Effect of a specific platelet-activating factor antagonist on cardiovascular and peripheral cellular responses to colonic ischemia and reperfusion in anesthetized ponies.
Abstract: The role of platelet-activating factor in mediating the cardiovascular and peripheral cellular responses to large-colon ischemia and reperfusion, was explored in anesthetized ponies. A specific platelet-activating factor (PAF) antagonist (WEB 2086) was administered to a group of 6 ponies, and another 6 ponies (controls) were given an equivalent volume of saline solution, prior to 1 hour of large-colon torsion. After correction of the torsion, ponies were monitored during the reperfusion period. Significant (P < 0.05) hypotension and metabolic acidosis developed in all ponies after correction of colonic torsion, cardiac index increased initially, but then decreased significantly (P < 0.05) over the study period. Mean times between correction of torsion and onset of cardiac failure and death were not different between groups. Significant (P < 0.05) thrombocytopenia developed during the reperfusion period in control ponies, but not in WEB-treated ponies. Blood leukocyte concentration in control ponies was more variable and significantly (P < 0.05) decreased immediately upon reperfusion, compared with that in WEB-treated ponies. We conclude that although the cardiovascular responses to colonic ischemia and reperfusion are not prevented by use of a specific PAF-antagonist, specific peripheral cellular responses are mediated by PAF.
Publication Date: 1993-03-01 PubMed ID: 8498751
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates the effects of a specific type of drug called a Platelet-Activating Factor (PAF) antagonist on responses in the cardiovascular system and cells in the extremities of anesthetized ponies, during a procedure of induced colonic ischemia and reperfusion. The outcomes indicate that while the drug did not impact the cardiovascular responses, it affected specific responses in the cells located in the peripheral areas.
Methods and Findings
- The researchers experimented on twelve anesthetized ponies. They induced a condition called large-colon torsion, which is a twist in the large colon causing interruption of blood flow (ischemia), followed by restoration of blood flow (reperfusion).
- They divided the ponies into two groups. One group of six ponies was given a specific PAF antagonist (a kind of drug that blocks the action of PAF, named WEB 2086). The other group (control group) of six ponies were given an equivalent volume of saline solution.
- The study involved monitoring the ponies during the reperfusion period. The onset and progression of two conditions – hypotension (low blood pressure) and metabolic acidosis (excessive acidity in the body fluids) – were observed in all ponies following colonic torsion correction.
- The cardiac index initially increased but then significantly decreased over the study duration.
- The time between the correction of torsion and the onset of cardiac failure and death showed no noticeable difference between the two groups.
- There was a significant drop in the platelet count (thrombocytopenia) during the reperfusion phase in the control ponies, which was not observed in the ponies treated with WEB 2086.
- Similarly, the concentration of white blood cells (leukocytes) in the control group ponies was seen to fall immediately upon reperfusion, compared to a more stable concentration in the WEB-treated ponies.
Conclusion
- The study concluded that using a PAF antagonist like WEB 2086 does not prevent cardiovascular responses to colonic ischemia and reperfusion.
- However, the drug appears to influence specific peripheral cellular responses, as indicated by the relative stability in platelet and leukocyte counts in the WEB 2086-treated ponies compared to the control group.
- This suggests that PAF plays a mediating role in these peripheral cellular reactions during colonic ischemia and reperfusion.
Cite This Article
APA
Wilson DV, Stick JA.
(1993).
Effect of a specific platelet-activating factor antagonist on cardiovascular and peripheral cellular responses to colonic ischemia and reperfusion in anesthetized ponies.
Am J Vet Res, 54(3), 443-448.
Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, Michigan State University, East Lansing 48824.
MeSH Terms
- Analysis of Variance
- Animals
- Azepines / pharmacology
- Blood Pressure / drug effects
- Body Temperature / drug effects
- Cardiac Output / drug effects
- Colon / blood supply
- Female
- Heart Rate / drug effects
- Hemodynamics / drug effects
- Horses
- Ischemia / physiopathology
- Leukocyte Count / drug effects
- Male
- Orchiectomy
- Platelet Activating Factor / antagonists & inhibitors
- Pulmonary Circulation / drug effects
- Reperfusion
- Triazoles / pharmacology
- Vascular Resistance / drug effects
Citations
This article has been cited 1 times.- Sharifi K, Mostaghni K, Maleki M, Badiei K. Ischaemia/reperfusion injury in experimentally induced abomasal volvulus in sheep. Vet Res Commun 2007 Jul;31(5):575-90.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
