Cultivation of Schwann cells from fresh and non-fresh adult equine peripheral nerves.
Abstract: Over the past 25 years, acquired equine polyneuropathy (AEP) has emerged as a neurological disease in Scandinavian horses. This condition is characterized by histopathological features including the presence of Schwann cell (SC) inclusions. Cultivated equine SCs would serve as a valuable resource for investigations of factors triggering this Schwannopathy. Ideally, cells should be sampled for cultivation from fresh nerves immediately after death of the animal, however the availability of fresh material is limited, due to the inconsistent case load and the inherent technical and practical challenges to collection of samples in the field. This study aimed to cultivate SCs from adult equine peripheral nerves and assess their ability to survive in sampled nerve material over time to simulate harvesting of SCs in field situations. Methods: Peripheral nerves from five non-neurological horses were used. After euthanasia, both fresh and non-fresh nerve samples were harvested from each horse. Flow cytometry was employed to confirm the cellular identity and to determine the SC purity. Results: The results revealed successful establishment of SC cultures from adult equine peripheral nerves, with the potential to achieve high SC purity from both fresh and non-fresh nerve samples. Conclusions: While most SC isolation methods focus on harvest of cells from fresh nerve materials from laboratory animals, our approach highlights the possibility of utilizing SC cultures from field-harvested and transported nerve samples from horses. Conclusions: We describe a method for isolating SCs with high purity from both fresh and non-fresh peripheral nerves of adult horses.
Copyright © 2024. Published by Elsevier B.V.
Publication Date: 2024-01-03 PubMed ID: 38181868DOI: 10.1016/j.jneumeth.2023.110054Google Scholar: Lookup
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- Journal Article
Summary
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This research studied methods of cultivating Schwann cells from both fresh and non-fresh adult equine peripheral nerves. These cells are invaluable in the ongoing investigation into a neurological disease affecting horses. The study showed that it is feasible to establish high purity Schwann cell cultures from both fresh and non-fresh nerve samples.
Objective of the Research
- The focus of this study was acquired equine polyneuropathy (AEP), a neurological disease seen in Scandinavian horses over the past 25 years. The disease exhibits several histopathological features, including the presence of Schwann cell (SC) inclusions.
- The research aimed to cultivate Schwann cells from adult equine peripheral nerves and assess their viability over time. This was done in order to simulate the gathering of Schwann cells in real-life scenarios where fresh samples may not be readily available.
Methodology
- Peripheral nerves were collected from five non-neurological horses post-euthanasia. Both fresh and non-fresh nerve samples were taken from each animal.
- Flow cytometry, a technique used to measure and analyze physical and chemical characteristics of cells, was used to verify the cellular identity and determine the purity of the Schwann cells.
Results
- The study was successful in establishing Schwann cell cultures from adult equine peripheral nerves. High purity Schwann cell cultures could be obtained from both fresh and non-fresh nerve samples.
Conclusion
- Most Schwann cell isolation strategies only consider fresh nerve material from lab animals, but the method detailed in this study indicates that it’s possible to cultivate Schwann cells from nerve samples that have been harvested from horses in the field and transported.
- The research provides a new technique of isolating Schwann cells with high purity from adult horse nerves, regardless of whether the nerve samples are fresh or non-fresh.
Cite This Article
APA
Kvigstad EF, Øverland IK, Skedsmo FS, Jäderlund KH, Gröndahl G, Hanche-Olsen S, Gunnes G.
(2024).
Cultivation of Schwann cells from fresh and non-fresh adult equine peripheral nerves.
J Neurosci Methods, 110054.
https://doi.org/10.1016/j.jneumeth.2023.110054 Publication
Researcher Affiliations
- Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, Norway.
- Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, Norway.
- Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, Norway.
- Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oluf Thesensvei 24/30, 1433 Ås, Norway.
- Department of Animal Health and Microbial Strategies, National Veterinary Institute, 75189 Uppsala, Sweden.
- Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oluf Thesensvei 24/30, 1433 Ås, Norway.
- Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, Norway. Electronic address: gjermund.gunnes@nmbu.no.
Conflict of Interest Statement
Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Siv Hanche-Olsen reports was provided by Swedish-Norwegian Foundation for Equine Research, supportet by the Reseach Concil of Norway Competing interests The authors declare that they have no competing interests.
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