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Determination of the correlation between stallion’s age and number of sex chromosome aberrations in spermatozoa.
Abstract: The aim of this study was a cytogenetic analysis of stallions semen to find sex chromosome aberrations and to determine if there was an association between stallion's age and aberration frequency for the sex chromosomes. Sperm samples were collected from 22 stallions of various age from 3 to 23 years. Multicolour FISH was performed on each sample, using probes for the sex chromosomes and EGFR gene, localized on 4p12 in domestic horse. A total of 26199 sperm cells were analysed (from 1 070 to 1 532 per animal). Among the analysed cells, there were 50.318% with X chromosome, 48.543% with Y chromosome and 1.139% with aberrant chromosomes. The frequency of aberrations was: sex chromosomes nullisomy (0.466%), XY aneuploidy (0.454%), XX disomy (0.146%), YY disomy (0.041%), diploidy (0.024%) and trisomy XXY (0.008%). Additionally there was a correlation between the age of an animal and the frequency of sex chromosome aberration and a significant positive correlation between age and disomy of XY, XX, YY, trisomy of XXY, autosomal disomy was seen. A Correlation between the age of a stallion and the level of nullisomy was negative. The present study demonstrated that FISH technique is a powerful method to identify sex chromosome aberrations in equine spermatozoa and might be very helpful for a breeder during a selection for the best stallion.
© 2011 Blackwell Verlag GmbH.
Publication Date: 2011-02-16 PubMed ID: 21323752DOI: 10.1111/j.1439-0531.2010.01742.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study investigates the link between a stallion’s age and the number of sex chromosome aberrations in their sperm. Utilizing a cytogenetic analysis of semen from 22 stallions across a wide age range, the study found a correlation between the frequency of such aberrations and the horses’ age.
Sampling and Testing Process
- The study involved collecting semen samples from 22 different stallions, aged between 3 and 23 years old.
- Each sample underwent a Multicolour FISH (Fluorescence in situ hybridization) test, a technique used to visualize and map the genetic material in the cells. In this case, the researchers used probes for the sex chromosomes and the EGFR gene found in domestic horses.
- Overall, the researchers analyzed a total of 26,199 sperm cells, varying from 1,070 to 1,532 per stallion.
Findings
- The analysis revealed that, of the analyzed cells, roughly 50.318% contained an X chromosome, 48.543% had a Y chromosome, and the remaining 1.139% had aberrant chromosomes.
- Several types of aberrations were found, including nullisomy (missing both copies of a chromosome pair), XY aneuploidy (an abnormal number of chromosomes), disomy (having two copies of a chromosome from one parent and none from the other), diploidy (having two complete sets of chromosomes), and trisomy XXY (an extra copy of the X chromosome).
- The study found a correlation between the frequency of these aberrations and the age of the stallions. As such, the older the animal, the greater the frequency of XY, XX, YY disomy, XXY trisomy, and autosomal disomy (two non-sex chromosomes from one parent). However, there was a negative correlation with the age and level of nullisomy.
Implications
- The results indicate that the FISH technique is a powerful tool for identifying sex chromosome aberrations in equine spermatozoa.
- This information can be very beneficial for breeders while selecting the best stallion, as older animals might have a higher risk of producing offsprings with sex chromosome aberrations.
Cite This Article
APA
Bugno-Poniewierska M, Kozub D, Pawlina K, Tischner M, Tischner M, Słota E, Wnuk M.
(2011).
Determination of the correlation between stallion’s age and number of sex chromosome aberrations in spermatozoa.
Reprod Domest Anim, 46(5), 787-792.
https://doi.org/10.1111/j.1439-0531.2010.01742.x Publication
Researcher Affiliations
- Department of Animal Immuno- and Cytogenetics, National Research Institute of Animal Production, Balice, Poland. mbugno@izoo.krakow.pl
MeSH Terms
- Aging / physiology
- Animals
- Horses / physiology
- Male
- Semen Analysis / veterinary
- Sex Chromosome Aberrations / veterinary
Citations
This article has been cited 3 times.- Guyot C, Gandula M, Noordermeer W, François-Brazier C, Moigno R, Bessonnat J, Brouillet S, Dhellemmes M, Bidart M, Arnoult C, Satre V, Coutton C, Martinez G. FISH and Chimps: Insights into Frequency and Distribution of Sperm Aneuploidy in Chimpanzees (Pan troglodytes). Int J Mol Sci 2021 Sep 27;22(19).
- Bugno-Poniewierska M, Raudsepp T. Horse Clinical Cytogenetics: Recurrent Themes and Novel Findings. Animals (Basel) 2021 Mar 16;11(3).
- Shilton CA, Kahler A, Davis BW, Crabtree JR, Crowhurst J, McGladdery AJ, Wathes DC, Raudsepp T, de Mestre AM. Whole genome analysis reveals aneuploidies in early pregnancy loss in the horse. Sci Rep 2020 Aug 7;10(1):13314.
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