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Veterinary immunology and immunopathology2005; 104(1-2); 91-97; doi: 10.1016/j.vetimm.2004.10.002

Cytokine profiles of peripheral blood and airway CD4 and CD8 T lymphocytes in horses with recurrent airway obstruction.

Abstract: Equine recurrent airway obstruction (RAO) is thought to result from an aberrant immune response to inhaled antigens, modulated by T lymphocytes via the secretion of pro-inflammatory cytokines. However data relating to the phenotypes of the T lymphocytes present in peripheral blood and bronchoalveolar lavage fluid of RAO horses and their cytokine profiles are contradictory. The aim of this study was to further investigate the cytokine (IL-4, IL-5, IL-13 and INF-gamma) mRNA expression profile in peripheral blood lymphocytes and bronchoalveolar lavage lymphocytes from RAO and control horses, before and at 48 h after horses were exposed to hay/straw. In contrast to previous studies, cytokine expression was quantified in populations of CD4 and CD8 T lymphocytes which were purified using magnetic bead antibody cell separation. Hay/straw exposure induced clinical airway obstruction, airway neutrophilia and airway lymphocytosis in RAO horses, and, induced a mild, but significant, airway neutrophilia in controls. However, hay/straw exposure had no significant effect on peripheral blood lymphocyte or bronchoalveolar lavage lymphocyte cytokine expression in either group. In conclusion, RAO was not associated with alterations in lymphocyte cytokine expression that are consistent with Th1 or Th2 responses, but rather with a general down-regulation in expression of the measured cytokines in peripheral blood lymphocytes and bronchoalveolar lavage lymphocytes.
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Publication Date: 2005-01-22 PubMed ID: 15661334DOI: 10.1016/j.vetimm.2004.10.002Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the response of immune cells in horses with recurrent airway obstruction (RAO) when exposed to hay/straw, focusing especially on the expression levels of certain cytokines, proteins involved in immune response. The findings suggest that exposure to hay/straw does not cause significant changes in cytokine expression in peripheral blood lymphocytes or bronchoalveolar lavage lymphocytes, contradicting previous assumptions about RAO.

Objective of the Research

  • The key goal of the study was to explore the expression of certain cytokines (specifically IL-4, IL-5, IL-13, and INF-gamma) in CD4 and CD8 T lymphocytes from peripheral blood and bronchoalveolar lavage fluid in horses with recurrent airway obstruction (RAO) before and after exposure to hay/straw.

Methodology

  • The expression of these cytokines was measured using a technique called magnetic bead antibody cell separation. This method helped to separate and isolate the specific types of T lymphocytes under investigation.
  • The horse subjects were exposed to hay/straw, and the impact on cellular responses and cytokine expressions was measured.

Key Findings

  • Exposure to hay/straw resulted in clinical airway obstruction, an increase in neutrophils and lymphocytes in the airway in RAO horses, which are signs of induced inflammation.
  • In contrast, control (healthy) horses experienced a minor but statistically significant increase in airway neutrophils after hay/straw exposure.
  • However, the same exposure didn’t team up with any significant change in cytokine expression in either peripheral blood or bronchoalveolar lavage lymphocytes in both groups of horses (RAO and control).

Conclusion

  • The study concluded that unlike what was earlier assumed about RAO, the disease does not cause changes in cytokine expression linked to typical Th1 or Th2 responses.
  • Instead, there was a general reduction in the production of the evaluated cytokines in both peripheral blood and bronchoalveolar lavage lymphocytes, implying the immune response in RAO may not directly involve changes in these specific cytokines.

Cite This Article

APA
Kleiber C, McGorum BC, Horohov DW, Pirie RS, Zurbriggen A, Straub R. (2005). Cytokine profiles of peripheral blood and airway CD4 and CD8 T lymphocytes in horses with recurrent airway obstruction. Vet Immunol Immunopathol, 104(1-2), 91-97. https://doi.org/10.1016/j.vetimm.2004.10.002

Publication

Veterinary immunology and immunopathology
ISSN: 0165-2427
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 104
Issue: 1-2
Pages: 91-97

Researcher Affiliations

Kleiber, C
  • Department of Clinical Veterinary Medicine, University of Berne, Länggasstrasse 124, 3012 Berne, Switzerland. claudia.kleiber@knp.unibe.ch
McGorum, B C
    Horohov, D W
      Pirie, R S
        Zurbriggen, A
          Straub, R

            MeSH Terms

            • Airway Obstruction / blood
            • Airway Obstruction / immunology
            • Airway Obstruction / veterinary
            • Animals
            • Bronchoalveolar Lavage Fluid / immunology
            • CD4-Positive T-Lymphocytes / immunology
            • CD8-Positive T-Lymphocytes / immunology
            • Cytokines / genetics
            • Cytokines / immunology
            • Female
            • Horse Diseases / immunology
            • Horses
            • Immunomagnetic Separation / veterinary
            • Male
            • Poaceae / immunology
            • RNA / chemistry
            • RNA / genetics
            • Reverse Transcriptase Polymerase Chain Reaction / veterinary
            • Statistics, Nonparametric

            Citations

            This article has been cited 14 times.
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