Effect of antioxidants on preservation of motility,viability and acrosomal integrity of equine spermatozoa during storage at 5 degrees C.
Abstract: Preservation of liquid semen at 5 degrees C is an important technique in the breeding management of horses. Oxidative damage to spermatozoa during storage is a potential cause of the decline in motility and fertility during hypothermic storage of liquid semen. The objective of this study was to evaluate the use of water-soluble and lipid-soluble antioxidants to improve the maintenance of motility of equine spermatozoa at 5 degrees C during storage for 72 to 96 h. In Experiment 1, the effect of addition of catalase on the maintenance of motility, viability and acrosomal integrity was determined. Semen was collected, and these treatments were applied: catalase (0, 100 or 200 U/mL) in nonfat, dried skim milk extender (NFDSM; with or without seminal plasma) or 10% seminal plasma + NFDSM. Motility was determined by computerized semen analysis (CASA) at 0, 24, 48 and 72 h. Viability and acrosomal integrity were determined at 72 h of storage. There was no significant treatment effect on the maintenance of sperm motility during 72 h storage. In Experiment 2, the effect of adding lipid-soluble antioxidants on maintenance of motility was evaluated. Semen was diluted to a final concentration of 25 x 10(6) sperm/mL in NFDSM containing butylated hydroxytoluene (BHT; 2.0, 1.0, or 0.5 mM), Vitamin E (4.0, 2.0, 1.0 mM), or Tempo (2.0, 1.0, or 0.5 mM). Although the addition of BHT significantly reduced (P < 0.05) progressive motility during storage compared to the control, there were no positive treatment effects of either Vitamin E or Tempo on maintenance of motility. In Experiment 3, the effect of adding water-soluble antioxidants on maintenance of motility was evaluated. Semen was diluted in NFDSM containing these treatments: Trolox (2.0 mM), Tempo (1.0 mM), Vitamin C (0.45 mg/mL), BSA (3% w/v), combinations of these antioxidants, or control. Adding these water-soluble antioxidants did not significantly improve the maintenance of motility during cooled storage at 5 degrees C. In conclusion, adding the enzyme scavenger, catalase, or a variety of lipid- and water-soluble antioxidants did not significantly improve the maintenance of motility during liquid semen storage at 5 degrees C.
Publication Date: 2001-09-27 PubMed ID: 11572439DOI: 10.1016/s0093-691x(01)00590-8Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research study explores the impact of antioxidants on the preservation of equine spermatozoa motility, viability, and acrosomal integrity during storage at 5 degrees Celsius. The investigation revealed that adding antioxidants or the enzyme scavenger (catalase) had no significant effect on maintaining motility during storage.
Research Purpose and Objectives
- The primary purpose of this study was to understand the effect of various antioxidants on preserving the motility, viability, and acrosomal integrity of horse sperm at low temperatures.
- The study aimed to improve the techniques of semen preservation, which are critical in horse breeding management.
- The paper also sought to mitigate the oxidative damage that potentially causes a decline in sperm motility and fertility during hypothermic storage.
Methodology
- The study was carried out using three different experiments over a period of 72 to 96 hours, with sperm stored at 5 degrees Celsius.
- The first experiment evaluated the effect of adding catalase (parenteral antioxidant enzyme) to the semen. Measurements of motility, viability, and acrosomal integrity were taken after catalase treatments.
- In the second experiment, the emphasis was on understanding how the inclusion of lipid-soluble antioxidants (i.e., Butylated Hydroxytoluene, Vitamin E, and Tempo) affected the maintenance of motility.
- The third experiment focused on water-soluble antioxidants (Trolox, Tempo, Vitamin C, and BSA) and their impact on sperm motility.
- After the addition of these substances, motility was evaluated using computerized semen analysis at different time intervals during the storage period.
Key Findings
- The study found no significant impact of the addition of catalase on maintaining motility, viability, and acrosomal integrity in the first experiment.
- The second experiment revealed that adding lipid-soluble antioxidants did not offer any positive treatment effect in maintaining sperm motility, and BHT even reduced progressive motility during storage.
- In the third experiment, it was determined that the introduction of water-soluble antioxidants did not significantly improve the maintenance of sperm motility during storage at 5 degrees Celsius.
- Overall, the study revealed that the addition of a variety of lipid- and water-soluble antioxidants or enzyme scavengers did not have a significant effect on preserving the motility of equine spermatozoa during storage at 5 degrees Celsius.
Cite This Article
APA
Ball BA, Medina V, Gravance CG, Baumbe J.
(2001).
Effect of antioxidants on preservation of motility,viability and acrosomal integrity of equine spermatozoa during storage at 5 degrees C.
Theriogenology, 56(4), 577-589.
https://doi.org/10.1016/s0093-691x(01)00590-8 Publication
Researcher Affiliations
- Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis 95616, USA. baball@ucdavis.edu
MeSH Terms
- Acrosome / drug effects
- Acrosome / physiology
- Animals
- Antioxidants / pharmacology
- Bisbenzimidazole / chemistry
- Catalase / analysis
- Catalase / pharmacology
- Cold Temperature
- Fluorescent Dyes / chemistry
- Horses / physiology
- Male
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sperm Motility / drug effects
- Sperm Motility / physiology
- Spermatozoa / drug effects
- Spermatozoa / physiology
Citations
This article has been cited 14 times.- Aguiar CS, Barros CHSC, Machado WM, Allaman IB, Leite AO, Barbosa LP, Snoeck PPDN. Effect of different concentrations of Trolox(®) in association with docosahexaenoic acid on equine semen freezing. Anim Reprod 2022;19(4):e20220010.
- Mohammadi H, Golbabaei F, Dehghan SF, Imani H, Ramezani Tehrani F, Khodakarim Ardakani S. The Influence of Vitamin E and Omega-3 Fatty Acids on Reproductive Health Indices Among Male Workers Exposed to Electromagnetic Fields. Am J Mens Health 2022 Jan-Feb;16(1):15579883221074821.
- Partyka A, Niżański W. Supplementation of Avian Semen Extenders with Antioxidants to Improve Semen Quality-Is It an Effective Strategy?. Antioxidants (Basel) 2021 Nov 30;10(12).
- Moradi M, Hajarian H, Karamishabankareh H, Soltani L, Soleymani B. Pre-treatment of ram semen extender with magnetic nanoparticles on freeze-thawed spermatozoa. Vet Med Sci 2022 Mar;8(2):792-798.
- Silvestre MA, Yániz JL, Peña FJ, Santolaria P, Castelló-Ruiz M. Role of Antioxidants in Cooled Liquid Storage of Mammal Spermatozoa. Antioxidants (Basel) 2021 Jul 8;10(7).
- Saadeldin IM, Khalil WA, Alharbi MG, Lee SH. The Current Trends in Using Nanoparticles, Liposomes, and Exosomes for Semen Cryopreservation. Animals (Basel) 2020 Dec 3;10(12).
- Abbaszadeh S, Farrokhi-Ardabili F, Malekinejad H, Bernousi I. Alpha-zearalenol negatively influences ram sperm parameters during liquid storage. Vet Res Forum 2018 Spring;9(2):171-178.
- Hamerezaee M, Dehghan SF, Golbabaei F, Fathi A, Barzegar L, Heidarnejad N. Assessment of Semen Quality among Workers Exposed to Heat Stress: A Cross-Sectional Study in a Steel Industry. Saf Health Work 2018 Jun;9(2):232-235.
- Patel A, Saxena A, Swain DK, Yadav D, Yadav SS, Kumar A, Kumar A. Effect of supplementation of butylated hydroxytoluene on post-thaw sperm viability, motility and membrane integrity of Hariana bulls. Vet World 2015 Jun;8(6):808-12.
- Plaza Davila M, Martin Muñoz P, Tapia JA, Ortega Ferrusola C, Balao da Silva C C, Peña FJ. Inhibition of Mitochondrial Complex I Leads to Decreased Motility and Membrane Integrity Related to Increased Hydrogen Peroxide and Reduced ATP Production, while the Inhibition of Glycolysis Has Less Impact on Sperm Motility. PLoS One 2015;10(9):e0138777.
- Gil L, Galindo-Cardiel I, Malo C, González N, Alvarez C. Effect of Cholesterol and Equex-STM Addition to an Egg Yolk Extender on Pure Spanish Stallion Cryopreserved Sperm. ISRN Vet Sci 2013;2013:280143.
- Li SF, Liu HX, Zhang YB, Yan YC, Li YP. The protective effects of alpha-ketoacids against oxidative stress on rat spermatozoa in vitro. Asian J Androl 2010 Mar;12(2):247-56.
- Chanapiwat P, Kaeoket K, Tummaruk P. Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen. Asian J Androl 2009 Sep;11(5):600-8.
- Shalaby FM, Hassan SA, El-Raghi AA, Alajmi FE, Alfayoumi MG, Attia KAEH. The beneficial impact of pomegranate oil nanoemulsion on the quality of cryopreserved bovine sperm: antioxidant and anti-apoptotic effects. Anim Biosci 2025 Sep;38(9):1894-1905.
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