, with the horse showing effective antibody responses when immunized post-transplant.
Research Objectives and Methodology
- The primary goal of the research was to establish immunological responsiveness in a horse suffering from severe combined immunodeficiency. The method chosen to achieve this was a bone marrow transplant.
- The marrow donor was a histocompatible, full-sibling of the recipient horse, which reduces chances of rejection or complications related to the transplant.
- Histocompatibility was ascertained through a mixed-leukocyte-culture-nonreactive test, an important step to ensure that the recipient’s immune system does not attack the transplanted cells.
Transplantation and Post-Transplant Observations
- Within 14 days of the bone marrow transplant, there was significant improvement in the horse’s immune system. This was indicated by an increased total lymphocyte count, as well as increased numbers of T and B lymphocytes which are key components of the body’s immune response.
- 40 days post-transplantation, the circulating lymphocyte count had exceeded 1000 cells/microliter of blood, and by 170 days, both T and B lymphocyte numbers had reached normal values.
- The horse exhibited remarkable primary and secondary antibody responses post-transplant upon immunization with bacteriophage phi X 174.
Long-Term Post-Transplant Results
- Concentrations of immunoglobulins IgG and IgM (essential for the body’s immunity) stayed within the normal range even after plasma therapy stopped 156 days post-transplantation.
- More than 300 days following the transplant, the horse was healthy and growing normally.
- Crucially, there was no detectable evidence of graft-versus-host disease (a condition where donor’s cells attack recipients tissue) at any point during the post-transplant period, indicating the procedure was a success.
In summary, the study showcased a successful case of immunologic recovery in a horse afflicted with severe combined immunodeficiency using bone marrow transplantation. Demonstrating the potential of this treatment for horses with this immunodeficiency brings new hope for such cases in equine veterinary medicine.
Cite This Article
APA
Bue CM, Davis WC, Magnuson NS, Mottironi VD, Ochs HD, Wyatt CR, Perryman LE.
(1986).
Correction of equine severe combined immunodeficiency by bone marrow transplantation.
Transplantation, 42(1), 14-19.
https://doi.org/10.1097/00007890-198607000-00003 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Bone Marrow Transplantation
- Female
- Horses
- Immunologic Deficiency Syndromes / therapy
- Male
- Stem Cell Transplantation
- Time Factors
Grant Funding
- HD 08886 / NICHD NIH HHS
Citations
This article has been cited 3 times.- Bauer TR Jr, Adler RL, Hickstein DD. Potential large animal models for gene therapy of human genetic diseases of immune and blood cell systems. ILAR J 2009;50(2):168-86.
- Mealey RH, Fraser DG, Oaks JL, Cantor GH, McGuire TC. Immune reconstitution prevents continuous equine infectious anemia virus replication in an Arabian foal with severe combined immunodeficiency: lessons for control of lentiviruses. Clin Immunol 2001 Nov;101(2):237-47.
- Bjorneby JM, Leach DR, Perryman LE. Persistent cryptosporidiosis in horses with severe combined immunodeficiency. Infect Immun 1991 Oct;59(10):3823-6.
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