Detection of antibodies against Borna disease virus in sera and cerebrospinal fluid of horses in the USA.
Abstract: Sera from 295 horses in the USA were examined by an indirect immunofluorescence assay and Western blot assays to determine the prevalence of Borna disease virus infection. Eight (2.7 per cent) of the samples were positive in both assays, and 18 (6.1 per cent) were positive only in the Western blot assay. The indirect fluorescence titres ranged from 1:20 to 1:80 of antibodies recognising the virus-specific antigen from Borna disease virus-infected cells. The purified virus-specific proteins isolated from infected rat brains were recognised by positive equine serum samples after immunostaining by a Western blot technique. Information obtained from the owners about the history of the seropositive horses revealed that they were either clinically normal or had a pathological diagnosis of disease unrelated to Borna disease. This is the first report of the detection of antibodies to Borna disease virus in horses in the USA. The disease may be more widespread in a subclinical form, with very long incubation periods, and may not necessarily be restricted to historically endemic areas.
Publication Date: 1993-03-06 PubMed ID: 8460459DOI: 10.1136/vr.132.10.241Google Scholar: Lookup
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- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The study investigates the presence of Borna disease virus infection in horses in the USA, revealing that the disease may be more widespread than previously known, and may not be confined to historically endemic areas.
Research Methodology
- The researchers collected sera from 295 horses in the USA to conduct their study.
- An indirect immunofluorescence assay and Western blot assays were used to test these samples.
- The intent was to find out the prevalence of Borna disease virus infection in these horses.
Findings
- Of all the samples examined, 2.7% were positive for the disease in both the indirect immunofluorescence assay and the Western blot assay.
- An additional 6.1% of the samples were found positive only in the Western blot assay.
- The indirect fluorescence titres ranged from 1:20 to 1:80 of antibodies, recognising the virus-specific antigen from Borna disease virus-infected cells.
- The virus-specific proteins, isolated from infected rat brains, were recognised by positive equine serum samples through a Western blot technique after immunostaining.
Further Information and Conclusions
- The researchers obtained additional information from the owners of the horses, revealing that the seropositive horses were either clinically normal or had a disease diagnosis unrelated to Borna disease.
- The study marks the first report of detection of antibodies to Borna disease virus in horses in the USA, which indicates that the disease could be more widespread in a subclinical form and not necessarily restricted to historically endemic areas.
- A crucial aspect indicated by the researchers is the disease’s very long incubation periods, allowing it to potentially spread undetected.
Cite This Article
APA
Kao M, Hamir AN, Rupprecht CE, Fu ZF, Shankar V, Koprowski H, Dietzschold B.
(1993).
Detection of antibodies against Borna disease virus in sera and cerebrospinal fluid of horses in the USA.
Vet Rec, 132(10), 241-244.
https://doi.org/10.1136/vr.132.10.241 Publication
Researcher Affiliations
- Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104-4268.
MeSH Terms
- Animals
- Antibodies, Viral / blood
- Antibodies, Viral / cerebrospinal fluid
- Blotting, Western
- Borna Disease / epidemiology
- Borna disease virus / immunology
- Breeding
- Cell Line
- Cricetinae
- Female
- Fluorescent Antibody Technique
- Horse Diseases / epidemiology
- Horses
- Male
- Prevalence
- Rabbits
- Rats
- United States / epidemiology
Grant Funding
- AI-09701 / NIAID NIH HHS
- MH-45174 / NIMH NIH HHS
Citations
This article has been cited 22 times.- Anderson C, Baha H, Boghdeh N, Barrera M, Alem F, Narayanan A. Interactions of Equine Viruses with the Host Kinase Machinery and Implications for One Health and Human Disease. Viruses 2023 May 13;15(5).
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