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Home / Equine Research Bank / Data Brief / Dataset of the sperm proteome of stallions with different mo...
Data in brief2022; 45; 108578; doi: 10.1016/j.dib.2022.108578

Dataset of the sperm proteome of stallions with different motility.

Abstract: This paper provides a detailed set of data on how the stallion sperm proteome differs among stallions with different sperm motilities, although within normal ranges. Findings distinguish proteins that may help to identify stallions of superior sperm motility. Sperm proteins were analyzed using a UHPLC/MS/MS system comprising of an Agilent 1290 infinity series UHPLC coupled to an Agilent 6550 Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). These data can be used to disclose potential targets to identify good sperm samples and to study specific pathways involved in the regulation of sperm motility. This data article is linked to the paper "Proteins involved in mitochondrial metabolic functions and fertilization predominate in stallions with better motility Journal of Proteomics 247:104335 doi: 10.1016/j.jprot.2021.104335".
© 2022 The Author(s). Published by Elsevier Inc.
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Publication Date: 2022-09-05 PubMed ID: 36131951PubMed Central: PMC9483576DOI: 10.1016/j.dib.2022.108578Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents a comprehensive data set on the differences in the protein makeup (proteome) of stallion sperm, focusing on how these differences relate to sperm motility. The proteins identified could function as markers for superior sperm motility in stallions.

Methodology

  • Scientists examined the sperm proteome using a UHPLC/MS/MS system, which is an advanced analytical instrumentation method that can detect, measure, and analyze different proteins.
  • The biochemical technique employed was made up of an Agilent 1290 Inifinity series UHPLC (Ultra-High Performance Liquid Chromatography) linked to an Agilent 6550 Q-TOF (Quadruple Time of Flight) mass spectrometer. This complex set-up enabled the effective analysis of the protein composition in stallion sperm.

Findings

  • The research identified a unique set of proteins present in sperm samples of stallions exhibiting varying levels of sperm motility.
  • Despite all specimens being within the standard motility ranges, differences in the proteome could suggest why certain stallions have superior sperm motility.
  • The observed proteins could serve as potential indicators for identifying high-quality sperm samples, offering opportunities to optimize breeding strategies.

Application and Future Research

  • The results can be used to investigate specific biochemical pathways involved in the regulation of sperm motility, providing critical insights into male reproductive biology.
  • The study also refers to a related publication: “Proteins involved in mitochondrial metabolic functions and fertilization predominate in stallions with better motility.” This suggests the relevant proteins identified do not only affect sperm motility but could also be involved in energy production (mitochondrial metabolic functions) and fertilization processes.

Cite This Article

APA
Gaitskell-Phillips G, Martín-Cano FE, Ortiz-Rodríguez JM, Silva-Rodríguez A, da Silva-Álvarez E, Gil MC, Ortega-Ferrusola C, Peña FJ. (2022). Dataset of the sperm proteome of stallions with different motility. Data Brief, 45, 108578. https://doi.org/10.1016/j.dib.2022.108578

Publication

Data in brief
ISSN: 2352-3409
NlmUniqueID: 101654995
Country: Netherlands
Language: English
Volume: 45
Pages: 108578
PII: 108578

Researcher Affiliations

Gaitskell-Phillips, Gemma
  • Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
Martín-Cano, Francisco E
  • Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
Ortiz-Rodríguez, José M
  • Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
Silva-Rodríguez, Antonio
  • SIPA University of Extremadura, Cáceres, Spain.
da Silva-Álvarez, Eva
  • Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
Gil, Maria C
  • Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
Ortega-Ferrusola, Cristina
  • Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
Peña, Fernando J
  • Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.

Conflict of Interest Statement

The authors declare that they have no known competing financial interests or personal relationships which have, or could be perceived to have, influenced the work reported in this article.

References

This article includes 9 references
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