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Home / Equine Research Bank / BMC Vet Res / Effect of prolonged submaximal exercise on serum oxidative s...
BMC veterinary research2018; 14(1); 216; doi: 10.1186/s12917-018-1540-y

Effect of prolonged submaximal exercise on serum oxidative stress biomarkers (d-ROMs, MDA, BAP) and oxidative stress index in endurance horses.

Abstract: Oxidative stress (OS) associated with an intense exercise may have a negative influence on equine health. The aim of this study was to determine the effects of endurance races on oxidative and antioxidative status of horses by evaluating changes in reactive oxygen metabolites (d-ROMs), malondialdehyde (MDA), biological antioxidant potential (BAP) and oxidative stress index (OSI) values. The study was carried out on 53 race starts (28 individual horses) competing at different endurance races according to distance (40 and 80 km) and difficulty (easy and demanding). Blood samples were taken before and after the race. Results: Compared to levels of OS serum biomarkers before the race, an increase in values of d-ROMs (P <  0.01), MDA (P <  0.01), and BAP (P <  0.001), and a decrease in OSI (P <  0.001) have been noted after the race. Contrary to other measured biomarkers, BAP did not show significant individual effects of horses. Horses competing at shorter races have shown a significant change in d-ROMs (P = 0.002), BAP (P <  0.001) and OSI (P = 0.004), whereas those competing at longer races in MDA (P = 0.002), BAP (P <  0.001) and OSI (P <  0.001) post-race values. Endurance racing induced changes in values of d-ROMs, BAP and OSI during both easy and demanding races. Conclusions: Changes in all measured OS biomarkers indicate that prolonged aerobic exercise during endurance race could contribute to the imbalance between oxidants and antioxidants in horses, mainly characterised by a pronounced antioxidant response. Biological antioxidant potential was found to be the most reliable biomarker of OS in endurance horses in the present study.
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Publication Date: 2018-07-06 PubMed ID: 29980209PubMed Central: PMC6035461DOI: 10.1186/s12917-018-1540-yGoogle Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on understanding the impact of long-duration, moderate-intensity exercise on the oxidative stress levels in race horses, by examining certain biomarkers in their blood. The findings suggest that this kind of exercise can disrupt the balance between oxidants and antioxidants, leading to increased oxidative stress levels.

Research Objective and Procedure

  • This study aimed to determine how endurance races impacted horses’ oxidative stress (OS) levels and antioxidant status. This was achieved by tracking changes in the horses’ blood levels of reactive oxygen metabolites (d-ROMs), malondialdehyde (MDA), biological antioxidant potential (BAP) and oxidative stress index (OSI).
  • The experiment was carried out on 53 race starts involving 28 horses, with each horse running in multiple races varying in distance and difficulty. Blood samples were collected for analysis before and after each race.

Results

  • The results of the samples taken before and after the race showed a significant increase in the values of d-ROMs, MDA, and BAP after the race, and a decrease in OSI, suggesting an increase in oxidative stress in the horses as a result of endurance racing.
  • Notably, the results indicated that BAP did not show significant individual effects on horses, unlike the other OS biomarkers measured.
  • It was also observed that the changes in measured biomarkers varied depending on the length of the race. For example, horses that participated in shorter races showed significant changes in d-ROMs, BAP, and OSI, while those in longer races showed significant changes in MDA, BAP, and OSI values after the race.
  • Importantly, changes in d-ROMs, BAP, and OSI were seen during both easy and challenging races.

Conclusions and Implications

  • The results indicate that prolonged low-intensity exercise during endurance races could disrupt the balance between oxidants and antioxidants in horses, mainly characterized by a significant antioxidant response.
  • BAP was found to be the most reliable biomarker for assessing oxidative stress in endurance horses in this study. This suggests BAP could serve as an efficient tool for monitoring and managing oxidative stress and overall health in racing horses.

Cite This Article

APA
Brkljača Bottegaro N, Gotić J, Šuran J, Brozić D, Klobučar K, Bojanić K, Vrbanac Z. (2018). Effect of prolonged submaximal exercise on serum oxidative stress biomarkers (d-ROMs, MDA, BAP) and oxidative stress index in endurance horses. BMC Vet Res, 14(1), 216. https://doi.org/10.1186/s12917-018-1540-y

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 14
Issue: 1
Pages: 216
PII: 216

Researcher Affiliations

Brkljača Bottegaro, Nika
  • Clinic for Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia. nikabottegaro@gmail.com.
Gotić, Jelena
  • Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.
Šuran, Jelena
  • Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.
Brozić, Diana
  • Department of Animal Nutrition and Dietetics, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.
Klobučar, Karla
  • Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.
Bojanić, Krunoslav
  • School of Veterinary Science, Massey University, Palmerston North, New Zealand.
Vrbanac, Zoran
  • Department of Radiology, Ultrasound Diagnostic and Physical Therapy, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.

MeSH Terms

  • Animals
  • Antioxidants / analysis
  • Biomarkers / blood
  • Female
  • Horses / blood
  • Horses / metabolism
  • Horses / physiology
  • Male
  • Malondialdehyde / blood
  • Oxidative Stress / physiology
  • Physical Conditioning, Animal / physiology
  • Reactive Oxygen Species / blood

Conflict of Interest Statement

ETHICS APPROVAL AND CONSENT TO PARTICIPATE: A written consent statement was obtained from the owners of animals included in the study. The Ethical Board of the Faculty of Veterinary Medicine approved the study (File No. 640–01/16–17/75; Record No. 251–61-01/139–16-2). CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Citations

This article has been cited 10 times.
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