Demonstration of three DRB loci in a domestic horse family.
Abstract: Single-strand conformational polymorphism (SSCP) gel electrophoresis and DNA sequencing were used to characterize the second exon of the horse DRB homologue as well as to identify eight new DRB alleles. The SSCP gels presented a complex pattern, with phenotypes exhibiting between 4 and 13 bands. The DRB SSCP patterns were studied for two families (6 to 13 bands per pattern). For both families, the patterns showed simple Mendelian inheritance. The polymerase chain reaction products from two individuals possessing homozygous major histocompatibility complex (MHC) alleles by descent were cloned and retested on SSCP gels. All bands derived from the genomic DNA amplification could be accounted for with bands derived from the cloned DNA amplification products. The results were consistent with three DRB loci, though this number may be variable within the domestic horse population. Gene sequences were variable among the different products, and we were unable to assign locus designations for particular sequences. Amplification of cDNA library material derived from one of the individuals who is MHC homozygous by descent showed an SSCP profile suggesting that all three DRB loci are transcribed into mRNA.
Publication Date: 1996-01-01 PubMed ID: 8824155DOI: 10.1007/BF02602805Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article discusses the identification of three DRB loci in the domestic horse family through single-strand conformational polymorphism and DNA sequencing.
Research Methodology
- The researchers employed single-strand conformational polymorphism (SSCP) gel electrophoresis and DNA sequencing to study the second exon of the DRB homologue in horses.
- SSCP gels produced a diverse pattern with phenotypes exhibiting between 4 and 13 bands. These patterns were examined for two families with 6 to 13 bands per pattern, revealing simple Mendelian inheritance.
- Polymerase chain reaction (PCR) was used to clone products from two individual horses that had homozygous major histocompatibility complex (MHC) alleles by descent. The cloned DNA products were retested on the SSCP gels to verify the results.
Key Findings
- All bands collected from the genomic DNA amplification process were able to be matched with bands derived from the cloned DNA amplification products.
- The results suggested the presence of three DRB loci, indicating a possibility of variation within the domestic horse population.
- The researchers however encountered variations in gene sequences among the different products, and could not assign locus designations for particular sequences.
- Further amplification of cDNA library material from one of the horses who was MHC homozygous by descent suggested that all three DRB loci are transcribed into mRNA.
Conclusions
- The study successfully identified eight new DRB alleles in the horse DRB homologue, demonstrating the presence of three DRB loci.
- These findings shed new light on the genetic composition of the domestic horse population, opening avenues for further investigation into genetic variability within the species.
Cite This Article
APA
Fraser DG, Bailey E.
(1996).
Demonstration of three DRB loci in a domestic horse family.
Immunogenetics, 44(6), 441-445.
https://doi.org/10.1007/BF02602805 Publication
Researcher Affiliations
- Department of Veterinary Science, 108 Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546-0099, USA.
MeSH Terms
- Alleles
- Animals
- Base Sequence
- DNA
- HLA-DR Antigens / genetics
- Horses / genetics
- Horses / immunology
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Single-Stranded Conformational
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Citations
This article has been cited 11 times.- Vasoya D, Tzelos T, Benedictus L, Karagianni AE, Pirie S, Marr C, Oddsdóttir C, Fintl C, Connelley T. High-Resolution Genotyping of Expressed Equine MHC Reveals a Highly Complex MHC Structure.. Genes (Basel) 2023 Jul 10;14(7).
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