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Home / Equine Research Bank / Polymers (Basel) / Differential Biodegradation Kinetics of Collagen Membranes f...
Polymers2020; 12(6); 1290; doi: 10.3390/polym12061290

Differential Biodegradation Kinetics of Collagen Membranes for Bone Regeneration.

Abstract: Native collagen-based membranes are used to guide bone regeneration; but due to their rapid biodegradation, this treatment is often unpredictable. The purpose of this study was to investigate the biodegradability of natural collagen membranes. Three non-cross-linked resorbable collagen barrier membranes were tested: Derma Fina (porcine dermis), Evolution Standard (equine pericardium) and Duo-Teck (equine lyophilized collagen felt). 10 × 10 mm pieces of membranes were submitted to three different degradation procedures: (1) hydrolytic degradation in phosphate buffer solution, (2) enzyme resistance, using a 0.25% porcine trypsin solution, and (3) bacterial () collagenase resistance test. Weight measurements were performed with an analytic microbalance. Thickness was measured with a digital caliper. Membranes were analyzed at different time-points, up to 21 d of immersion. A stereomicroscope was used to obtain membranes' images. ANOVA and Student Newman Keuls were used for mean comparisons ( < 0.05), except when analyzing differences between time-points within the same membrane and solution where pair-wise comparisons were applied ( < 0.001). Derma Fina attained the highest resistance to all degradation challenges. Duo-Teck was the most susceptible membrane to degradation, complete degradation occurred as soon as 8 h. The bacterial collagenase solution performed as the most aggressive test as all membranes presented 100% degradation before 21 d.
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Publication Date: 2020-06-04 PubMed ID: 32512861PubMed Central: PMC7362079DOI: 10.3390/polym12061290Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the rate of biodegradability of different types of collagen membranes used in bone regeneration procedures. The findings could potentially improve the predictability and effectiveness of such treatments.

Research Objective and Methodology

  • The goal of this study was to explore how quickly different types of native collagen-based membranes biodegrade. The breakdown of these membranes is a common issue in bone regeneration treatments, often leading to unpredictable outcomes.
  • The researchers used three types of non-cross-linked resorbable collagen barrier membranes for their study: Derma Fina (made from pig skin), Evolution Standard (from horse pericardium), and Duo-Teck (from lyophilized equine collagen felt).
  • Each of these membranes was tested under three different degradation procedures: hydrolytic degradation in phosphate buffer solution, resistance to a 0.25% porcine trypsin solution (enzyme resistance), and resistance to a bacterial collagenase solution.

Measurement and Analysis

  • The researchers cut the membranes into 10mm x 10mm pieces and then subjected them to the various degradation procedures. They measured the weight of the membranes using an analytic microbalance and their thickness with a digital caliper.
  • The membranes were observed and assessed after exposure to the degradation solutions at different time points, ranging up to 21 days. A stereomicroscope was used to capture images of the membranes throughout the process.
  • The researchers then analyzed the data, using ANOVA and the Student Newman Keuls test to compare mean values, except when comparing differences over time within the same membrane and solution – in those cases, pair-wise comparisons were applied.

Research Findings

  • The study found that Derma Fina showed the greatest resistance to all of the degradation challenges.
  • Conversely, Duo-Teck was the most susceptible to degradation, with complete breakdown occurring as soon as 8 hours into the process.
  • All of the membranes experienced 100% degradation before the 21-day mark when exposed to the bacterial collagenase solution, demonstrating this to be the most effective at causing degradation.

Cite This Article

APA
Toledano M, Asady S, Toledano-Osorio M, García-Godoy F, Serrera-Figallo MA, Benítez-García JA, Osorio R. (2020). Differential Biodegradation Kinetics of Collagen Membranes for Bone Regeneration. Polymers (Basel), 12(6), 1290. https://doi.org/10.3390/polym12061290

Publication

Polymers
ISSN: 2073-4360
NlmUniqueID: 101545357
Country: Switzerland
Language: English
Volume: 12
Issue: 6
PII: 1290

Researcher Affiliations

Toledano, Manuel
  • Faculty of Dentistry, Dental Materials Section,University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain.
Asady, Samara
  • Faculty of Dentistry, Dental Materials Section,University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain.
Toledano-Osorio, Manuel
  • Faculty of Dentistry, Dental Materials Section,University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain.
García-Godoy, Franklin
  • College of Dentistry, Health Science Center,University of Tennessee, 875 Union Avenue, Memphis, TN 381632110, USA.
Serrera-Figallo, María-Angeles
  • Faculty of Dentistry, Oral Surgery Section, University of Sevilla, Avicena s/n, 41009 Sevilla, Spain.
Benítez-García, José A
  • Faculty of Dentistry, Dental Materials Section,University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain.
Osorio, Raquel
  • Faculty of Dentistry, Dental Materials Section,University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain.

Grant Funding

  • MINECO/FEDER MAT2017-85999P / Ministerio de Economu00eda, Industria y Competitividad, Gobierno de Espau00f1a
  • MINECO/FEDER MAT2017-85999P / European Regional Development Fund

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 16 times.
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