Cytokine and chemokine gene expression of IL-1beta stimulated equine articular chondrocytes.
Abstract: To evaluate mRNA expression of several proinflammatory and anti-inflammatory cytokines and chemokines in equine unstimulated and interleukin-1beta (IL-1beta)-stimulated chondrocytes. Methods: In vitro experiment using equine chondrocyte cultures. Methods: Whole articular cartilage from metacarpophalangeal joints (n=5 horses; 10 fetlocks). Methods: Chondrocyte monolayer cultures were established from digested adult equine articular cartilage and stimulated with 5 ng/mL of recombinant human IL-1beta. RNA was extracted from the cells 24 hours after stimulation. IL-1beta, IL-4, IL-6, IL-8, tumor necrosis factor-alpha (TNF-alpha), and ubiquitin (house keeping gene) mRNA expression were investigated by real-time RT-PCR. Results: IL-1beta, IL-6, and IL-8 mRNA were expressed in unstimulated chondrocytes from macroscopically normal joints and were significantly up-regulated after stimulation (5/5 horses). IL-4 mRNA was not detected in any samples (0/5 horses). TNF-alpha mRNA, by comparison, was expressed in 2/5 unstimulated samples and in all stimulated samples but a considerable sample variation in response to IL-1beta stimulation was observed. Conclusions: Equine chondrocytes express mRNA for several proinflammatory cytokines and chemokines and IL-1beta modulates their expression. Conclusions: Chondrocytes express proinflammatory cytokines and chemokines capable of modulating a local inflammatory cascade in articular cartilage, which could potentially lead to focal degradation and osteoarthritis.
Publication Date: 2007-04-28 PubMed ID: 17461946DOI: 10.1111/j.1532-950X.2007.00253.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigated how the expression of certain inflammatory markers in horse cartilage cells might be influenced by a specific stimulus, interleukin-1beta (IL-1beta). The results highlighted potential pathways for the development of osteoarthritis in horses.
Research Methods
- The study set out to evaluate the gene expression of pro and anti-inflammatory cytokines and chemokines in horse cartilage cells. These cells were subjected to either a control condition (unstimulated) or a test condition in which they were exposed to IL-1beta.
- The cartilage cells for the experiment were obtained from the metacarpophalangeal joints of five different horses, providing ten fetlock samples in total.
- The researchers cultivated chondrocyte monolayer cultures from the cartilage samples. These cultures were subsequently stimulated with a dose of 5 ng/mL of recombinant human IL-1beta.
- After 24 hours, RNA was extracted from the cells for further analysis. Gene expression of IL-1beta, IL-4, IL-6, IL-8, TNF-alpha, and ubiquitin was assessed using the molecular biology technique of real-time RT-PCR.
Research Findings
- In unstimulated cartilage cells, mRNA of certain proinflammatory markers (IL-1beta, IL-6, IL-8) was found. The expression of these markers increased significantly when the cells were stimulated with IL-1beta.
- IL-4 mRNA, however, was not detectable in any of the samples, whether stimulated or unstimulated.
- There was variation in the expression of TNF-alpha mRNA across samples: it was found in two unstimulated samples, and in all samples that had been stimulated with IL-1beta.
Conclusions
- The research demonstrated that equine cartilage cells express mRNA for several proinflammatory cytokines and chemokines, and that their expression can be modulated by IL-1beta.
- These findings suggest that chondrocytes can produce and express proinflammatory cytokines and chemokines, which may incite a local inflammatory response in articular cartilage. This in turn could cause targeted cartilage degradation and potentially lead to conditions such as osteoarthritis.
Cite This Article
APA
David F, Farley J, Huang H, Lavoie JP, Laverty S.
(2007).
Cytokine and chemokine gene expression of IL-1beta stimulated equine articular chondrocytes.
Vet Surg, 36(3), 221-227.
https://doi.org/10.1111/j.1532-950X.2007.00253.x Publication
Researcher Affiliations
- Département de Sciences Cliniques, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada J2S 7C6.
MeSH Terms
- Animals
- Cartilage, Articular / cytology
- Chondrocytes / drug effects
- Chondrocytes / immunology
- Cytokines / biosynthesis
- Cytokines / genetics
- DNA Primers
- Gene Expression Regulation
- Horses / immunology
- Interleukin-1beta / pharmacology
- Interleukin-6 / biosynthesis
- Interleukin-6 / genetics
- Interleukin-8 / biosynthesis
- Interleukin-8 / genetics
- RNA, Messenger / analysis
- Reverse Transcriptase Polymerase Chain Reaction
- Tumor Necrosis Factor-alpha / biosynthesis
- Tumor Necrosis Factor-alpha / genetics
Citations
This article has been cited 19 times.- Costa V, De Fine M, Raimondi L, Bellavia D, Cordaro A, Carina V, Alessandro R, Pignatti G, Fini M, Giavaresi G, De Luca A. Timing Expression of miR203a-3p during OA Disease: Preliminary In Vitro Evidence. Int J Mol Sci 2023 Feb 21;24(5).
- Arévalo-Turrubiarte M, Baratta M, Ponti G, Chiaradia E, Martignani E. Extracellular vesicles from equine mesenchymal stem cells decrease inflammation markers in chondrocytes in vitro. Equine Vet J 2022 Nov;54(6):1133-1143.
- Megha KB, Joseph X, Akhil V, Mohanan PV. Cascade of immune mechanism and consequences of inflammatory disorders. Phytomedicine 2021 Oct;91:153712.
- Velloso Alvarez A, Boone LH, Pondugula SR, Caldwell F, Wooldridge AA. Effects of Autologous Conditioned Serum, Autologous Protein Solution, and Triamcinolone on Inflammatory and Catabolic Gene Expression in Equine Cartilage and Synovial Explants Treated With IL-1β in Co-culture. Front Vet Sci 2020;7:323.
- Mendoza Garcia FJ, Gonzalez-De Cara C, Aguilera-Aguilera R, Buzon-Cuevas A, Perez-Ecija A. Meloxicam ameliorates the systemic inflammatory response syndrome associated with experimentally induced endotoxemia in adult donkeys. J Vet Intern Med 2020 Jul;34(4):1631-1641.
- Guo Y, Tian L, Du X, Deng Z. MiR-203 regulates estrogen receptor α and cartilage degradation in IL-1β-stimulated chondrocytes. J Bone Miner Metab 2020 May;38(3):346-356.
- Nam B, Kim SA, Nam W, Jeung WH, Park SD, Lee JL, Sim JH, Jang SS. Lactobacillus plantarum HY7714 Restores TNF-α Induced Defects on Tight Junctions. Prev Nutr Food Sci 2019 Mar;24(1):64-69.
- Barrachina L, Remacha AR, Romero A, Vitoria A, Albareda J, Prades M, Roca M, Zaragoza P, Vázquez FJ, Rodellar C. Assessment of effectiveness and safety of repeat administration of proinflammatory primed allogeneic mesenchymal stem cells in an equine model of chemically induced osteoarthritis. BMC Vet Res 2018 Aug 17;14(1):241.
- Mata M, Milian L, Oliver M, Zurriaga J, Sancho-Tello M, de Llano JJM, Carda C. In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study. Stem Cells Int 2017;2017:8309256.
- Ríos DL, López C, Carmona JU. Platelet-Rich Gel Supernatants Stimulate the Release of Anti-Inflammatory Proteins on Culture Media of Normal Equine Synovial Membrane Explants. Vet Med Int 2015;2015:547052.
- Shao Y, Cheng Z, Li X, Chernaya V, Wang H, Yang XF. Immunosuppressive/anti-inflammatory cytokines directly and indirectly inhibit endothelial dysfunction--a novel mechanism for maintaining vascular function. J Hematol Oncol 2014 Oct 31;7:80.
- Dušanić D, Benčina D, Narat M, Oven I. Phenotypic characterization of Mycoplasma synoviae induced changes in the metabolic and sensitivity profile of in vitro infected chicken chondrocytes. Biomed Res Int 2014;2014:613730.
- Ownby SL, Fortuno LV, Au AY, Grzanna MW, Rashmir-Raven AM, Frondoza CG. Expression of pro-inflammatory mediators is inhibited by an avocado/soybean unsaponifiables and epigallocatechin gallate combination. J Inflamm (Lond) 2014 Mar 28;11(1):8.
- Zhou X, Wang Z, Chen J, Wang W, Song D, Li S, Yang H, Xue S, Chen C. Increased levels of IL-6, IL-1β, and TNF-α in Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. Rheumatol Int 2014 Jul;34(7):995-1004.
- Hew CS, Khoo BY, Gam LH. The anti-cancer property of proteins extracted from Gynura procumbens (Lour.) Merr. PLoS One 2013;8(7):e68524.
- Nakashima K, Nakatsuka K, Yamashita K, Kenichi K, Taro H. An in vitro model of cartilage degradation by chondrocytes in a three-dimensional culture system. Int J Biomed Sci 2012 Dec;8(4):249-57.
- Khoo BY, Miswan N, Balaram P, Nadarajan K, Elstner E. Modification of MCF-10A cells with pioglitazone and serum-rich growth medium increases soluble factors in the conditioned medium, likely reducing BT-474 cell growth. Int J Mol Sci 2012;13(5):5607-5627.
- Röhner E, Matziolis G, Perka C, Füchtmeier B, Gaber T, Burmester GR, Buttgereit F, Hoff P. Inflammatory synovial fluid microenvironment drives primary human chondrocytes to actively take part in inflammatory joint diseases. Immunol Res 2012 Jun;52(3):169-75.
- El Ezaby NM, Saad EA, El Basuni MA. Acetylsalicylic Acid with Ascorbate: A Promising Combination Therapy for Solid Tumors. Drugs R D 2024 Jun;24(2):303-316.
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