Dimeric inhibin concentrations in mares with granulosa-theca cell tumors.
Abstract: To determine whether concentrations of dimeric inhibin (CaCA) are greater in plasma and tumor fluid from mares with granulosa-theca cell tumors (GTCT), compared with concentrations in plasma and equine follicular fluid (eFF) from control mares. Methods: 6 mares with GTCT and 12 clinically normal mares. Methods: The alphabetaA immunoradiometric assay used 2 antibodies, one against each subunit of inhibin (alpha and betaA subunits). Tumor tissue, tumor fluid, and a single blood sample were collected at the time of surgical removal of the GTCT. A single blood sample was collected from 7 control mares during various stages of the estrous cycle. Five other control mares were ovariectomized when their ovaries contained growing follicles of 25 to 35 mm in diameter. A blood sample and eFF from the largest follicle were collected at the time of ovariectomy. Results: Mares with GTCT had significantly greater plasma concentrations of betabetaA (mean +/- SEM, 0.86 +/- 0.53 ng of recombinant human-alphabetaA/ml), compared with control mares (0.14+/-0.02 ng/ml). Concentrations of alphabetaA in tumor fluid and eFF were similar. Concentrations of alphabetaA were significantly lower after ovariectomy. Conclusions: Dimeric inhibin concentration was higher in plasma from mares with GTCT than in plasma from control mares. Increased granulosa cell mass and loss of mechanisms regulating alphabetaA release in mares with GTCT likely accounted for the increase in plasma concentrations. Measurement of alphabetaA concentrations may be useful for identifying mares with GTCT.
Publication Date: 1999-11-24 PubMed ID: 10566817
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research article investigates a possible correlation between high concentrations of the hormone known as dimeric inhibin in the blood and ovarian fluid of mares with a specific type of ovarian tumor. The paper suggests that this hormone concentration could be used as a diagnostic tool for the disease.
Methodology
- The study involved 6 mares with granulosa-theca cell tumors (GTCT), and 12 clinically healthy mares for comparison.
- A specific testing method, the alphabetaA immunoradiometric assay, was used to analyze the concentrations of inhibin. This method uses two antibodies, attacking each subunit of the inhibin hormone (alpha and betaA).
- For mares with GTCT, tumor tissue, tumor fluid, and a single blood sample were collected during the surgical removal of the tumor.
- Blood samples were taken from 7 healthy mares at various stages of their reproductive cycle as controls.
- Additionally, five healthy mares were ovariectomized when their ovaries contained growing follicles. A blood sample and fluid from the largest follicle were collected at the time of ovariectomy.
Results
- The results indicated that mares with GTCTs had significantly higher plasma concentrations of one subtype of dimeric inhibin (betabetaA).
- The concentrations of another subtype of inhibin (alphabetaA) in tumor fluid and in fluid from healthy equine follicles were found to be similar.
- After ovariectomy, the concentration of alphabetaA significantly decreased.
Conclusion
- The authors concluded that the concentration of dimeric inhibin was higher in the blood of mares with GTCTs than in the blood of the healthy control mares.
- This increase in inhibin concentration was attributed to the increased mass of granulosa cells (a type of cell in the ovaries), and a loss of regulatory mechanisms controlling the release of inhibin in horses with GTCTs.
- The researchers suggest that measuring the concentration of alphabetaA inhibin could be a useful method for identifying mares with GTCT.
Cite This Article
APA
Christman SA, Bailey MT, Wheaton JE, Troedsson MH, Ababneh MM, Santschi EM.
(1999).
Dimeric inhibin concentrations in mares with granulosa-theca cell tumors.
Am J Vet Res, 60(11), 1407-1410.
Publication
Researcher Affiliations
- Department of Animal Science, University of Minnesota, St Paul 55108, USA.
MeSH Terms
- Animals
- Dimerization
- Female
- Horse Diseases / blood
- Horse Diseases / metabolism
- Horses
- Immunoradiometric Assay
- Inhibins / analysis
- Inhibins / blood
- Luteoma / blood
- Luteoma / chemistry
- Luteoma / veterinary
- Ovarian Neoplasms / blood
- Ovarian Neoplasms / chemistry
- Ovarian Neoplasms / veterinary
- Ovariectomy
- Peptides / analysis
- Prostatic Secretory Proteins
- Reference Values
Citations
This article has been cited 5 times.- Frederico LM, Gerard MP, Pinto CR, Gradil CM. Bilateral occurrence of granulosa-theca cell tumors in an Arabian mare. Can Vet J 2007 May;48(5):502-5.
- Maurice KT. Diagnosis and surgical removal of a granulosa-theca cell tumor in a mare. Can Vet J 2005 Jul;46(7):644-6.
- Marino G, Nicòtina PA, Catone G, Bontempo RA, Zanghì A. Alpha-inhibin expression in canine ovarian neoplasms: preliminary results. Vet Res Commun 2003 Sep;27 Suppl 1:237-40.
- Curtin DJ. Ovarian hematoma in an 11-year-old Thoroughbred-Hanovarian mare. Can Vet J 2003 Jul;44(7):589-91.
- Tommasa SD, Roth SP, Triebe T, Brehm W, Lohmann KL, Stöckle SD. Successful intra-abdominal resection of a 24 kg ovarian granulosa cell tumor in a Warmblood mare. Open Vet J 2023 Sep;13(9):1212-1218.
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