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Development and laboratory evaluation of two lateral flow devices for the detection of vesicular stomatitis virus in clinical samples.
Abstract: Two lateral flow devices (LFD) for the detection of vesicular stomatitis (VS) virus (VSV), types Indiana (VSV-IND) and New Jersey (VSV-NJ) were developed using monoclonal antibodies C1 and F25VSVNJ-45 to the respective VSV serotypes. The performance of the LFDs was evaluated in the laboratory on suspensions of vesicular epithelia and cell culture passage derived supernatants of VSV. The collection of test samples included 105 positive for VSV-IND (92 vesicular epithelial suspensions and 13 cell culture antigens; encompassing 93 samples of subtype 1 [VSV-IND-1], 9 of subtype 2 [VSV-IND-2] and 3 of subtype 3 [VSV-IND-3]) and 189 positive for VSV-NJ (162 vesicular epithelial suspensions and 27 cell culture antigens) from suspected cases of vesicular disease in cattle and horses collected from 11 countries between 1937 and 2008 or else were derived from experimental infection and 777 samples that were either shown to be positive or negative for foot-and-mouth disease (FMD) virus (FMDV) and swine vesicular disease virus (SVDV) or else collected from healthy cattle or pigs and collected from 68 countries between 1965 and 2011. The diagnostic sensitivity of the VSV-IND (for reaction with VSV-IND-1) and VSV-NJ LFDs was either similar or identical at 94.6% (VSV-IND) and 97.4% (VSV-NJ) compared to 92.5% and 97.4% obtained by the reference method of antigen ELISA. The VSV-IND LFD failed to react with viruses of VSV-IND-2 and 3, while the VSV-NJ device recognized all VSV-NJ virus strains. The diagnostic specificities of the VSV-IND and VSV-NJ LFDs were 99.1% and 100, respectively, compared to 99.6% and 99.8% for the ELISA. Reactions with FMDV which can produce indistinguishable syndromes clinically in cattle, pigs and sheep and SVDV (vesicular disease in pigs) did not occur. These data illustrate the potential for the LFDs to be used next to the animal for providing rapid and objective support to veterinarians in their clinical judgment of vesicular disease and for the subtype (VSV-IND-1) and type-specific (VSV-NJ) pen-side diagnosis of VS and differential diagnosis from FMD.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication Date: 2011-12-29 PubMed ID: 22230813DOI: 10.1016/j.jviromet.2011.12.010Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper discusses the development of two lateral flow devices (LFDs) that effectively detect the presence of Vesicular Stomatitis Virus (VSV) in clinical samples from animals, and compares their efficiency to the reference method, antigen ELISA.
Development of Lateral Flow Devices
- The researchers developed two Lateral Flow Devices (LFDs), specifically for the detection of two types of Vesicular Stomatitis Virus (VSV): Indiana (VSV-IND) and New Jersey (VSV-NJ).
- Monoclonal antibodies C1 and F25VSVNJ-45 were used to identify the respective VSV serotypes. These antibodies bind specifically to the target antigens present in the respective VSV strains, allowing for precise detection.
Evaluation of LFDs
- The performance of the LFDs was evaluated using vesicular epithelia suspensions and cell culture-derived supernatants of VSV, acting as test samples to simulate real-world usage.
- The test samples collection included cases positive for both strains of the virus and some samples positive for Foot-and-Mouth disease (FMD) or Swine Vesicular Disease Virus (SVDV).
- The test samples were derived from suspected cases of vesicular disease in cattle and horses collected from 11 countries over several decades (from 1937 to 2008), and also samples from healthy animals or ones affected with FMD or SVD, collected from 68 countries between 1965 and 2011.
Performance of LFDs
- The diagnostic sensitivity of the VSV-IND and VSV-NJ LFDs was found to be similar or identical to that obtained by the standard method, antigen ELISA. This implies that LFDs are capable of correctly identifying positive cases.
- However, the VSV-IND LFD did not react with the VSV-IND-2 and 3 variants, limiting its application. The VSV-NJ device, on the other hand, recognized all VSV-NJ virus strains.
- The diagnostic specificities, or the ability of the tests to correctly identify negative cases, of both the LFDs were also nearly similar to the ELISA with the VSV-IND and VSV-NJ LFDs showing 99.1% and 100% specificity respectively.
Usefulness of LFDs
- The research findings suggest that these LFDs could provide quick and accurate support to veterinarians in their clinical diagnosis of vesicular disease.
- They could facilitate the identification of subtype (VSV-IND-1) and type-specific (VSV-NJ) strains of the virus and differentiate it from other diseases, such as foot-and-mouth disease.
Cite This Article
APA
Ferris NP, Clavijo A, Yang M, Velazquez-Salinas L, Nordengrahn A, Hutchings GH, Kristersson T, Merza M.
(2011).
Development and laboratory evaluation of two lateral flow devices for the detection of vesicular stomatitis virus in clinical samples.
J Virol Methods, 180(1-2), 96-100.
https://doi.org/10.1016/j.jviromet.2011.12.010 Publication
Researcher Affiliations
- Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF, UK. nigel.ferris@iah.ac.uk
MeSH Terms
- Animals
- Cattle
- Cattle Diseases / diagnosis
- Cattle Diseases / virology
- Diagnosis, Differential
- Enterovirus B, Human / isolation & purification
- Enterovirus Infections / diagnosis
- Enterovirus Infections / virology
- Enzyme-Linked Immunosorbent Assay
- Foot-and-Mouth Disease / diagnosis
- Foot-and-Mouth Disease Virus
- Laboratories / supply & distribution
- Sensitivity and Specificity
- Sheep
- Sheep Diseases / diagnosis
- Sheep Diseases / virology
- Swine
- Vesicular Stomatitis / diagnosis
- Vesicular Stomatitis / virology
- Vesicular stomatitis Indiana virus / isolation & purification
- Vesicular stomatitis New Jersey virus / isolation & purification
Grant Funding
- Biotechnology and Biological Sciences Research Council
Citations
This article has been cited 4 times.- Weber M, Gierschner P, Klassen A, Kasbohm E, Schubert JK, Miekisch W, Reinhold P, Köhler H. Detection of Paratuberculosis in Dairy Herds by Analyzing the Scent of Feces, Alveolar Gas, and Stable Air. Molecules 2021 May 11;26(10).
- Xue X, Yu Z, Jin H, Liang L, Li J, Li X, Wang Y, Cui S, Li G. Recombinant adenovirus expressing vesicular stomatitis virus G proteins induce both humoral and cell-mediated immune responses in mice and goats. BMC Vet Res 2021 Jan 18;17(1):36.
- Wong CL, Yong CY, Ong HK, Ho KL, Tan WS. Advances in the Diagnosis of Foot-and-Mouth Disease. Front Vet Sci 2020;7:477.
- Eggerbauer E, de Benedictis P, Hoffmann B, Mettenleiter TC, Schlottau K, Ngoepe EC, Sabeta CT, Freuling CM, Müller T. Evaluation of Six Commercially Available Rapid Immunochromatographic Tests for the Diagnosis of Rabies in Brain Material. PLoS Negl Trop Dis 2016 Jun;10(6):e0004776.
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