Cytogenetic localization of 44 new coding sequences in the horse.
Abstract: The purpose of this study was to increase the number of genes assigned by in situ hybridization to equine chromosomes and thus the number of links for comparative mapping with other species. Forty-four new sequences were added to the horse cytogenetic map by FISH mapping of BAC clones containing genes (35) or ESTs (9). Three approaches were developed: use of horse BAC clones screened with (i) horse EST primers, (ii) interspecific consensus intraexonic primers, and (iii) use of goat BAC containing genes previously localized on goat chromosomes. Present data suggest that the second approach is the most promising. A total of 46 segments containing one or several genes could be compared, among which 40 loci could be included in 16 synteny groups between human and horse, displaying one ordered segment and several breaking points along chromosomes. All single BAC localizations confirm the most recent mapping data. Twenty-six out of 31 chromosomes now contain a gene mapped by in situ hybridization, and 14 new arm-to-arm segment homologies were revealed.
Publication Date: 2000-12-29 PubMed ID: 11130977DOI: 10.1007/s003350010206Google Scholar: Lookup
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- Journal Article
Summary
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The research is centered on expanding the number of genes assigned to horse chromosomes and thus strengthening the ties for comparative mapping with other species. It involves the use of various technologies and strategies to map 44 new sequences onto the horse’s genetic map.
Objective and Strategies
- The central focus of the study was to increase the number of genes assigned to equine (horse) chromosomes. This is significant for enhancing comparative mapping across different species. This process is referred to as cytogenetic localization.
- In this research, 44 new sequences were added to the horse’s genetic map through FISH (Fluorescence In Situ Hybridization) mapping of BAC (Bacterial Artificial Chromosome) clones with genes or ESTs (Expressed Sequence Tags). These include 35 genes and 9 ESTs.
- Three distinctive approaches were used for this mapping process. The first involved the use of horse BAC clones screened with horse EST primers. The second deployed interspecific consensus intraexonic primers, and the third relied on goat BAC containing genes previously localized on goat chromosomes.
Findings and Implications
- The research found that the second approach, using interspecific consensus intraexonic primers, appears to be the most promising for the cytogenetic localization of new coding sequences in horses.
- They were able to compare 46 segments containing either one or many genes. Among these, 40 loci could be grouped into 16 synteny groups between human and horse, exhibiting one ordered segment and several breaking points along chromosomes.
- Every single BAC localization bolstered the most recent mapping data, indicating a strong methodological consistency.
- The research concluded that now 26 out of 31 horse chromosomes contain a gene mapped by in situ hybridization. This is a notable advancement in the field of cytogenetics, particularly concerning equine species.
- The study also uncovered 14 new arm-to-arm segment homologies. These, along with other findings, will contribute significantly to the further understanding of horse genetics and also potentially other species.
Cite This Article
APA
Godard S, Vaiman A, Schibler L, Mariat D, Vaiman D, Cribiu EP, Guérin G.
(2000).
Cytogenetic localization of 44 new coding sequences in the horse.
Mamm Genome, 11(12), 1093-1097.
https://doi.org/10.1007/s003350010206 Publication
Researcher Affiliations
- INRA, Centre de Recherches de Jouy, Département de Génétique Animale, Jouy-en-Josas, France.
MeSH Terms
- Animals
- Chromosome Mapping
- Chromosomes, Artificial, Bacterial
- Expressed Sequence Tags
- Horses / genetics
- In Situ Hybridization, Fluorescence
Citations
This article has been cited 6 times.- Finno CJ, Bannasch DL. Applied equine genetics.. Equine Vet J 2014 Sep;46(5):538-44.
- Raudsepp T, Gustafson-Seabury A, Durkin K, Wagner ML, Goh G, Seabury CM, Brinkmeyer-Langford C, Lee EJ, Agarwala R, Stallknecht-Rice E, Schäffer AA, Skow LC, Tozaki T, Yasue H, Penedo MC, Lyons LA, Khazanehdari KA, Binns MM, MacLeod JN, Distl O, Guérin G, Leeb T, Mickelson JR, Chowdhary BP. A 4,103 marker integrated physical and comparative map of the horse genome.. Cytogenet Genome Res 2008;122(1):28-36.
- Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence.. Chromosome Res 2008;16(1):109-27.
- Brinkmeyer-Langford C, Raudsepp T, Lee EJ, Goh G, Schäffer AA, Agarwala R, Wagner ML, Tozaki T, Skow LC, Womack JE, Mickelson JR, Chowdhary BP. A high-resolution physical map of equine homologs of HSA19 shows divergent evolution compared with other mammals.. Mamm Genome 2005 Aug;16(8):631-49.
- Chowdhary BP, Raudsepp T, Kata SR, Goh G, Millon LV, Allan V, Piumi F, Guérin G, Swinburne J, Binns M, Lear TL, Mickelson J, Murray J, Antczak DF, Womack JE, Skow LC. The first-generation whole-genome radiation hybrid map in the horse identifies conserved segments in human and mouse genomes.. Genome Res 2003 Apr;13(4):742-51.
- Raudsepp T, Chowdhary BP. Correspondence of human chromosomes 9, 12, 15, 16, 19 and 20 with donkey chromosomes refines homology between horse and donkey karyotypes.. Chromosome Res 2001;9(8):623-9.
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