Effects of glucosamine and chondroitin sulfate on mediators of osteoarthritis in cultured equine chondrocytes stimulated by use of recombinant equine interleukin-1beta.
Abstract: To determine whether glucosamine and chondroitin sulfate (CS) at concentrations approximating those achieved in plasma by oral administration would influence gene expression of selected mediators of osteoarthritis in cytokine-stimulated equine articular chondrocytes. Methods: Samples of grossly normal articular cartilage obtained from the metacarpophalangeal joint of 13 horses. Methods: Equine chondrocytes in pellet culture were stimulated with a subsaturating dose of recombinant equine interleukin (reIL)-1beta. Effects of prior incubation with glucosamine (2.5 to 10.0 microg/mL) and CS (5.0 to 50.0 microg/mL) on gene expression of matrix metalloproteinase (MMP)-1, -2, -3, -9, and -13; aggrecanase 1 and 2; inducible nitric oxide synthase (iNOS); cyclooxygenase (COX)-2; nuclear factor kappaB; and c-Jun-N-terminal kinase (JNK) were assessed by use of a quantitative real-time polymerase chain reaction assay. Results: Glucosamine at a concentration of 10 microg/mL significantly reduced reIL-1beta-induced mRNA expression of MMP-13, aggrecanase 1, and JNK. Reductions in cytokine-induced expression were also observed for iNOS and COX-2. Chondroitin sulfate had no effect on gene expression at the concentrations tested. Conclusions: Concentrations of glucosamine similar to those achieved in plasma after oral administration in horses exerted pretranslational regulation of some mediators of osteoarthritis, an effect that may contribute to the cartilage-sparing properties of this aminomonosaccharide. Analysis of results of this study indicated that the influence of CS on pretranslational regulation of these selected genes is limited or lacking.
Publication Date: 2005-12-13 PubMed ID: 16334941DOI: 10.2460/ajvr.2005.66.1861Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research studied the impact of glucosamine on the gene expression of osteoarthritis mediators in horse articular chondrocytes (cartilage cells). It revealed that glucosamine, at equivalent levels to those in plasma after oral administration, could regulate specific osteoarthritis mediators, potentially enhancing the capability of glucosamine to preserve cartilage.
Research Methodology
- The study used samples of normal articular cartilage from the metacarpophalangeal joints of 13 horses.
- Equine chondrocytes, grown in pellet culture, were stimulated with a subsaturation dose of recombinant equine interleukin (reIL)-1beta. This process was done to replicate the conditions of osteoarthritis in vitro.
- Subsequently, effects of exposing the cells to glucosamine (in concentrations of 2.5 to 10.0 micrograms/ml) and chondroitin sulfate (in concentrations of 5.0 to 50.0 micrograms/ml) were studied. The concentrations used were comparable to those achieved in plasma through oral administration.
- The impact on gene expression of matrix metalloproteinase (MMP)-1, -2, -3, -9, and -13; aggrecanase 1 and 2; inducible nitric oxide synthase (iNOS); cyclooxygenase (COX)-2; nuclear factor kappaB; and c-Jun-N-terminal kinase (JNK) was evaluated using a quantitative real-time polymerase chain reaction assay.
Research Results
- Glucosamine concentration of 10 micrograms/ml significantly reduced reIL-1beta-induced mRNA expression of MMP-13, aggrecanase 1, and JNK.
- Reductions in cytokine-induced expression were also observed for iNOS and COX-2, implying that glucosamine might have a possibility of altering the gene expression of key mediators of osteoarthritis.
- Chondroitin sulfate, on the other hand, did not show any influence on gene expression at the concentrations tested.
Conclusions
- Concentrations of glucosamine equivalent to those in horse plasma after oral administration were found to regulate some mediators of osteoarthritis pretranslationally, suggesting the potential cartilage-saving properties of glucosamine.
- The study results indicated that chondroitin sulfate’s influence on pretranslational regulation of these specific genes was limited or non-existent.
Cite This Article
APA
Neil KM, Orth MW, Coussens PM, Chan PS, Caron JP.
(2005).
Effects of glucosamine and chondroitin sulfate on mediators of osteoarthritis in cultured equine chondrocytes stimulated by use of recombinant equine interleukin-1beta.
Am J Vet Res, 66(11), 1861-1869.
https://doi.org/10.2460/ajvr.2005.66.1861 Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824, USA.
MeSH Terms
- ADAM Proteins / biosynthesis
- ADAM Proteins / genetics
- ADAMTS4 Protein
- Animals
- Cartilage, Articular / cytology
- Cartilage, Articular / drug effects
- Cartilage, Articular / enzymology
- Cartilage, Articular / metabolism
- Chondrocytes / drug effects
- Chondrocytes / enzymology
- Chondrocytes / metabolism
- Chondroitin Sulfates / pharmacology
- Cyclooxygenase 2 / biosynthesis
- Cyclooxygenase 2 / genetics
- Gene Expression / drug effects
- Glucosamine / pharmacology
- Horse Diseases / drug therapy
- Horses
- Interleukin-1 / pharmacology
- MAP Kinase Kinase 4 / biosynthesis
- MAP Kinase Kinase 4 / genetics
- Matrix Metalloproteinases / biosynthesis
- Matrix Metalloproteinases / genetics
- NF-kappa B / biosynthesis
- NF-kappa B / genetics
- Nitric Oxide Synthase Type II / biosynthesis
- Nitric Oxide Synthase Type II / genetics
- Osteoarthritis / drug therapy
- Osteoarthritis / genetics
- Osteoarthritis / metabolism
- Osteoarthritis / veterinary
- Procollagen N-Endopeptidase / biosynthesis
- Procollagen N-Endopeptidase / genetics
- Recombinant Proteins / pharmacology
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
Citations
This article has been cited 8 times.- Barbeau-Grégoire M, Otis C, Cournoyer A, Moreau M, Lussier B, Troncy E. A 2022 Systematic Review and Meta-Analysis of Enriched Therapeutic Diets and Nutraceuticals in Canine and Feline Osteoarthritis. Int J Mol Sci 2022 Sep 8;23(18).
- Kantor ED, O'Connell K, Liang PS, Navarro SL, Giovannucci EL, Du M. Glucosamine Use and Risk of Colorectal Cancer: Results from UK Biobank. Cancer Epidemiol Biomarkers Prev 2022 Mar 1;31(3):647-653.
- Kantor ED, O'Connell K, Du M, Cao C, Zhang X, Lee DH, Cao Y, Giovannucci EL. Glucosamine and Chondroitin Use in Relation to C-Reactive Protein Concentration: Results by Supplement Form, Formulation, and Dose. J Altern Complement Med 2021 Feb;27(2):150-159.
- D'Adamo S, Cetrullo S, Panichi V, Mariani E, Flamigni F, Borzì RM. Nutraceutical Activity in Osteoarthritis Biology: A Focus on the Nutrigenomic Role. Cells 2020 May 16;9(5).
- Kantor ED, Newton CC, Giovannucci EL, McCullough ML, Campbell PT, Jacobs EJ. Glucosamine use and risk of colorectal cancer: results from the Cancer Prevention Study II Nutrition Cohort. Cancer Causes Control 2018 Mar;29(3):389-397.
- van de Water E, Oosterlinck M, Dumoulin M, Korthagen NM, van Weeren PR, van den Broek J, Everts H, Pille F, van Doorn DA. The preventive effects of two nutraceuticals on experimentally induced acute synovitis. Equine Vet J 2017 Jul;49(4):532-538.
- Kantor ED, Zhang X, Wu K, Signorello LB, Chan AT, Fuchs CS, Giovannucci EL. Use of glucosamine and chondroitin supplements in relation to risk of colorectal cancer: Results from the Nurses' Health Study and Health Professionals follow-up study. Int J Cancer 2016 Nov 1;139(9):1949-57.
- Bascoul-Colombo C, Garaiova I, Plummer SF, Harwood JL, Caterson B, Hughes CE. Glucosamine Hydrochloride but Not Chondroitin Sulfate Prevents Cartilage Degradation and Inflammation Induced by Interleukin-1α in Bovine Cartilage Explants. Cartilage 2016 Jan;7(1):70-81.
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