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Home / Equine Research Bank / Equine Vet J / Differences in plasma and peritoneal fluid proteomes identif...
Equine veterinary journal2019; 51(6); 727-732; doi: 10.1111/evj.13094

Differences in plasma and peritoneal fluid proteomes identifies potential biomarkers associated with survival following strangulating small intestinal disease.

Abstract: Strangulating small intestinal disease (SSID) carries a poor prognosis for survival in comparison to other types of colic, particularly if resection is required. Identification of markers which aid early diagnosis may prevent the need for resection, assist with more accurate prognostication and/or support the decision on whether surgical intervention is likely to be successful, would be of significant welfare benefit. Objective: To apply an unbiased methodology to investigate the plasma and peritoneal fluid proteomes in horses diagnosed with SSID requiring resection, to identify novel biomarkers which may be of diagnostic or prognostic value. Methods: Prospective clinical study. Methods: Plasma and peritoneal fluid from horses presented with acute abdominal signs consistent with SSID was collected at initial clinical examination. Samples from eight horses diagnosed with SSID at surgery in which resection of affected bowel was performed and four control horses subjected to euthanasia for orthopaedic conditions were submitted for liquid chromatography tandem mass spectrometry. Protein expression profiles were determined using label-free quantification. Data were analysed using analysis of variance to identify differentially expressed proteins between control and all SSID horses and SSID horses which survived to hospital discharge and those which did not. Significance was assumed at P≤0.05. Results: A greater number of proteins were identified in peritoneal fluid than plasma of both SSID cases and controls, with 123 peritoneal fluid and 13 plasma proteins significantly differentially expressed (DE) between cases and controls (P<0.05, ≥2 fold change). Twelve peritoneal fluid proteins (P<0.036) and four plasma proteins (P<0.05) were significantly DE between SSID horses which survived and those which did not. Conclusions: A low number of samples were analysed, there was variation in duration and severity of SSID and only short-term outcome was considered. Conclusions: Changes in peritoneal fluid proteome may provide a sensitive indicator of small intestinal strangulation and provide biomarkers relevant to prognosis.
© 2019 EVJ Ltd.
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Publication Date: 2019-03-28 PubMed ID: 30854696DOI: 10.1111/evj.13094Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the difference in plasma and peritoneal fluid proteins in horses diagnosed with strangulating small intestinal disease (SSID) to identify potential biomarkers that may help in early diagnosis and prognosis of the condition.

Research Objective

  • The main objective of this research was to use an unbiased methodology to study the plasma and peritoneal fluid proteomes in horses with SSID that required surgical intervention, in an attempt to identify new biomarkers of potential diagnostic or prognostic value.

Methodology

  • A prospective clinical study was conducted. Plasma and peritoneal fluid were collected from horses presenting with acute abdominal signs consistent with SSID at initial clinical examination.
  • These samples were obtained from eight horses diagnosed with SSID undergoing surgery and four control horses euthanized due to orthopaedic conditions. The samples were subjected to liquid chromatography tandem mass spectrometry to determine protein expression profiles.
  • They used label-free quantification for data analysis and the data was analysed using analysis of variance to identify proteins that were differentially expressed between control and SSID horses and between SSID horses that survived and those that did not. Significance was determined based on a P≤0.05 threshold.

Results

  • The number of proteins identified was greater in peritoneal fluid than in plasma, in both SSID cases and controls. There were 123 peritoneal fluid and 13 plasma proteins significantly differentially expressed between cases and controls.
  • 12 peritoneal fluid proteins and 4 plasma proteins were significantly differentially expressed between SSID horses that survived and those that didn’t. The significant values in both cases were below the P=0.05 threshold.

Conclusions

  • Though only a low number of samples were analysed and there were variations in the duration and severity of SSID among the samples tested, and only short-term outcomes were considered, the study revealed valuable findings.
  • Changes in peritoneal fluid proteome may serve as sensitive indicators of small intestinal strangulation and could provide biomarkers relevant to prognosis.

In the context of the broader significance of this work, the identification of these novel potential biomarkers associated with survival following SSID could aid in early diagnosis and make prognostication more accurate, thus greatly improving animal welfare.

Cite This Article

APA
Bardell D, Milner PI, Goljanek-Whysall K, Peffers MJ. (2019). Differences in plasma and peritoneal fluid proteomes identifies potential biomarkers associated with survival following strangulating small intestinal disease. Equine Vet J, 51(6), 727-732. https://doi.org/10.1111/evj.13094

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 51
Issue: 6
Pages: 727-732

Researcher Affiliations

Bardell, D
  • Institute of Ageing and Chronic Disease, Department of Musculoskeletal Biology, University of Liverpool, Liverpool, UK.
  • Institute of Veterinary Science, University of Liverpool, Neston, Wirral, UK.
Milner, P I
  • Institute of Ageing and Chronic Disease, Department of Musculoskeletal Biology, University of Liverpool, Liverpool, UK.
  • Institute of Veterinary Science, University of Liverpool, Neston, Wirral, UK.
Goljanek-Whysall, K
  • Institute of Ageing and Chronic Disease, Department of Musculoskeletal Biology, University of Liverpool, Liverpool, UK.
Peffers, M J
  • Institute of Ageing and Chronic Disease, Department of Musculoskeletal Biology, University of Liverpool, Liverpool, UK.

MeSH Terms

  • Animals
  • Ascitic Fluid / chemistry
  • Biomarkers / blood
  • Biomarkers / chemistry
  • Female
  • Horse Diseases / blood
  • Horse Diseases / diagnosis
  • Horse Diseases / pathology
  • Horses
  • Intestinal Diseases / veterinary
  • Intestine, Small / pathology
  • Male
  • Prospective Studies
  • Proteome

Grant Funding

  • MR/P020941/1 / Medical Research Council
  • R545/0217 / The Dunhill Medical Trust
  • University of Liverpool Technology Directorate Voucher Scheme
  • University of Liverpool MBI Departmental Research Support Budget

References

This article includes 41 references
  1. Phillips TJ, Walmsley JP. Retrospective analysis of the results of 151 exploratory laparotomies in horses with gastrointestinal disease.. Equine Vet. J. 25, 427-431.
  2. Christophersen MT, Dupont N, Berg-Sørensen KS, Konnerup C, Pihl TH, Andersen PH. Short-term survival and mortality rates in a retrospective study of colic in 1588 Danish horses.. Acta Vet. Scand. 56, 20.
  3. Allen D, White NA, Tyler DE. Factors for prognostic use in equine obstructive small intestinal disease.. J. Am. Vet. Med. Ass. 189, 777-780.
  4. Mair TS, Smith LJ. Survival and complication rates in 300 horses undergoing surgical treatment of colic. Part 1: Short-term survival following a single laparotomy.. Equine Vet. J. 37, 296-302.
  5. Freeman DE, Hammock P, Baker GJ, Goetz T, Foreman JH, Schaeffer DJ, Richter RA, Inoue O, Magid JH. Short and long term survival and prevalence of postoperative ileus after small intestinal surgery in the horse.. Equine Vet. J. 32, Suppl. 32, 42-51.
  6. Proudman CJ, Smith JE, Edwards GB, French NP. Long term survival of equine surgical colic cases. Part 1: patterns of mortality and morbidity.. Equine Vet. J. 34, 432-437.
  7. Morton AJ, Blikslager AT. Surgical and postoperative factors influencing short-term survival of horses following small intestinal resection: 92 cases (1994-2001).. Equine Vet. J. 34, 450-454.
  8. Proudman CJ, Edwards GB, Barnes J, French NP. Factors affecting long-term survival of horses recovering from surgery of the small intestine.. Equine Vet. J. 37, 360-365.
  9. Mair TS, Smith LJ. Survival and complication rates in 300 horses undergoing surgical treatment of colic. Part 3: long-term complications and survival.. Equine Vet. J. 37, 310-314.
  10. Tarn AC, Lapworth R. Biochemical analysis of ascitic (peritoneal) fluid: what should we measure?. Ann. Clin. Biochem. 47, 397-407.
  11. Zhu W, Smith JW, Huang C-M. Mass spectrometry-based label-free quantitative proteomics.. J. Biomed. Biotech. 2010, 840518.
    doi: 10.1155/2010/840518google scholar: lookup
  12. White NA, Moore JN, Trim CM. Mucosal alterations in experimentally induced small intestinal strangulation obstruction in ponies.. Am. J. Vet. Res. 41, 193-198.
  13. McCarthy RN, Hutchins DR. Survival rates and post-operative complications after equine colic surgery.. Aust. Vet. J. 65, 40-43.
  14. Yang H, Ganguly A, Cabral F. Megakaryocyte lineage-specific class VI β-tubulin suppresses microtubule dynamics, fragments microtubules and blocks cell division.. Cytoskeleton 68, 175-187.
  15. Daugaard M, Rohde M, Jäättelä M. The heat shock protein 70 family: highly homologous proteins with overlapping and distinct functions.. FEBS Lett. 581, 3702-3710.
  16. Gangloff SC, Zähringer U, Blondin C, Guenounou M, Silver J, Goyert SM. Influence of CD14 on ligand interactions between lipopolysaccharide and its receptor complex.. J. Immunol. 175, 3940-3945.
  17. Stead RH, Bienenstock J, Stanisz AM. Neuropeptide regulation of mucosal immunity.. Immunol. Rev. 100, 333-359.
  18. Bär F, Föh B, Pagel R, Schröder T, Schlichting H, Hirose M, Lemcke S, Klinger A, König P, Karsten CM, Büning J, Lehnert H, Fellerman K, Ibrahim SM, Sina C. Carboxypeptidase E modulates intestinal immune homeostasis and protects against experimental colitis in mice.. PLoS One 9, e102347.
    doi: 10.1371/journal.pone.0102347google scholar: lookup
  19. Norseen J, Hosooka T, Hammarstedt A, Yore MM, Kant S, Aryal P, Kiernan UA, Phillips DA, Maruyama H, Kraus BJ, Usheva A, Davis RJ, Smith U, Kahn BB. Retinol-binding protein 4 inhibits insulin signaling in adipocytes by inducing proinflammatory cytokines in macrophages through a c-Jun N-terminal kinase- and toll-like receptor 4-dependent and retinol-independent mechanism.. Mol. Cell. Biol. 32, 2010-2019.
  20. Farjo KM, Farjo RA, Halsey S, Moiseyev G, Ma JX. Retinol-binding protein 4 induces inflammation in human endothelial cells by an NADPH oxidase- and nuclear factor kappa B-dependent and retinol-independent mechanism.. Mol. Cell. Biol. 32, 5103-5115.
  21. Kotnik P, Fischer-Posovszky P, Wabitsch M. RBP4: a controversial adipokine.. Eur. J. Enodocrinol. 165, 703-711.
  22. Kanamori-Katayama M, Kaiho A, Ishizu Y, Okamura-Oho Y, Hino O, Abe M, Kishimoto T, Sekihara H, Nakamura Y, Suzuki H, Forrest ARR, Hayashizaki Y. LRRN4 and UPK3B are markers of primary mesothelial cells.. PLoS One 6, e25391.
    doi: 10.1371/journal.pone.0025391google scholar: lookup
  23. Mutsaers SE, Prele CM-A, Pengelly S, Herrick SE. Mesothelial cells and peritoneal homeostasis.. Fertil. Steril. 106, 1018-1024.
  24. Turner JR. Intestinal mucosal barrier function in health and disease.. Nat. Rev. Immunol. 9, 799-809.
  25. Farshchian M, Kivisaari A, Ala-aho R, Riihilä P, Kallajoki M, Grénman R, Peltonen J, Pihlajaniemi T, Heljasvaara R, Kääri V-M. Serpin peptidase inhibitor clade A member 1 (SerpinA1) is a novel biomarker for progression of cutaneous squamous cell carcinoma.. Am. J. Pathol. 179, 1110-1119.
  26. Greene CM, Marciniak SJ, Teckman J, Ferrarotti I, Brantly ML, Lomas DA, Stoller JK, McElvaney NG. α-1 antitrypsin deficiency.. Nat. Rev. 2, 1-17.
  27. Fukaya Y, Shimada H, Wang L-C, Zandi E, DeClerk YA. Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma.. J. Biol. Chem. 283, 18573-18581.
  28. Jiang K, Rankin CR, Nava P, Sumagin R, Kamekura R, Stowell SR, Feng M, Parkos CA, Nusrat A. Galectin-3 regulates desmoglein-2 and intestinal epithelial intercellular adhesion.. J. Biol. Chem. 289, 10510-10517.
  29. Hsu DK, Yang R-Y, Pan Z, Yu L, Salomon DR, Fung-Leung W-P, Liu F-T. Targetted disruption of the Galectin-3 gene results in attenuated inflammatory responses.. Am. J. Pathol. 156, 1073-1083.
  30. Lund J, Olsen OH, Sørensen ES, Stennicke HR, Petersen HH, Overgaard MT. ADAMDEC1 is a metzincin metalloprotease with dampened proteolytic activity.. J. Biol. Chem. 288, 21367-21375.
  31. O'Shea NR, Chew TS, Dunne J, Marnane R, Nedjat-Shokouhi B, Smith PJ, Bloom SL, Smith AM, Segal AW. Critical role of the disintegrin metalloprotease ADAM-like Decysin-1 [ADAMDEC1] for intestinal immunity and inflammation.. J. Crohns Colitis 10, 1417-1427.
  32. Hazell GGJ, Peachey AMG, Teasdale JE, Sala-Newby GB, Angelini GD, Newby AC, White SJ. PI16 is a shear stress and inflammation-regulated inhibitor of MMP2.. Sci. Rep. 6, 39553.
    doi: 10.1038/srep39553google scholar: lookup
  33. Praissman JL, Live DH, Wang S, Ramiah A, Chinoy ZS, Boons G-J, Moremen K, Wells L. B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of α-dystroglycan.. eLife 3, e03943.
    doi: 10.7554/elife.03943google scholar: lookup
  34. Mathew G, Mitchell A, Down JM, Jacobs LA, Hamdy FC, Eaton C, Rosario DJ, Cross SS, Winder SJ. Nuclear targeting of dystroglycan promotes the expression of androgen regulated transcription factors in prostate cancer.. Sci. Rep. 3, 2792.
  35. Driss A, Charrier L, Yan Y, Nduati V, Sitaraman S, Merlin D. Dystroglycan receptor is involved in integrin activation in intestinal epithelia.. Am. J. Physiol. Gastrointest. Liver Physiol. 290, G1228-G1242.
  36. Stolen CM, Yegutkin GG, Kurkijärvi R, Bono O, Alitalo K, Jalkanen S. Origins of serum semicarbazide-sensitive amine oxidase.. Circ. Res. 95, 50-57.
  37. Luk GD, Bayless TM, Baylin SB. Diamine oxidase (histaminase). A circulating marker for rat intestinal mucosal maturation and integrity.. J. Clin. Invest. 66, 66-70.
  38. Zuliani-Alvarez L, Marzeda AM, Deligne C, Schwenzer A, McCann FE, Marsden BD, Piccinini AM, Midwood KS. Mapping tenascin-C interaction with toll-like receptor 4 reveals a new subset of endogenous inflammatory triggers.. Nat. Commun. 8, 1595.
    doi: 10.1038/s41467-017-01718-7google scholar: lookup
  39. Valcourt U, Alcaraz LB, Exposito J-Y, Lethias C, Bartholin L. Tenascin-X: beyond the architectural function.. Cell Adh. Migr. 9, 154-165.
  40. Liu Q, Zhou J-P, Li B, Huang Z-C, Dong H-Y, Li G-Y, Zhou K, Nie S-L. Basic transcription factor 3 is involved in gastric cancer development and progression.. World J. Gastroenterol. 19, 4495-4503.
  41. Jung T, Grune T. The proteasome and the degradation of oxidised proteins: part 1 - structure of proteasomes.. Redox. Biol. 1, 178-182.

Citations

This article has been cited 1 times.
  1. Ludwig EK, Hobbs KJ, McKinney-Aguirre CA, Gonzalez LM. Biomarkers of Intestinal Injury in Colic.. Animals (Basel) 2023 Jan 7;13(2).
    doi: 10.3390/ani13020227pubmed: 36670767google scholar: lookup
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