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Home / Equine Research Bank / Vet Microbiol / Diversity of CTX-M-positive Escherichia coli recovered from ...
Veterinary microbiology2019; 231; 71-75; doi: 10.1016/j.vetmic.2019.02.031

Diversity of CTX-M-positive Escherichia coli recovered from animals in Canada.

Abstract: Historically, extended-spectrum cephalosporin resistance in bacteria from animals in Canada has been attributed to the SHV and CMY β-lactamase families. This pattern is beginning to change with the emergence of the bla gene family among Escherichia coli recovered from various animal species. Here we analyze and compare whole genome sequences of bla-positive E. coli isolates (n = 173) from dogs, chicken, swine, horses and beef cattle in Canada. Ten bla variants were identified with bla and bla being identified in most animal species. These variants occurred across many sequence types, suggesting that mobile genetic elements mediate the spread of bla. The variants bla and bla are associated with the global spread of bla in human clinical isolates and their presence could be indicative of transfer between humans and animals. These variants were also the principal variants identified among sequence type 131 isolates, which were not associated with any other species than dogs. These isolates carried the same bla variants as E. coli isolates found in humans. Close contact may promote the transmission of these isolates between humans and companion animals.
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
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Publication Date: 2019-02-23 PubMed ID: 30955827DOI: 10.1016/j.vetmic.2019.02.031Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study analyzes the emerging pattern of resistance in Escherichia coli bacteria, particularly observed in animals across Canada, where a shift is seen from the SHV and CMY β-lactamase families to the bla gene family. The research presents findings based on whole genome sequencing of bla-positive E. coli isolates across various animals.

Introduction and Background

  • The study investigates the rise in resistance in E. coli isolates, identified in a variety of animals in Canada.
  • Traditionally, the resistance in these bacteria was associated with the SHV and CMY β-lactamase families.
  • However, recent trends show a shift towards the bla gene family acting as a source of this resistance.

Methodology

  • The researchers used whole genome sequencing to investigate E. coli isolates from different animals including dogs, chicken, swine, horses, and beef cattle.
  • A total of 173 bla-positive E. coli isolates were studied.

Findings

  • Across the isolates, ten bla variants were identified, with bla and bla occurring in most animal species.
  • The various bla variants were found across many sequence types, suggesting that mobile genetic elements help in the spread of bla.
  • Two variants, bla and bla have associations with the worldwide spread of bla in human clinical isolates.
  • The presence of bla and bla could indicate transfer between humans and animals.

Significant Conclusions

  • The principal variants of bla and bla were mainly found in isolates corresponding to sequence type 131, which were only found in dogs.
  • These particular isolates carried the same bla variants as E. coli isolates found in humans, indicating a potential transmission between humans and companion animals, especially dogs.
  • The study concludes that close contact may promote the transmission of these variants between humans and companion animals.

Cite This Article

APA
Cormier A, Zhang PLC, Chalmers G, Weese JS, Deckert A, Mulvey M, McAllister T, Boerlin P. (2019). Diversity of CTX-M-positive Escherichia coli recovered from animals in Canada. Vet Microbiol, 231, 71-75. https://doi.org/10.1016/j.vetmic.2019.02.031

Publication

Veterinary microbiology
ISSN: 1873-2542
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 231
Pages: 71-75
PII: S0378-1135(19)30005-7

Researcher Affiliations

Cormier, Ashley
  • Department of Pathobiology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
Zhang, Pauline L C
  • Department of Pathobiology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
Chalmers, Gabhan
  • Department of Pathobiology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
Weese, J Scott
  • Department of Pathobiology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
Deckert, Anne
  • Centre for Foodborne, Environmental and Zoonotic Infectious Diseases, Public Health Agency of Canada, 160 Research Lane - 103, Guelph, Ontario, N1G 5B2, Canada.
Mulvey, Michael
  • National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, R3E 3M4, Canada.
McAllister, Tim
  • Agriculture and Agri-food Canada, 5403-1 Avenue South, Lethbridge, Alberta, T1J 4B1, Canada.
Boerlin, Patrick
  • Department of Pathobiology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada. Electronic address: pboerlin@uoguelph.ca.

MeSH Terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Canada
  • Cattle / microbiology
  • Cephalosporin Resistance
  • Chickens / microbiology
  • Dogs / microbiology
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / veterinary
  • Escherichia coli Proteins / biosynthesis
  • Genetic Variation
  • Genome, Bacterial
  • Horses / microbiology
  • Humans
  • Microbial Sensitivity Tests
  • Plasmids
  • Swine / microbiology
  • Whole Genome Sequencing
  • beta-Lactamases / biosynthesis

Citations

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