Effect of scaffold dilution on migration of mesenchymal stem cells from fibrin hydrogels.
Abstract: To evaluate the effect of fibrin concentrations on mesenchymal stem cell (MSC) migration out of autologous and commercial fibrin hydrogels. Methods: Blood and bone marrow from six 2- to 4-year-old horses. Methods: Autologous fibrinogen was precipitated from plasma and solubilized into a concentrated solution. Mesenchymal stem cells were resuspended in fibrinogen solutions containing 100%, 75%, 50%, and 25% of the fibrinogen precipitate solution. Fibrin hydrogels were created by mixing the fibrinogen solutions with MSCs and thrombin on tissue culture plates. After incubation for 24 hours in cell culture medium, the MSCs that had migrated onto the tissue culture surface and beyond the boundary of the hydrogels were counted. This procedure was repeated with a commercial fibrin sealant. Results: Hydrogel-to-surface MSC migration was detected for all fibrin hydrogels. Migration from the 25% autologous hydrogels was 7.3-, 5.2-, and 4.6-fold higher than migration from 100%, 75%, and 50% autologous hydrogels, respectively. The number of migrating cells from 100%, 75%, and 50% autologous hydrogels did not differ significantly. With commercial fibrin sealant, the highest magnitude of migration was from the 25% hydrogels, and it was 26-fold higher than migration from 100% hydrogels. The 75% and 50% hydrogels resulted in migration that was 9.5- and 4.2-fold higher than migration from the 100% hydrogels, respectively. Conclusions: MSC migration from fibrin hydrogels increased with dilution of the fibrinogen component for both autologous and commercial sources. These data supported the feasibility of using diluted fibrin hydrogels for rapid delivery of MSCs to the surface of damaged tissues.
Publication Date: 2012-01-28 PubMed ID: 22280396DOI: 10.2460/ajvr.73.2.313Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research explores how the concentration of fibrin, a protein involved in blood clotting, in both autologous (from self) and commercial fibrin hydrogels, affects the movement of mesenchymal stem cells (MSCs). The findings indicate that the migration of MSCs from fibrin hydrogels into damaged tissues increases with decreasing fibrin concentration.
Objective of the Study
- The purpose of this study was to assess how the concentrations of fibrin in autologous and commercial fibrin hydrogels affected the migration of mesenchymal stem cells (MSCs). Fibrin hydrogels could potentially be used for delivering MSCs to damaged tissues, and the concentration of fibrin in the hydrogel could impact this delivery process.
Methods Used in the Research
- This study involved the collection of blood and bone marrow from six horses aged between 2 and 4 years.
- Autologous fibrinogen, a protein that converts to fibrin, was obtained from the plasma and dissolved into a concentrated solution.
- The MSCs were then resuspended in this fibrinogen solution at varying concentrations (100%, 75%, 50%, and 25%) and mixed with thrombin to create the fibrin hydrogels.
- The fibrinogen solutions with MSCs were placed on tissue culture plates to incubate for 24 hours, after which the number of MSCs that had migrated onto, and beyond, the surface of the tissue culture plates was counted.
- This procedure was also repeated using a commercially available fibrin sealant.
Research Findings
- The research identified MSC migration from all concentration samples of fibrin hydrogels, with higher migration rates associated with more dilute fibrin concentrations.
- In autologous hydrogels, the MSC migration rate from 25% concentration samples was between 4.6 to 7.3 times higher than migration rates from 50% to 100% concentration samples.
- In commercial fibrin sealants, migration rates from 25% concentration samples were significantly higher, at 26 times the rate from 100% concentration samples.
- There was no significant difference in MSC migration rates from 100%, 75%, and 50% fibrinogen concentration autologous hydrogels.
Conclusions
- The study concluded that decreasing the concentration of fibrin in fibrin hydrogels increases the migration of MSCs from the hydrogels, regardless of whether the fibrin is autologous or commercial.
- The findings support the potential use of diluted fibrin hydrogels for the rapid delivery of MSCs to damaged tissue surfaces, potentially aiding in tissue healing and regeneration.
Cite This Article
APA
Hale BW, Goodrich LR, Frisbie DD, McIlwraith CW, Kisiday JD.
(2012).
Effect of scaffold dilution on migration of mesenchymal stem cells from fibrin hydrogels.
Am J Vet Res, 73(2), 313-318.
https://doi.org/10.2460/ajvr.73.2.313 Publication
Researcher Affiliations
- Orthopaedic Research Center, Department of Clinical Science, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, 80523, USA.
MeSH Terms
- Animals
- Cell Movement / physiology
- Fibrin / chemistry
- Horses
- Hydrogels / chemistry
- Mesenchymal Stem Cells / cytology
- Mesenchymal Stem Cells / physiology
- Tissue Culture Techniques
- Tissue Scaffolds
Citations
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- Alshehri S, Susapto HH, Hauser CAE. Scaffolds from Self-Assembling Tetrapeptides Support 3D Spreading, Osteogenic Differentiation, and Angiogenesis of Mesenchymal Stem Cells. Biomacromolecules 2021 May 10;22(5):2094-2106.
- Commins J, Irwin R, Matuska A, Goodale M, Delco M, Fortier L. Biological Mechanisms for Cartilage Repair Using a BioCartilage Scaffold: Cellular Adhesion/Migration and Bioactive Proteins. Cartilage 2021 Dec;13(1_suppl):984S-992S.
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