Determination of the activity of diclazuril against Sarcocystis neurona and Sarcocystis falcatula in cell cultures.
Abstract: Diclazuril is a benzeneacetonitril anticoccidial that has excellent activity against the extraintestinal stages of Toxoplasma gondii and Neospora caninum. It also is highly active against intestinal coccidia of poultry. The present study examined the efficacy of diclazuril in inhibiting merozoite production of Sarcocystis neurona and Sarcocystis falcatula in bovine turbinate cell cultures. Diclazuril inhibited merozoite production by more than 80% in cultures of S. neurona or S. falcatula treated with 0.1 ng/ml diclazuril and greater than 95% inhibition of merozoite production was observed when infected cultures were treated with 1.0 ng/ml diclazuril. Diclazuril may have promise as a therapeutic agent in the treatment of S. neurona-induced equine protozoal myeloencephalitis in horses and S. falcatula infections in birds.
Publication Date: 2000-03-04 PubMed ID: 10701584DOI: 10.1645/0022-3395(2000)086[0164:DOTAOD]2.0.CO;2Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article examines the efficacy of the drug diclazuril against two types of parasites, Sarcocystis neurona and Sarcocystis falcatula, in lab-grown cell cultures. The result showed that diclazuril successfully inhibited the growth of these parasites, suggesting potential therapeutic usage in horses and birds affected by these parasites.
Understanding the Context
- The key focus of this study is diclazuril, a type of anticoccidial drug that’s shown effectiveness against species like Toxoplasma gondii and Neospora caninum. Anticoccidials are medications used for treating coccidiosis, a parasitic disease in animals.
- The parasites in question are Sarcocystis neurona and Sarcocystis falcatula. The former is known to induce equine protozoal myeloencephalitis, a serious disease in horses affecting the central nervous system, while the latter is notable for infections in birds.
Methodology of the Study
- The researchers tested the efficacy of diclazuril on bovine turbinate cell cultures infected with either S. neurona or S. falcatula. Bovine turbinate cell cultures are a common substrate used for such studies due to their ability to support the growth of a wide range of viruses.
Results of the Study
- The study reports that diclazuril was successful in inhibiting the growth of merozoites, the asexual reproductive form of these parasites. There was more than 80% reduction in merozoite production in cultures treated with 0.1 ng/ml of diclazuril.
- The efficacy of diclazuril increased with its dosage: over 95% inhibition of merozoite production was noted in cultures treated with 1.0 ng/ml of diclazuril.
Implications of the Study
- Based on these results, the researchers suggest that diclazuril might be considered as a potential therapeutic agent in treating infections caused by these types of Sarcocystis in horses and birds.
- This is significant as it indicates a possible new treatment method for these parasitic infections that can cause serious health issues in animals.
Cite This Article
APA
Lindsay DS, Dubey JP.
(2000).
Determination of the activity of diclazuril against Sarcocystis neurona and Sarcocystis falcatula in cell cultures.
J Parasitol, 86(1), 164-166.
https://doi.org/10.1645/0022-3395(2000)086[0164:DOTAOD]2.0.CO;2 Publication
Researcher Affiliations
- Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061-0342, USA.
MeSH Terms
- Animals
- Cattle
- Cell Line
- Coccidiostats / pharmacology
- Horses
- Nitriles / pharmacology
- Opossums
- Parrots
- Sarcocystis / drug effects
- Triazines / pharmacology
Citations
This article has been cited 4 times.- Bowden GD, Land KM, O'Connor RM, Fritz HM. High-throughput screen of drug repurposing library identifies inhibitors of Sarcocystis neurona growth. Int J Parasitol Drugs Drug Resist 2018 Apr;8(1):137-144.
- Reed SM, Furr M, Howe DK, Johnson AL, MacKay RJ, Morrow JK, Pusterla N, Witonsky S. Equine Protozoal Myeloencephalitis: An Updated Consensus Statement with a Focus on Parasite Biology, Diagnosis, Treatment, and Prevention. J Vet Intern Med 2016 Mar-Apr;30(2):491-502.
- Dubey JP, Howe DK, Furr M, Saville WJ, Marsh AE, Reed SM, Grigg ME. An update on Sarcocystis neurona infections in animals and equine protozoal myeloencephalitis (EPM). Vet Parasitol 2015 Apr 15;209(1-2):1-42.
- Oz HS, Tobin T. Diclazuril Protects against Maternal Gastrointestinal Syndrome and Congenital Toxoplasmosis. Int J Clin Med 2014 Jan 1;5(3):93-101.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
