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Microbiology (Reading, England)2008; 154(Pt 10); 3016-3024; doi: 10.1099/mic.0.2008/018911-0

Development of an unambiguous and discriminatory multilocus sequence typing scheme for the Streptococcus zooepidemicus group.

Abstract: The zoonotic pathogen Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is commonly found harmlessly colonizing the equine nasopharynx. Occasionally, strains can invade host tissues or cross species barriers, and S. zooepidemicus is associated with numerous different diseases in a variety of hosts, including inflammatory airway disease and abortion in horses, pneumonia in dogs and meningitis in humans. A biovar of S. zooepidemicus, Streptococcus equi subsp. equi, is the causative agent of strangles, one of the most important infections of horses worldwide. We report here the development of the first multilocus sequence typing (MLST) scheme for S. zooepidemicus and its exploitation to define the population genetic structure of these related pathogens. A total of 130 unique sequence types were identified from 277 isolates of diverse geographical and temporal origin. Isolates of S. equi shared a recent evolutionary ancestor with isolates of S. zooepidemicus that were significantly associated with cases of uterine infection or abortion in horses (P<0.001). Isolates of S. zooepidemicus from three UK outbreaks of acute fatal haemorrhagic pneumonia in dogs during 1999, 2001 and 2008 were found to be related to isolates from three outbreaks of this disease in the USA during 2005, 1993 and 2006, respectively. Our data provide strong evidence that S. equi evolved from an ancestral S. zooepidemicus strain and that certain related strains of S. zooepidemicus have a greater propensity to infect particular hosts and tissues.
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Publication Date: 2008-10-04 PubMed ID: 18832307DOI: 10.1099/mic.0.2008/018911-0Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers developed a multilocus sequence typing (MLST) system to study the genetic structure of the zoonotic pathogen Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) and related organisms. This is helpful in understanding the evolution of the pathogens and identifying strains that are more likely to infect specific hosts and tissues.

Understanding the study

  • The study involves Streptococcus equi subsp. zooepidemicus (S. zooepidemicus), a bacteria species that typically lives harmlessly in the nasal passages (nasopharynx) of horses. However, some strains can cause disease in different animals, including horses, dogs, and humans. The diseases can range from inflammation and abortion in horses, pneumonia in dogs, to meningitis in humans. A variant of S. zooepidemicus, Streptococcus equi subsp. equi, is responsible for a major horse disease known as strangles.
  • The researchers developed a way of categorizing and distinguishing the different strains of S. zooepidemicus, using a method known as multilocus sequence typing (MLST). This method involves comparing the DNA sequences at multiple loci (sites) within the genome of the pathogens.
  • A total of 130 unique sequence types were identified, from 277 different S. zooepidemicus samples, which came from diverse geographical and temporal origins.

Key findings

  • The MLST analysis revealed that S. zooepidemicus strains associated with uterine infection or horse abortion shared a recent evolutionary ancestor with S. equi strains. This provides evidence that S. equi evolved from a strain of S. zooepidemicus.
  • The researchers found a link between S. zooepidemicus strains causing fatal pneumonia in dogs during specific outbreaks in the UK (1999, 2001, 2008) and US (1993, 2006, 2005). This suggests that similar strains cause similar disease outbreaks across geographical boundaries.
  • Lastly, the analysis indicates that certain related strains of S. zooepidemicus have a higher likelihood of infecting particular host animals and tissues. This knowledge could be useful in disease prevention and treatment.

Implications of the study

  • Understanding the genetic structure and evolution of S. zooepidemicus and related pathogens could help in identifying the disease-causing strains and in developing suitable treatment strategies.
  • The findings may shed light on the transmission of diseases between different species, contributing to the field of zoonotic disease control.
  • This MLST scheme could potentially be implemented for similar research in other bacterial species, widening its applicability.

Cite This Article

APA
Webb K, Jolley KA, Mitchell Z, Robinson C, Newton JR, Maiden MCJ, Waller A. (2008). Development of an unambiguous and discriminatory multilocus sequence typing scheme for the Streptococcus zooepidemicus group. Microbiology (Reading), 154(Pt 10), 3016-3024. https://doi.org/10.1099/mic.0.2008/018911-0

Publication

Microbiology (Reading, England)
ISSN: 1350-0872
NlmUniqueID: 9430468
Country: England
Language: English
Volume: 154
Issue: Pt 10
Pages: 3016-3024

Researcher Affiliations

Webb, Katy
  • Centre for Preventive Medicine, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK.
Jolley, Keith A
  • The Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3SY, UK.
Mitchell, Zoe
  • Centre for Preventive Medicine, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK.
Robinson, Carl
  • Centre for Preventive Medicine, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK.
Newton, J Richard
  • Centre for Preventive Medicine, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK.
Maiden, Martin C J
  • The Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3SY, UK.
Waller, Andrew
  • Centre for Preventive Medicine, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK.

MeSH Terms

  • Animals
  • Bacterial Typing Techniques / methods
  • Chromosomes, Bacterial / genetics
  • Cluster Analysis
  • DNA, Bacterial / genetics
  • Dog Diseases / microbiology
  • Dogs / microbiology
  • Equidae / microbiology
  • Evolution, Molecular
  • Genetic Variation
  • Genetics, Population
  • Horse Diseases / microbiology
  • Horses / microbiology
  • Polymerase Chain Reaction
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / veterinary
  • Streptococcus equi / classification
  • Streptococcus equi / genetics

Grant Funding

  • 047072 / Wellcome Trust

Citations

This article has been cited 46 times.
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