Effect of nutraceutical supplementation on semen quality in stallions.

Du00edaz Rojas, E
·
Carrillo Moreno, DI
·
Contreras Villarreal, V
·
Arellano Rodru00edguez, F
·
Alvarado Espino, AS
·
u00c1ngel Garcu00eda, O

Abstract: The use of reproductive biotechnologies in equine practice has shown that some stallions are subfertile, so ways to improve fertility have been sought. This study aimed to evaluate the effect of nutraceutical supplementation on improving semen quality in Quarter Horse stallions. Semen from six Quarter Horse stallions was assessed for 4 months every 20 days using the computer-assisted semen analysis system. They were evaluated for 60 days before supplementation; then, the same stallions were re-evaluated for 60 days with nutraceutical supplementation (30 g/day). Volume showed no significant difference (p > 0.05) with nutraceuticals. Sperm concentration (10x ) was significantly higher with supplementation (339.4 ± 17.5 sperm/mL) than without supplementation (224.6 ± 19.9). Sperm abnormalities (%) were significantly (p < 0.05) lower with supplementation (14.3 ± 0.6) than without supplementation (19.1 ± 0.4). Sperm kinematic parameters, total motility (TM), progressive motility (PM), rectilinear velocity (VSL), the velocity of the trajectory (VAP) and curvilinear velocity (VCL), were significantly better with supplementation (p < 0.05). Based on the results, it is concluded that nutraceutical supplementation improved semen parameters in Quarter Horse stallions.

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Publication Date: 2023-10-10 PubMed ID: 37817488PubMed Central: PMC10650371DOI: 10.1002/vms3.1289Google Scholar: Lookup

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Conflict of Interest
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Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research analyzes the effect of nutraceutical supplementation on the semen quality of Quarter Horse stallions. Numerical results suggest that such supplementation improves sperm concentration and lowers sperm abnormalities, enhancing overall semen parameters.

Objectives and Methods

This study tackled the problem of some stallions exhibiting subfertility, which has been revealed by the growing use of reproductive biotechnologies.
To find a solution, the researchers decided to explore the potential of nutraceutical supplementation for improving semen quality.
The test subjects were six Quarter Horse stallions. Their semen was analyzed every 20 days over a duration of four months with the use of a computer-assisted semen analysis system.
Before nutraceutical supplementation, the stallions were evaluated for 60 days. After the initial evaluation, they were given the supplementation (30 g per day) and re-evaluated for another 60 days.

Results and Conclusions

At the end of the experiment, the semen volume of the test subjects didn’t show any noticeable change due to the nutraceutical supplementation.
However, the sperm concentration showed a significant increase. The concentration was around 339.4 sperm per mL with the supplementation, compared to 224.6 sperm per mL without the supplementation.
Furthermore, the percentage of abnormal sperm was significantly lower under the influence of the supplementation. It was around 14.3% with the supplements, compared to 19.1% without them.
Many other parameters related to sperm activity, like total motility, progressive motility, rectilinear velocity, the velocity of the trajectory, and curvilinear velocity, also improved with the supplementation.
From these findings, the researchers concluded that nutraceutical supplementation effectively improves semen parameters in Quarter Horse stallions, making it potentially useful for addressing the issue of equine subfertility.

Cite This Article

APA

Du00edaz Rojas E, Carrillo Moreno DI, Contreras Villarreal V, Arellano Rodru00edguez F, Alvarado Espino AS, u00c1ngel Garcu00eda O. (2023). Effect of nutraceutical supplementation on semen quality in stallions. Vet Med Sci, 9(6), 2600-2605. https://doi.org/10.1002/vms3.1289

Publication

Veterinary medicine and science

ISSN: 2053-1095

NlmUniqueID: 101678837

Country: England

Language: English

Volume: 9

Issue: 6

Pages: 2600-2605

Researcher Affiliations

Du00edaz Rojas, Edgar

Ciencias en Producciu00f3n Agropecuaria, Universidad Autu00f3noma Agraria Antonio Narro, Torreu00f3n, Coahuila, Mexico.

Carrillo Moreno, Dalia I

Departamento de Producciu00f3n Animal, Universidad Autu00f3noma Agraria Antonio Narro, Torreu00f3n, Coahuila, Mexico.

Contreras Villarreal, Viridiana

Departamento de Ciencias Mu00e9dico Veterinarias, Universidad Autu00f3noma Agraria Antonio Narro, Torreu00f3n, Coahuila, Mexico.

Arellano Rodru00edguez, Fernando

Departamento de Producciu00f3n Animal, Universidad Autu00f3noma Agraria Antonio Narro, Torreu00f3n, Coahuila, Mexico.

Alvarado Espino, Alan S

Departamento de Producciu00f3n Animal, Universidad Autu00f3noma Agraria Antonio Narro, Torreu00f3n, Coahuila, Mexico.

u00c1ngel Garcu00eda, Oscar

Departamento de Ciencias Mu00e9dico Veterinarias, Universidad Autu00f3noma Agraria Antonio Narro, Torreu00f3n, Coahuila, Mexico.

MeSH Terms

Horses
Male
Animals
Semen Analysis / veterinary
Semen
Sperm Motility
Semen Preservation / veterinary
Cryopreservation / veterinary
Dietary Supplements

Conflict of Interest Statement

All authors declare that there is no conflict of interest that could affect the integrity of the currently reported results.

References

This article includes 38 references

Aitken, R. J. , & Baker, M. A. (2004). Oxidative stress and male reproductive biology. Reproduction, Fertility and Development, 16(5), 581u2013588. 10.4103/1008-682X.122203
doi: 10.4103/1008-682X.122203pubmed: 15367373google scholar: lookup
Alamaary, M. S. , Haron, A. W. , Ali, M. , Hiew, M. W. H. , Adamu, L. , & Peter, I. D. (2019). Effects of four extenders on the quality of frozen semen in Arabian stallions. Veterinary World, 12(1), 34u201340. 10.14202/vetworld.2019.34-40
doi: 10.14202/vetworld.2019.34-40pmc: PMC6431823pubmed: 30936651google scholar: lookup
Alvarenga, M. A. , Papa, F. O. , & Ramires Neto, C. (2016). Advances in stallion semen cryopreservation. Veterinary Clinics: Equine Practice, 32(3), 521u2013530. 10.1016/j.cveq.2016.08.003
doi: 10.1016/j.cveq.2016.08.003pubmed: 27726989google scholar: lookup
Arruda, R. P. D. , Silva, D. F. D. , Alonso, M. A. , Andrade, A. F. C. D. , Nascimento, J. , Gallego, A. M. , Martins, S. M. M. K. , & Granato, T. M. (2010). Nutraceuticals in reproduction of bulls and stallions. Revista Brasileira de Zootecnia, 39, 393u2013400. 10.1590/s1516-35982010001300043
doi: 10.1590/s1516-35982010001300043google scholar: lookup
Aurich, C. (2008). Recent advances in cooledu2010semen technology. Animal Reproduction Science, 107(3u20134), 268u2013275. 10.1016/j.anireprosci.2008.04.015
doi: 10.1016/j.anireprosci.2008.04.015pubmed: 18524507google scholar: lookup
Aurich, J. , Kuhl, J. , Tichy, A. , & Aurich, C. (2020). Efficiency of semen cryopreservation in stallions. Animals, 10(6), 1033. 10.3390/ani10061033
doi: 10.3390/ani10061033pmc: PMC7341245pubmed: 32545785google scholar: lookup
Aurich, J. E. (2012). Artificial insemination in horsesu2014More than a century of practice and research. Journal of Equine Veterinary Science, 32(8), 458u2013463. 10.1016/j.jevs.2012.06.011
doi: 10.1016/j.jevs.2012.06.011google scholar: lookup
Bahrami, A. , Divar, M. R. , Azari, M. , & Kafi, M. (2020). Nicotinic acid (niacin) supplementation in cooling and freezing extenders enhances stallion semen characteristics. Journal of Equine Veterinary Science, 94, 103236. 10.1016/j.jevs.2020.103236
doi: 10.1016/j.jevs.2020.103236pubmed: 33077098google scholar: lookup
Bazzano, M. , Laus, F. , Spaterna, A. , & Marchegiani, A. (2021). Use of nutraceuticals in the stallion: Effects on semen quality and preservation. Reproduction in Domestic Animals, 56(7), 951u2013957. 10.1111/rda.13934
doi: 10.1111/rda.13934pmc: PMC8360198pubmed: 33772909google scholar: lookup
Brinsko, S. , Crockett, E. , & Squires, E. (2000). Effect of centrifugation and partial removal of seminal plasma on equine spermatozoa motility after cooling and storage. Theriogenology, 54(1), 129u2013136. 10.1016/s0093-691x(00)00331-9
doi: 10.1016/s0093-691x(00)00331-9pubmed: 10990354google scholar: lookup
Brinsko, S. P. , Varner, D. D. , Love, C. C. , Blanchard, T. L. , Day, B. C. , & Wilson, M. E. (2005). Effect of feeding a DHAu2010enriched nutraceutical on the quality of fresh, cooled, and frozen stallion semen. Theriogenology, 63(5), 1519u20131527. 10.1016/j.theriogenology.2004.07.010
doi: 10.1016/j.theriogenology.2004.07.010pubmed: 15725455google scholar: lookup
Campos, G. A. , Garcia, V. F. , Freitasu2010Dell'Aqua, C. P. , Segabinazzi, L. G. , Maciel, L. F. , Alvarenga, M. A. , Papa, F. O. , & Dell'Aqua, J. A. (2020). Sodium caseinate and cholesterol improve bad cooler stallion fertility. Journal of Equine Veterinary Science, 93, 103201. 10.1016/j.jevs.2020.103201
doi: 10.1016/j.jevs.2020.103201pubmed: 32972672google scholar: lookup
Carroll, C. L. , & Huntington, P. J. (1988). Body condition scoring and weight estimation of horses. Equine Veterinary Journal, 20(1), 41u201345. 10.1111/j.2042-3306.1988.tb01451.x
doi: 10.1111/j.2042-3306.1988.tb01451.xpubmed: 3366105google scholar: lookup
Contri, A. , De Amicis, I. , Molinari, A. , Faustini, M. , Gramenzi, A. , Robbe, D. , & Carluccio, A. (2011). Effect of dietary antioxidant supplementation on fresh semen quality in stallion. Theriogenology, 75(7), 1319u20131326. 10.1016/j.theriogenology.2010.12.003
doi: 10.1016/j.theriogenology.2010.12.003pubmed: 21295825google scholar: lookup
Dehdari Ebrahimi, N. , Parsa, S. , Nozari, F. , Shahlaee, M. A. , Maktabi, A. , Sayadi, M. , Sadeghi, A. , & Azarpira, N. (2023). Protective effects of melatonin against the toxic effects of environmental pollutants and heavy metals on testicular tissue: A systematic review and metau2010analysis of animal studies. Frontiers in Endocrinology, 14, 1119553. 10.3389/fendo.2023.1119553
doi: 10.3389/fendo.2023.1119553pmc: PMC9922902pubmed: 36793277google scholar: lookup
Deichsel, K. , Palm, F. , Koblischke, P. , Budik, S. , & Aurich, C. (2008). Effect of a dietary antioxidant supplementation on semen quality in pony stallions. Theriogenology, 69(8), 940u2013945. 10.1016/j.theriogenology.2008.01.007
doi: 10.1016/j.theriogenology.2008.01.007pubmed: 18358523google scholar: lookup
Del Prete, C. , Tafuri, S. , Ciani, F. , Pasolini, M. P. , Ciotola, F. , Albarella, S. , Carotenuto, D. , Peretti, V. , & Cocchia, N. (2018). Influences of dietary supplementation with Lepidium meyenii (Maca) on stallion sperm production and on preservation of sperm quality during storage at 5u00b0C. Andrology, 6(2), 351u2013361. 10.1111/andr.12463
doi: 10.1111/andr.12463pubmed: 29359412google scholar: lookup
El Sisy, G. A. , Abo Elu2010Maaty, A. M. , & Rawash, Z. M. (2016). Comparative blood and seminal plasma oxidant/antioxidant status of Arab stallions with different ages and their relation to semen quality. Asian Pacific Journal of Reproduction, 5(5), 428u2013433. 10.1016/j.apjr.2016.07.006
doi: 10.1016/j.apjr.2016.07.006google scholar: lookup
Freitas, M. L. , Bouu00e9res, C. S. , Pignataro, T. A. , Gonu00e7alves de Oliveira, F. J. , de Oliveira Viu, M. A. , & de Oliveira, R. A. (2016). Quality of fresh, cooled, and frozen semen from stallions supplemented with antioxidants and fatty acids. Journal of Equine Veterinary Science, 46, 1u20136. 10.1016/j.jevs.2016.07.003
doi: 10.1016/j.jevs.2016.07.003google scholar: lookup
Freitas, M. L. , & de Oliveira, R. A. (2018). Nutraceutical in male reproduction. Brazilian Journal of Veterinary Medicine, 40, 1u201310. 10.29374/2527-2179.bjvm220118
doi: 10.29374/2527-2179.bjvm220118google scholar: lookup
Govindaraj, M. , Masilamani, P. , Albert, V. A. , & Bhaskaran, M. (2017). Role of antioxidant in seed quality u2013 A review. Agricultural Reviews, 38(03), 180u2013190. 10.18805/ag.v38i03.8977
doi: 10.18805/ag.v38i03.8977google scholar: lookup
Grady, S. , Cavinder, C. , Brinsko, S. , Forrest, D. , Sawyer, J. , & Scott, B. (2009). Dietary supplementation of two varying sources of nu00a0u2212u00a03 fatty acids and subsequent effects on fresh, cooled, and frozen seminal characteristics of stallions. The Professional Animal Scientist, 25(6), 768u2013773. 10.15232/S1080-7446(15)30787-7
doi: 10.15232/S1080-7446(15)30787-7google scholar: lookup
Halliwell, B. , & Whiteman, M. (2004). Measuring reactive species and oxidative damage in vivo and in cell culture: How should you do it and what do the results mean. British Journal of Pharmacology, 142(2), 231u2013255. 10.1038/sj.bjp.0705776
doi: 10.1038/sj.bjp.0705776pmc: PMC1574951pubmed: 15155533google scholar: lookup
Houssou, H. , Bouzebdau2010Afri, F. , & Bouzebda, Z. (2020). Evaluation of sexual behavior of stallion (Arabian versus Barb) during breeding season in Algeria. Indian Journal of Animal Research, 54, 1078u20131082. 10.18805/ijar.B-950
doi: 10.18805/ijar.B-950google scholar: lookup
Imran, M. , Ghorat, F. , Ulu2010Haq, I. , Uru2010Rehman, H. , Aslam, F. , Heydari, M. , & Rebezov, M. (2020). Lycopene as a natural antioxidant used to prevent human health disorders. Antioxidants, 9(8), 706. 10.3390/antiox9080706
doi: 10.3390/antiox9080706pmc: PMC7464847pubmed: 32759751google scholar: lookup
nInstituto Nacional de Investigaciones Forestales Agru00edcolas y Pecuarias (INIFAP)n. (2017). nRed nacional de estaciones agrometeorolu00f3gicas automatizadasn. INIFAP; nhttps://clima.inifap.gob.mx/lnmysr/Estacionesn
Lanu00e7oni, R. , Celeghini, E. C. C. , Alves, M. B. R. , Lemes, K. M. , Gonellau2010Diaza, A. M. , Oliveira, L. Z. , & Arruda, R. P. D. (2018). Melatonin added to cryopreservation extenders improves the mitochondrial membrane potential of postthawed equine sperm. Journal of Equine Veterinary Science, 69, 78u201383. 10.1016/j.jevs.2018.06.006
doi: 10.1016/j.jevs.2018.06.006google scholar: lookup
Mongioi, L. , Calogero, A. E. , Vicari, E. , Condorelli, R. A. , Russo, G. I. , Privitera, S. , Morgia, G. , & La Vignera, S. (2016). The role of carnitine in male infertility. Andrology, 4(5), 800u2013807. 10.1111/andr.12191
doi: 10.1111/andr.12191pubmed: 27152678google scholar: lookup
Monteiro, G. A. , Ramires Neto, C. , Sancleru2010Silva, Y. F. R. , Oliveira, L. Z. , Aqua, C. P. F. D. , Hartwig, F. P. , Papa, P. M. , Pantoja, J. , Silva, D. F. , & Papa, F. O. (2019). Efeito da adiu00e7u00e3o de plasma seminal oriundo de animais de alta e baixa fertilidade na criopreservau00e7u00e3o de espermatozoides da cauda do epidu00eddimo e do ejaculado de garanhu00f5es subfu00e9rteis. Arquivo Brasileiro de Medicina Veterinu00e1ria e Zootecnia, 71(3), 752u2013760. 10.1590/1678-4162-10234
doi: 10.1590/1678-4162-10234google scholar: lookup
NAM . (2002). Guide for the care and use of laboratory animals. National Academy of Medicine, Mu00e9xico, and the Association for Assessment and Accreditation of Laboratory Animal Care International.
Nery, I. H. , Arau00fajo Silva, R. A. , Souza, H. M. , Arruda, L. C. , Monteiro, M. M. , Seal, D. C. , Silva, G. R. , Silva, T. M. , Carneiro, G. F. , Batista, A. M. , Cu00e2mara, D. R. , & Guerra, M. M. P. (2020). Effects of lu2010carnitine on equine semen quality during liquid storage. Biopreservation and Biobanking, 18(5), 403u2013408. 10.1089/bio.2020.0025
doi: 10.1089/bio.2020.0025pubmed: 32799560google scholar: lookup
nnRavi, S. K.n, nKumar, H.n, nVyas, S.n, nNarayanan, K.n, nKumari, S.n, nSingh, J.n, & nJan, M. H.n (2016). Effect of omegau20103 fatty acids enriched diet on semen characteristics in Marwari horses. Indian Journal of Animal Sciences, 86(6), 726u2013728. http://www.icar.org.inn
Rodrigues, P. G. , de Moura, R. S. , Rocha, L. G. P. , Bottino, M. P. , Nichi, M. , Maculan, R. , Bertechini, A. G. , & Souza, J. C. (2017). Dietary polyunsaturated fatty acid supplementation improves the quality of stallion cryopreserved semen. Journal of Equine Veterinary Science, 54, 18u201323. 10.1016/j.jevs.2016.08.007
doi: 10.1016/j.jevs.2016.08.007google scholar: lookup
Ruiz, A. J. , Tibary, A. , Heaton, R. A. , Hargreaves, I. P. , Leadon, D. P. , & Bayly, W. M. (2021). Effects of feeding coenzyme Q10u2010Ubiquinol on plasma coenzyme Q10 concentrations and semen quality in stallions. Journal of Equine Veterinary Science, 96, 103303. 10.1016/j.jevs.2020.103303
doi: 10.1016/j.jevs.2020.103303pubmed: 33349408google scholar: lookup
nnSaraswat, S.n, nKharche, S. D.n, & nJindal, S. K.n (2014). Impact of reactive oxygen species on spermatozoa: A balancing act between beneficial and detrimental effects. Iranian Journal of Applied Animal Science, 4(2), 247u2013255. http://ijas.ir/main/uploads/userfilesn
Sharma, A. , Minhas, S. , Dhillo, W. S. , & Jayasena, C. N. (2021). Male infertility due to testicular disorders. The Journal of Clinical Endocrinology & Metabolism, 106(2), 442u2013459. 10.1210/clinem/dgaa781
doi: 10.1210/clinem/dgaa781pmc: PMC7823320pubmed: 33295608google scholar: lookup
Usuga, A. , Rojano, B. , & Restrepo, G. (2017). Effect of seminal plasma components on the quality of fresh and cryopreserved stallion semen. Journal of Equine Veterinary Science, 58, 103u2013111. 10.1016/j.jevs.2017.09.005
doi: 10.1016/j.jevs.2017.09.005google scholar: lookup
Wilson, M. , & Flesner, A. T. (2017). A preliminary comparison of semen quality between competing and nonu2010competing equine stallions. Journal of Veterinary Science & Technology, 08(03), 443. 10.4127/2157-7579.1000443
doi: 10.4127/2157-7579.1000443google scholar: lookup