Differentiation molecules of the equine trophoblast.
Abstract: Monoclonal antibodies raised against horse placenta were tested using an indirect immunoperoxidase-labelling technique for reactivity with a panel of tissues from adult horses and conceptuses of various gestational ages. The pattern of reactivity of 4 of the antibodies (F67.1, F71.3, F71.7, F71.14) on trophoblastic tissues described unique antigenic phenotypes for the non-invasive trophoblast of the allantochorion, the invasive trophoblast of the chorionic girdle, and the mature endometrial cup cells, which are derived from the chorionic girdle. Two of the monoclonal antibodies (F67.1 and F71.3) reacted only with chorionic girdle and the endometrial cups. Antibody F71.7 labelled strongly the non-invasive allantochorion from Day 29 of gestation to term. However, F71.7 failed to label mature endometrial cups and stained chorionic girdle only weakly, suggesting that the ability of the girdle cells to synthesize the molecule identified by F71.7 was gradually lost after development of the girdle. Antibody F71.14 reacted with trophoblastic tissues from all stages of gestation tested, with the exception of chorionic girdle. The other 3 anti-trophoblast monoclonal antibodies (F71.1, F71.2 and F71.8) labelled trophoblast-derived tissues from all stages tested. When the monoclonal antibodies were tested on cultured fetal and placental cells from Day 33 conceptuses recovered non-surgically from pregnant mares, the reactivities of the monoclonal antibodies on cultured cells were mostly identical to their reactivities in situ on tissue samples of similar gestational age; F67.1 and F71.3 were strong, specific markers for chorionic girdle cells, and F71.7 labelled allantochorion weakly in vitro, but failed to label chorionic girdle cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1987-01-01 PubMed ID: 3479591
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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This research article investigates the reactivity pattern of monoclonal antibodies raised against horse placenta. These antibodies show different responses depending on the type of trophoblast cells (non-invasive/allantochorion, invasive/chorionic girdle, or mature endometrial cup cells) in horses at varied gestational ages. The outcomes provide unique insights into the distinct antigenic phenotypes of these cells.
Research Methodology
- The researchers used an indirect immunoperoxidase-labelling technique to test the reactivity of monoclonal antibodies raised against the horse placenta.
- Tissue samples were taken from adult horses and conceptuses of different gestational ages.
- The antibodies included in the study were F67.1, F71.3, F71.7, and F71.14, each showing a unique pattern of reactivity with the different trophoblastic tissues.
Key Findings
- Antibodies F67.1 and F71.3 only reacted with the invasive trophoblast of the chorionic girdle and the mature endometrial cup cells.
- Antibody F71.7 labelled the non-invasive trophoblast of the allantochorion strongly, but it did not label mature endometrial cups and only weakly labelled the chorionic girdle.
- This weak staining of the chorionic girdle by F71.7 suggests that the ability to synthesize the molecule identified by the antibody was gradually lost after the girdle’s development.
- Antibody F71.14 reacted with trophoblast tissues from all stages of gestation, except the chorionic girdle.
- The other three antibodies (F71.1, F71.2, and F71.8) labelled all tested trophoblast-derived tissues.
Lab Experiments on Cultured Cells
- The research team also tested the reactivity of these antibodies on cultured fetal and placental cells obtained non-surgically from pregnant mares on the 33rd day of their gestational periods.
- The observed reactivities were mostly consistent with the reactivities seen in situ on tissue samples of corresponding gestational ages.
- Two antibodies, F67.1 and F71.3, served as strong and specific markers for chorionic girdle cells, while F71.7 weakly labelled allantochorion in vitro but did not label chorionic girdle cells.
Cite This Article
APA
Antczak DF, Oriol JG, Donaldson WL, Poleman C, Stenzler L, Volsen SG, Allen WR.
(1987).
Differentiation molecules of the equine trophoblast.
J Reprod Fertil Suppl, 35, 371-378.
Publication
Researcher Affiliations
- James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Ithaca 14853.
MeSH Terms
- Animals
- Antibodies, Monoclonal
- Antigens, Differentiation / analysis
- Cells, Cultured
- Female
- Gestational Age
- Horses / embryology
- Placenta / immunology
- Pregnancy
- Trophoblasts / immunology
Grant Funding
- HD-15799 / NICHD NIH HHS
Citations
This article has been cited 6 times.- Brosnahan MM, Silvela EJ, Crumb J, Miller DC, Erb HN, Antczak DF. Ectopic Trophoblast Allografts in the Horse Resist Destruction by Secondary Immune Responses. Biol Reprod 2016 Dec;95(6):135.
- Wang X, Miller DC, Harman R, Antczak DF, Clark AG. Paternally expressed genes predominate in the placenta. Proc Natl Acad Sci U S A 2013 Jun 25;110(26):10705-10.
- Wang X, Miller DC, Clark AG, Antczak DF. Random X inactivation in the mule and horse placenta. Genome Res 2012 Oct;22(10):1855-63.
- Brosnahan MM, Miller DC, Adams M, Antczak DF. IL-22 is expressed by the invasive trophoblast of the equine (Equus caballus) chorionic girdle. J Immunol 2012 May 1;188(9):4181-7.
- de Mestre AM, Miller D, Roberson MS, Liford J, Chizmar LC, McLaughlin KE, Antczak DF. Glial cells missing homologue 1 is induced in differentiating equine chorionic girdle trophoblast cells. Biol Reprod 2009 Feb;80(2):227-34.
- Thompson RE, Meyers MA, Palmer J, Veeramachaneni DNR, Magee C, de Mestre AM, Antczak DF, Hollinshead FK. Production of Mare Chorionic Girdle Organoids That Secrete Equine Chorionic Gonadotropin. Int J Mol Sci 2023 May 31;24(11).
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