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Effect of insemination dose and site on uterine inflammatory response of mares.
Abstract: It is unclear whether AI of mares deep into the uterine horn causes more or less inflammation of the endometrium than conventional AI. Thus, we compared uterine inflammatory reactions of mares inseminated with two different doses of frozen-thawed semen into the tip of the uterine horn (UH) ipsilateral to the preovulatory follicle with those of mares inseminated into the uterine body (UB). Thirty-two mares were assigned to one of four groups (eight mares/group): UB20=AI into UB, 20 x 10(6)sperm/0.5 mL; UB200=AI into UB, 200 x 10(6)sperm/0.5 mL; UH20=AI into UH, 20 x 10(6)sperm/0.5 mL; UH200=AI into UH, 200 x 10(6)sperm/0.5 mL, and inseminated 24 h after hCG administration. Before and 24 h after AI, they were examined with ultrasonography for the presence of intrauterine fluid. At 24 h, uterine fluid samples were obtained first by absorbing fluid into a tampon and then by uterine lavage. Uterine fluid was examined for polymorphonuclear leukocytes (PMN) and bacteriology, and frozen for lysozyme and TIC (trypsin-inhibitor capacity) assays. Only three mares conceived, one in each of the following groups: UB200, UH20, and UH200. Mares in the UH20 group accumulated less intrauterine fluid (p<0.05) than those in the other groups, which had similar amounts. No significant differences in PMN numbers were detected in either tampon or lavage fluid. Enzyme levels between groups did not differ statistically, except for TIC, which was lowest in the UH200 group. Thus, deep uterine horn AI caused no greater inflammation or irritation than uterine body AI in normal mares 24 h after insemination.
Publication Date: 2004-12-15 PubMed ID: 15910930DOI: 10.1016/j.theriogenology.2004.10.016Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article investigates the impact of the insemination dose and location on the inflammation of a mare’s uterus. The study finds that deep uterine horn artificial insemination doesn’t significantly differ in causing inflammation compared to uterine body artificial insemination 24 hours post-insemination.
Research Parameters and Methods
- All mares involved in the study were artificially inseminated and analyzed 24 hours post-insemination.
- A total of 32 mares were divided into four groups based on insemination location and dosage. Each group had 8 mares and two variables: insemination location (Uterine Body or UB and Uterine Horn or UH) and sperm amount (20 million sperm or 200 million sperm per 0.5 mL).
- All inseminations were performed with frozen-thawed semen.
- The mares were examined using ultrasonography for the presence of intrauterine fluid before and after artificial insemination.
- The researchers obtained uterine fluid samples 24 hours after AI, first by absorbing the fluid into a tampon, and then by uterine lavage.
Results and Findings
- Only three mares conceived, one from each of the following groups: UB200, UH20, and UH200, which indicates that the insemination site and dose did not significantly affect the conception rate.
- Mares in the UH20 group accumulated less intrauterine fluid when compared to the other groups.
- No significant differences were detected in the numbers of polymorphonuclear leukocytes (types of white blood cells) in tampon or lavage fluid, statistical differences weren’t noted for enzyme levels, except the trypsin-inhibitor capacity (TIC), which was lowest in the UH200 group
Conclusion
- The study concluded that artificial insemination in the deep uterine horn resulted in no greater inflammation or irritation than artificial insemination in the uterine body 24 hours after insemination.
- These findings suggest that the insemination site and dose do not have a major impact on inflammation or irritation in the uterine horn or body of mares.
Cite This Article
APA
Güvenc K, Reilas T, Katila T.
(2004).
Effect of insemination dose and site on uterine inflammatory response of mares.
Theriogenology, 63(9), 2504-2512.
https://doi.org/10.1016/j.theriogenology.2004.10.016 Publication
Researcher Affiliations
- Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Istanbul, 34851 Avcilar, Istanbul, Turkey.
MeSH Terms
- Animals
- Endometritis / epidemiology
- Endometritis / etiology
- Endometritis / veterinary
- Female
- Horse Diseases / epidemiology
- Horse Diseases / etiology
- Horses
- Insemination, Artificial / adverse effects
- Insemination, Artificial / methods
- Insemination, Artificial / veterinary
- Leukocyte Count
- Male
- Neutrophils
- Semen
Citations
This article has been cited 4 times.- Freccero F, Mislei B, Bucci D, Dondi F, Mari G. Effects of Intra-Uterine Fluid Accumulation after Artificial Insemination on Luteal Function in Mares. Animals (Basel) 2022 Dec 23;13(1).
- Katila T, Ferreira-Dias G. Evolution of the Concepts of Endometrosis, Post Breeding Endometritis, and Susceptibility of Mares. Animals (Basel) 2022 Mar 19;12(6).
- Christoffersen M, Woodward E, Bojesen AM, Jacobsen S, Petersen MR, Troedsson MH, Lehn-Jensen H. Inflammatory responses to induced infectious endometritis in mares resistant or susceptible to persistent endometritis. BMC Vet Res 2012 Mar 29;8:41.
- Maischberger E, Irwin J, Carrington S, Duggan V. Equine post-breeding endometritis: A review. Ir Vet J 2008 Mar 1;61(3):163-8.
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