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Stem cell research & therapy2025; 16(1); 162; doi: 10.1186/s13287-025-04287-5

Determination of the miRNA profile of extracellular vesicles from equine mesenchymal stem cells after different treatments.

Abstract: Osteoarthritis (OA) is a common and incurable disease in humans and animals. To gain a better understanding of the pathogenesis and identify potential treatments, miRNAs will be extracted and analysed from extracellular vesicles (EVs) of equine adipose derived mesenchymal stem cells (AdMSCs). Methods: For this purpose we cultivated and pretreated AdMSCs under different conditions: interleukin 1β, shock wave, chondrogenic differentiation, chondrogenic differentiation under hypoxia, or after senescence. After treatment, EVs were harvested from the cell culture supernatants. Next-generation sequencing (NGS) was used to sequence the miRNAs from the EVs. Results: A total of 89 miRNAs whose expression was significantly altered compared with that of an untreated negative control were identified. On average, 53 miRNAs were upregulated and 6 miRNAs were downregulated. Among others, the miRNAs eca-miR-101, eca-miR-143, eca-miR-145, eca-miR-146a, eca-miR-27a, eca-miR-29b, eca-miR-93, eca-miR-98, and eca-miR-221 were significantly increased after the stimulations, which, as known anti-inflammatory miRNAs, could be candidates for therapeutic use in the treatment of OA. Conclusions: These results lay the foundation for further research into the significance and efficacy of these miRNAs so that this knowledge can be improved in further experiments and, ideally, translated into therapeutic use.
© 2025. The Author(s).
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Publication Date: 2025-04-05 PubMed ID: 40188160PubMed Central: PMC11972531DOI: 10.1186/s13287-025-04287-5Google Scholar: Lookup
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APA
Klymiuk MC, Speer J, Marco I, Elashry MI, Heimann M, Wenisch S, Arnhold S. (2025). Determination of the miRNA profile of extracellular vesicles from equine mesenchymal stem cells after different treatments. Stem Cell Res Ther, 16(1), 162. https://doi.org/10.1186/s13287-025-04287-5

Publication

Stem cell research & therapy
ISSN: 1757-6512
NlmUniqueID: 101527581
Country: England
Language: English
Volume: 16
Issue: 1
Pages: 162
PII: 162

Researcher Affiliations

Klymiuk, Michele C
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany. michele.klymiuk@uni-giessen.de.
Speer, Julia
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany.
Marco, Isabelle De
  • Clinic of Small Animals, c/o Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany.
Elashry, Mohamed I
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany.
Heimann, Manuela
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany.
Wenisch, Sabine
  • Clinic of Small Animals, c/o Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany.
Arnhold, Stefan
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany.

MeSH Terms

  • Animals
  • Mesenchymal Stem Cells / metabolism
  • Mesenchymal Stem Cells / cytology
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • Horses
  • Extracellular Vesicles / metabolism
  • Extracellular Vesicles / genetics
  • Cell Differentiation
  • Osteoarthritis / genetics
  • Chondrogenesis / genetics
  • Cells, Cultured

Conflict of Interest Statement

Declarations. Ethics approval and consent to participate: The authorization for the tissue collection was obtained at the 16th of May 2018 from the local authorities (Regierungspräsidium Gießen, Wetzlar, Germany) and is registered under the number V 54 − 19 c 20 15 h 02 GI 18/1 kTV 1/2018. There is no special project name for this permission. Consent for publication: Not applicable. Artificial intelligence (AI): The authors declare that they have not use AI-generated work in this manuscript. Competing interests: Not applicable.

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